Topical ABT-263 treatment reduces aged skin senescence and improves subsequent wound healing

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 19, 2024

Abstract Senescent cells (SnC) accumulate in aging tissues, impairing their ability to undergo repair and regeneration following injury. Previous research has demonstrated that targeting tissue senescence with senolytics can enhance by selectively eliminating SnCs specific aged tissues. In this study, we focused on SnC skin mice assess the effects subsequent wound healing. We applied ABT-263 directly of 24-month-old over a 5-day period. Following topical ABT-263, decreased gene expression senescent markers p16 p21, accompanied reductions SA-β-gal p21-positive compared DMSO controls. However, also triggered temporary inflammatory response macrophage infiltration skin. Bulk RNA sequencing ABT-263-treated revealed prompt upregulation genes associated healing pathways, including hemostasis, inflammation, cell proliferation, angiogenesis, collagen synthesis, extracellular matrix organization. Aged pre-treated exhibited accelerated closure. conclusion, effectively reduced several skin, thereby priming for improved This enhancement may be attributed ABT-263-induced senolysis which turn stimulates involved remodeling pathways.

Language: Английский

---

SenMayo transcriptomic senescence panel highlights glial cells in the ageing mouse and human retina

npj Aging, Journal Year: 2024, Volume and Issue: 10(1)

Published: Nov. 30, 2024

There is a growing need to better characterise senescent cells in the CNS and retina. The recently published SenMayo gene panel was developed identify transcriptomic signatures of senescence across multiple organ systems, but retina not included. While other approaches have identified retina, these largely focused on experimental models young animals. We therefore conducted detailed single-cell RNA-seq analysis cell populations different aged mice compared with five comprehensive human mouse brain transcriptome datasets. Transcriptomic were most apparent retinal glial cells, IL4, 13 10 AP1 pathway being prominent markers involved. Similar levels transcriptional observed glia old mice, whereas showed significantly increased enrichment scores advancing age.

Language: Английский

---

Topical ABT-263 treatment reduces aged skin senescence and improves subsequent wound healing

Aging, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 3, 2024

Senescent cells accumulate in aging tissues, impairing their ability to undergo repair and regeneration following injury. Previous research has demonstrated that targeting tissue senescence with senolytics can enhance by selectively eliminating SnCs specific aged tissues. In this study, we focused on senescent skin mice assess the effects subsequent wound healing. We applied ABT-263 directly of 24-month-old over a 5-day period. Following topical ABT-263, decreased gene expression markers p16 p21, accompanied reductions SA-β-gal- p21-positive compared DMSO controls. However, also triggered temporary inflammatory response macrophage infiltration skin. Bulk RNA sequencing ABT-263-treated revealed prompt upregulation genes associated healing pathways, including hemostasis, inflammation, cell proliferation, angiogenesis, collagen synthesis, extracellular matrix organization. Aged pre-treated exhibited accelerated closure. conclusion, effectively reduced several skin, thereby priming for improved This enhancement may be attributed ABT-263-induced senolysis which turn stimulates involved remodeling pathways.

Language: Английский

---