Mitochondrial haplogroups and variants defines differential severity of COVID-19 in the Pakistani population DOI
Ali Raza Bukhari, Yasir Rehman, Javaria Ashraf

et al.

Published: March 18, 2025

Abstract Mitochondria play a crucial role in cellular functions, including immune responses and their dysfunction is observed SARS-CoV-2-infected cells. Here we investigated mitochondrial DNA (mtDNA) variations COVID-19 severity, focusing on potential impact of variants and/or haplogroups. PCR cases (n = 147) healthy controls (HC; n 40) were recruited, mt-DNA was amplified, sequenced analyzed for haplogroups genetic variations. categorized as Critical severe (CS; 40), Moderate (MOD; 43) Asymptomatic/mild (AMD; 64) disease. Haplogroup analysis showed major haplogroup M negatively associated with CS Whereas U positive association W an increased AMD COVID-19. Furthermore, 46 significantly the identified. More importantly mt.5186A > T ND2 gene high mutpred score Overall, are its severity Pakistani population.

Language: Английский

Elevated serum mtDNA in COVID-19 patients is linked to SARS-CoV-2 envelope protein targeting mitochondrial VDAC1, inducing apoptosis and mtDNA release DOI Creative Commons
Anna Shteinfer‐Kuzmine, Ankit Verma, Rut Bornshten

et al.

APOPTOSIS, Journal Year: 2024, Volume and Issue: 29(11-12), P. 2025 - 2046

Published: Oct. 7, 2024

Mitochondria dysfunction is implicated in cell death, inflammation, and autoimmunity. During viral infections, some viruses employ different strategies to disrupt mitochondria-dependent apoptosis, while others, including SARS-CoV-2, induce host apoptosis facilitate replication immune system modulation. Given mitochondrial DNAs (mtDNA) role as a pro-inflammatory damage-associated molecular pattern inflammatory diseases, we examined its levels the serum of COVID-19 patients found it be high relative healthy donors. Furthermore, comparison protein profiles between individuals SARS-CoV-2-infected revealed unique bands patients. Using mass spectroscopy, identified over 15 proteins, whose were 4- 780-fold higher. As mtDNA release from mitochondria mediated by oligomeric form mitochondrial-gatekeeper-the voltage-dependent anion-selective channel 1 (VDAC1)-we investigated whether SARS-CoV-2 alters VDAC1 expression. Among three selected small envelope (E), nucleocapsid (N), accessory 3b E-protein induced overexpression, oligomerization, release. Additionally, this led dysfunction, evidenced increased ROS production cytosolic Ca

Language: Английский

Citations

4
Mitochondrial haplogroups and variants defines differential severity of COVID-19 in the Pakistani population DOI
Ali Raza Bukhari, Yasir Rehman, Javaria Ashraf

et al.

Published: March 18, 2025

Abstract Mitochondria play a crucial role in cellular functions, including immune responses and their dysfunction is observed SARS-CoV-2-infected cells. Here we investigated mitochondrial DNA (mtDNA) variations COVID-19 severity, focusing on potential impact of variants and/or haplogroups. PCR cases (n = 147) healthy controls (HC; n 40) were recruited, mt-DNA was amplified, sequenced analyzed for haplogroups genetic variations. categorized as Critical severe (CS; 40), Moderate (MOD; 43) Asymptomatic/mild (AMD; 64) disease. Haplogroup analysis showed major haplogroup M negatively associated with CS Whereas U positive association W an increased AMD COVID-19. Furthermore, 46 significantly the identified. More importantly mt.5186A > T ND2 gene high mutpred score Overall, are its severity Pakistani population.

Language: Английский

Citations

0