Hs1Cas12a and Ev1Cas12a confer efficient genome editing in plants DOI Creative Commons
Gen Li, Yingxiao Zhang,

Micah Dailey

et al.

Frontiers in Genome Editing, Journal Year: 2023, Volume and Issue: 5

Published: Oct. 12, 2023

Cas12a, also known as Cpf1, is a highly versatile CRISPR-Cas enzyme that has been widely used in genome editing. Unlike its well-known counterpart, Cas9, Cas12a unique features make it efficient editing tool at AT-rich genomic regions. To enrich the CRISPR-Cas12a plant toolbox, we explored 17 novel orthologs for their capabilities plants. Out of them, Ev1Cas12a and Hs1Cas12a showed multiplexed rice tomato protoplasts. Notably, exhibited greater tolerance to lower temperatures. Moreover, generated up 87.5% biallelic T0 Both achieved effective poplar plants, with 100% plants edited, albeit high chimerism. Taken together, demonstrated by both monocot dicot highlights potential promising tools species beyond.

Language: Английский

Multiplexed in-situ mutagenesis driven by a dCas12a-based dual-function base editor DOI Creative Commons
Yaokang Wu, Yang Li, Yanfeng Liu

et al.

Nucleic Acids Research, Journal Year: 2024, Volume and Issue: 52(8), P. 4739 - 4755

Published: April 3, 2024

Abstract Mutagenesis driving genetic diversity is vital for understanding and engineering biological systems. However, the lack of effective methods to generate in-situ mutagenesis in multiple genomic loci combinatorially limits study complex functions. Here, we design construct MultiduBE, a dCas12a-based multiplexed dual-function base editor, an all-in-one plasmid performing combinatorial mutagenesis. Two synthetic effectors, duBE-1a duBE-2b, are created by amalgamating functionalities cytosine deaminase (from hAPOBEC3A or hAID*Δ ), adenine TadA9), crRNA array processing dCas12a). Furthermore, introducing separator Sp4 minimizes interference array, thereby facilitating both Escherichia coli Bacillus subtilis. Guided corresponding arrays, MultiduBE successfully employed cell physiology reprogramming metabolic regulation. A novel mutation conferring streptomycin resistance has been identified B. subtilis incorporated into mutant strains with antibiotic resistance. Moreover, surfactin riboflavin titers improved 42% 15-fold, respectively, compared control single gene mutation. Overall, provides convenient efficient way perform

Language: Английский

Citations

6
CRISPR/Cas System: Applications and Prospects for Maize Improvement DOI
Yilin Jiang, Kangtai Sun,

Xueli An

et al.

ACS Agricultural Science & Technology, Journal Year: 2022, Volume and Issue: 2(2), P. 174 - 183

Published: Jan. 11, 2022

Maize (Zea mays) is one of the most important cereal crops to secure food supplies in world. A growing world population requires new approaches facilitate and accelerate maize breeding. As a powerful tool, CRISPR/Cas system has revolutionized fields functional genomics crop Compared cross breeding transgenic breeding, genome-editing based on will bring opportunities rapidly cultivating novel varieties with desired characteristics. Here, we review applications CRISPR/Cas9 improvement. We also discuss potential technologies, such as base editors, prime multiplex genome editing toward future In addition, challenges for are discussed.

Language: Английский

Citations

22
Recent advancements in CRISPR/Cas technology for accelerated crop improvement DOI
Debajit Das, Dhanawantari L. Singha, Ricky Raj Paswan

et al.

Planta, Journal Year: 2022, Volume and Issue: 255(5)

Published: April 23, 2022

Language: Английский

Citations

21
Pangenomics and Crop Genome Adaptation in a Changing Climate DOI Creative Commons
Jakob Petereit, Philipp E. Bayer, William J. W. Thomas

et al.

Plants, Journal Year: 2022, Volume and Issue: 11(15), P. 1949 - 1949

Published: July 27, 2022

During crop domestication and breeding, wild plant species have been shaped into modern high-yield crops adapted to the main agro-ecological regions. However, climate change will impact productivity in these regions, agriculture needs adapt support future food production. On a global scale, relatives grow more diverse environments than species, so may host genes that could adaptation of new variable environments. Through identification individuals with increased resilience we gain greater understanding genomic basis for this transfer crops. Pangenome analysis can help identify underlying stress responses harbouring untapped diversity relatives. The information gained from pangenomes then be applied towards breeding existing or re-domesticating crops, combining environmental traits productivity.

Language: Английский

Citations

20
Hs1Cas12a and Ev1Cas12a confer efficient genome editing in plants DOI Creative Commons
Gen Li, Yingxiao Zhang,

Micah Dailey

et al.

Frontiers in Genome Editing, Journal Year: 2023, Volume and Issue: 5

Published: Oct. 12, 2023

Cas12a, also known as Cpf1, is a highly versatile CRISPR-Cas enzyme that has been widely used in genome editing. Unlike its well-known counterpart, Cas9, Cas12a unique features make it efficient editing tool at AT-rich genomic regions. To enrich the CRISPR-Cas12a plant toolbox, we explored 17 novel orthologs for their capabilities plants. Out of them, Ev1Cas12a and Hs1Cas12a showed multiplexed rice tomato protoplasts. Notably, exhibited greater tolerance to lower temperatures. Moreover, generated up 87.5% biallelic T0 Both achieved effective poplar plants, with 100% plants edited, albeit high chimerism. Taken together, demonstrated by both monocot dicot highlights potential promising tools species beyond.

Language: Английский

Citations

12