Employment of RNA Interference and CRISPR/Cas Machinery for Disease Resistance in Crops

Published: May 9, 2025

Language: Английский

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Knockdown of hexokinase in Diaphorina citri Kuwayama (Hemiptera: Liviidae) by RNAi inhibits chitin synthesis and leads to abnormal phenotypes

Pest Management Science, Journal Year: 2022, Volume and Issue: 78(10), P. 4303 - 4313

Published: June 22, 2022

Silencing specific genes in pests using RNA interference (RNAi) technology is a promising new pest-control strategy. The Asian citrus psyllid, Diaphorina citri Kuwayama, the most important pest because it transmits Candidatus Liberibacter asiaticus, which causes huanglongbing. Chitin essential for insect development, and enzymes this pathway are attractive targets control.The hexokinase gene DcHK was characterized from D. to impair proper growth chitin synthesis through RNAi. transcription of more highly developed third-instar nymphs, adults Malpighian tube. RNAi needed dose-dependent, with 600 ng μl-1 dsDcHK sufficient knockdown endogenous expression. messenger (mRNA) level lowest at 36 h after dosing, there were significant effects on relative levels mRNA (DcTre, DcG6PI, DcGNAT, DcGFAT, DcPGM, DcUAP DcCHS), leading mortality, reduced body weight abnormal or lethal phenotypes.RNAi can be triggered by orally delivered double-stranded citri. These results provide support HK as potential target psyllid control. © 2022 Society Chemical Industry.

Language: Английский

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Gene silencing for invasive paper wasp management: Synthesized dsRNA can modify gene expression but did not affect mortality

PLoS ONE, Journal Year: 2023, Volume and Issue: 18(1), P. e0279983 - e0279983

Published: Jan. 3, 2023

Invasive paper wasps such as Polistes dominula are a major pest and problem for biodiversity around the globe. Safe highly targeted methods control of these other social wasp populations needed. We attempted to identify potentially-lethal gene targets that could be used on adult in silencing or RNA interference (RNAi) approach. Double-stranded (dsRNA) was designed target genes which has proven lethal insects. dsRNA provided either orally foragers directly injected into wasps. also unprotected protected from degradation by gut nucleases two different forms (lipofectamine carbon quantum dots). The effects oral delivery 22 forager evaluated. expression five successfully reduced following ingestion injection. These included FACT complex subunit spt16 (DRE4) RNA-binding protein fusilli (FUSILLI), both have been previously shown potential However, we found no evidence significant increases mortality injection when compared with treatments our experiments. protect digestive altered but similarly did not influence mortality. Our results indicate while many same can silenced induce insects, RNAi approaches may useful control.

Language: Английский

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Efficient nanoparticle‐based CRISPR‐Cas13d induced mRNA disruption of an eye pigmentation gene in the white‐backed planthopper, Sogatella furcifera

Insect Science, Journal Year: 2023, Volume and Issue: 30(6), P. 1552 - 1564

Published: May 18, 2023

The discovery of the clustered regularly interspaced short palindromic repeat (CRISPR) system has driven gene manipulation technology to a new era with applications reported in organisms that span tree life. utility CRISPR-mediated editing was further expanded mRNA following identification RNA-targeting Cas13 family smaller endonuclease proteins. Application this insect research, however, been more limited. In study, smallest member, Cas13d, and guide RNAs (gRNAs) were complexed versatile nanomaterial (star polycation, SPc) generate proof-of-concept RNA-editing platform capable disrupting expression eye pigmentation tryptophan 2,3-dioxygenase (SfTO) white-backed planthoppers (WBPHs). resulting red-eye phenotype present 19.76% (with 22.99% (without treatment groups comparable generated conventional RNA interference knockdown (22.22%). Furthermore, Cas13/gRNA manifested quickly than interference. Consistent expected Cas13d mechanism, SfTO transcript levels significantly reduced. Taken together, results indicate SPc-CRISPR-Cas13d/gRNA complex negatively impacted target gene. These findings confirm novel disruption insects lay foundation for development these tools implementation green agricultural pest management tactics.

Language: Английский

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Topical delivery of dsRNA in two hemipteran species: Evaluation of RNAi specificity and non-target effects

Pesticide Biochemistry and Physiology, Journal Year: 2022, Volume and Issue: 189, P. 105295 - 105295

Published: Nov. 19, 2022

Language: Английский

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