Common bean wild relatives: untapped source of novel genetic resistance to stress factors

Genetic Resources and Crop Evolution, Journal Year: 2025, Volume and Issue: unknown

Published: March 23, 2025

Language: Английский

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Exploring the genomic diversity and breeding applications of the Solanum sessiliflorum transcriptome via phylogenetic analysis

Crop Science, Journal Year: 2025, Volume and Issue: 65(2)

Published: March 1, 2025

Abstract Compelling evidence supports the potential application of wild crop relatives in Solanum breeding. Efforts have been made to generate genomic data from plants assess insertion advantageous traits into species. South America hosts a broad range that not widely evaluated at molecular level. sessiliflorum is species shows tolerance Ralstonia solanacearum and nematode infections. The Illumina platform was used construct transcriptome S. . were analyzed using bioinformatics tools for both phylogenetic comparison bioprospecting disease‐related genes. Our leaf assembly enables comparisons stress‐tolerant gene bioprospecting. De novo generated 114,184 unigenes. A unigene dataset with other nucleotide resources revealed greater similarity tuberosum than melongena Additionally, within Leptostemonum group, along , possesses features related clade evolution. Bioprospection disease response targets identified 122 candidate unigenes retrieved dataset. abundant expression fragments hormonal defense genes appears constitute housekeeping mechanism avoid pathogen attacks on leaves. In total, 1091 classified as transcription factors (TFs), large number TFs associated biotic resilience. These results highlight exploring diversity which will be useful applications breeding programs.

Language: Английский

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A wheat CC-NBS-LRR protein Ym1 confers WYMV resistance by recognizing viral coat protein

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 16, 2025

Abstract Ym1 is the most widely utilized gene for wheat yellow mosaic virus (WYMV) disease control in worldwide breeding. Here, we successfully isolated responsible . It encodes a typical CC-NBS-LRR type R protein, which specifically expressed root and induced upon WYMV infection. -mediated resistance likely achieved by blocking viral transmission from cortex into steles, thereby preventing systemic movement to aerial tissues. CC domain essential triggering cell death. interacts with coat this interaction leads nucleocytoplasmic redistribution, process transitioning an auto-inhibited activated state. The activation subsequently elicits hypersensitive responses establishes resistance. introgressed sub-genome X n or c of polyploid Aegilops species. findings highlight exogenous-introgressed root-specifically that confers recognizing component.

Language: Английский

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Utilizing genetic variation in perennial sorghum to improve host plant resistance to aphids

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 19, 2025

With growing concerns over the sustainability of conventional farming systems, perennial crops offer an environmentally friendly and resilient alternative for long-term agricultural production. Perennial grain provide numerous benefits, such as low input investment, reduced tillage, soil conservation, better carbon sequestration, sustainable yields, enhanced biodiversity support. Sorghum (Sorghum bicolor) is fifth most-grown cereal crop grown food, fuel, food in world. The development sorghum offers a substitute traditional annual by providing environmental, economic, agronomic benefits. Sugarcane aphid (SCA; Melanaphis sacchari), phloem-feeder, considered major threat to Since its first report 2013, it caused $40.95 million losses South Texas alone 2015, accounting about 19% total value production region. In this study, we screened diverse genotypes using no-choice choice assays determine their innate antibiosis antixenosis resistance levels SCAs. Based on reproduction plant damage rating, bioassay classified 43 into four clusters: highly susceptible, moderately resistant, resistant. To further investigate mechanisms, selected two genotypes, X999 > R485 (SCA-resistant) PR376 ~ Tift241 (SCA-susceptible) that showed greatest variation SCA, subsequent experiments. Choice results indicated aphids chose settlement, whereas no significant preference was observed compared control genotype. Electrical penetration graph (EPG) demonstrated feeding SCA-resistant genotype spent significantly less time phloem phase than susceptible plants. identification will be valuable future breeding programs managing economically important pest.

Language: Английский

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Supporting crop plant resilience during climate change

Crop Science, Journal Year: 2023, Volume and Issue: 63(4), P. 1816 - 1828

Published: May 22, 2023

Abstract The changing climate poses significant threats to agriculture and the ability ensure sufficient global food production. With expanding population, there is an urgent demand increase crop productivity meet rising demand. Producing climate‐smart varieties together with developing new agronomic management strategies are that may help address this issue. Recent advances in genomics‐assistant breeding, use of high‐throughput DNA sequencing, high‐resolution phenotyping, advanced genome engineering can support development advanced, resilient crops. Here, we assess potential enhance resilience crops under climate. Through big data, breeding strategies, practices, could be produced enhanced increased nutrition, supporting future security.

Language: Английский

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CRISPR-Based Genome Editing Tools: An Accelerator in Crop Breeding for a Changing Future

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(10), P. 8623 - 8623

Published: May 11, 2023

Genome editing is an important strategy to maintain global food security and achieve sustainable agricultural development. Among all genome tools, CRISPR-Cas currently the most prevalent offers promise. In this review, we summarize development of systems, outline their classification distinctive features, delineate natural mechanisms in plant exemplify applications research. Both classical recently discovered systems are included, detailing class, type, structures functions each. We conclude by highlighting challenges that come with offer suggestions on how tackle them. believe gene toolbox will be greatly enriched, providing new avenues for a more efficient precise breeding climate-resilient crops.

Language: Английский

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Association study between some cultivated species and their wild relatives from Apiaceae, Asteraceae and Brassicaceae families based on molecular and DNA barcoding in Egypt

Genetic Resources and Crop Evolution, Journal Year: 2023, Volume and Issue: 71(3), P. 1125 - 1143

Published: Aug. 13, 2023

Abstract Concerns about food security worldwide have been raised due to the adverse impacts of climate change on agricultural productivity. Utilizing wild relatives cultivated plants in breeding schemes is a potential solution address abovementioned challenges. Wild refer indigenous plant species that share genetic ancestry with recently plants. The capacity withstand abiotic stresses and enhance crop yield stability cold arid environments renders them valuable prospective resource for programs. Molecular markers are employed assess variability their under investigation. This study set five primers derived from ISSR (Inter Simple Sequence Repeats) SCoT (Start Codon Targeted) comparative research between three families: Apiaceae ( Apium gravolens L., A. leptophyllum (Pers.) F.Muell., Daucus carota L . & D. litoralis Sm.), Asteraceae Lactuca sativa L. serriola L.) Brassicaceae B. oleracea Brassica tourneforttii Gouan., Raphanus sativus R. raphanistrum L.). technique yields approximately 206 bands, whereas generate 207 bands. yielded 169 polymorphic exhibiting polymorphism rate 82.04%, produced bands 81.64%. has found exhibit highest resolving power (RP) 48.25, as determined by ISSR. primary objective DNA barcoding create communal database sequences can be utilized identify organisms clarify taxonomic classification. findings indicate internal transcribed spacer (ITS) regions high efficacy tool. Overall, results obtained two molecular analyses suggest significant degree diversity relatedness among taxa examined this study.

Language: Английский

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Realizing the potential of plant genetic resources: the use of phenomics for genebanks

Plants People Planet, Journal Year: 2024, Volume and Issue: 7(1), P. 23 - 32

Published: Sept. 12, 2024

Societal Impact Statement Genebanks contribute to global food security, directly influencing societal well‐being, by providing access seed or genetic material that is more resilient pests, diseases, and environmental stresses. The ability develop crops adapted changing conditions with high nutritional value means societies can better cope the adverse effects of climate change, safeguarding production rural livelihoods. Enhancing sustainable crop traits, such as improved water‐use efficiency nutrient utilization, supports transition toward agricultural practices. New technologically advanced phenotyping tools are required optimally realize potential these resources, identify plant resources will best enable us address current challenges. Summary Plant genebanks have a crucial role specialized repositories, preserving diverse essential researchers, breeders, farmers for developing crops. With over 7 million accessions, significantly change mitigation, agriculture. This opinion paper, inspired an international workshop in 2022 Wageningen (Netherlands), focuses on transformative impact phenomics technology within genebanks, offering view its applications implications utilizing resources. Discussions from organized International Phenotyping Network integrated, emphasizing collaboration without requiring major policy developments. prioritized traits like disease resistance drought tolerance, highlighting roots critical organ phenotyping. Participants expressed keen interest collaboration, need partnerships between facilities. workshop's outcomes underscore promoting innovation, These results guide future pilot studies, marking significant step integrating into genebank practices ensuring continued prosperity heritage.

Language: Английский

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Exploring Pan-Genomes of Crops: Genomic Resources and Tools for Unraveling Gene Evolution, Function, and Adaptation

Published: Aug. 23, 2023

The availability of multiple sequenced genomes from a single species made it possible to explore intra- and inter-specific genomic comparisons at higher resolution build clade-specific pangenomes several crops. pan-genomes crops constructed various cultivars/accessions, landraces, wild ancestral represent compendium genes structural variations allow researchers search for the novel alleles that were inadvertently lost in domesticated during historical process crop domestication or extensive plant breeding. Fortunately, many valuable associated with desirable traits like disease resistance, abiotic stress tolerance, architecture, nutrition qualities exist species, relatives. novels ancestors landraces can be introduced back high-yielding varieties modern by implementing classical breeding, selection, transgenic/gene editing approaches. Thus, pan-genomic represents great leap research offers new avenues targeted breeding mitigating impact global climate change. Here we summarize tools used pangenome assembly annotations, web-portals hosting pangenomes. Furthermore, highlight few discoveries using approach its future potential.

Language: Английский

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‘Omics’ Approaches for Crop Improvement

Agronomy, Journal Year: 2023, Volume and Issue: 13(5), P. 1401 - 1401

Published: May 19, 2023

The growing human population and climate change are imposing unprecedented challenges on the global food supply [...]

Language: Английский

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