Genetic manipulation for stress-tolerant plants: current status and challenges

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 341 - 363

Published: Jan. 1, 2025

Language: Английский

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Regulatory genes for the improvement of salt and drought tolerance

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 365 - 387

Published: Jan. 1, 2025

Language: Английский

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Development of transgenic wheat plants withstand salt stress via the MDAR1 gene

GM crops & food, Journal Year: 2025, Volume and Issue: 16(1), P. 173 - 187

Published: Feb. 10, 2025

In light of the fact that climate change has emerged as one difficulties confronting global food system, researchers are obligated to work toward developing fundamental crops, particularly wheat, combat environmental stress, including drought and salt. present study, genetic engineering was used transfer Arabidopsis MDAR1 gene, which controls buildup ascorbic acid (AsA) make bread wheat less likely be sensitive salt stress. The biolistic bombardment cDNA from thaliana plant encodes into Bobwhite 56 cultivar plants. A molecular investigation performed on six different transgenic lines confirm integration transgene, copy number, expression transgene. There were three copies there no association found between number transgene All data generated or analyzed during this study included in published article [and its supplementary information files].the presence expression. Compared plants not transgenic, amount accumulated twice high. ROS concentrations significantly lower compared non-transgenic under both control stress conditions, effectively reducing oxidative By cultivating T2 a greenhouse, we able determine whether they tolerate potentially damaging effects (200 mm). concluded consistently expressed gene become tolerant with improvement growth characteristics.

Language: Английский

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Genome-wide identification of SWEET family genes and functional analysis of NtSWEET12i under drought and saline-alkali stresses in tobacco

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 14, 2025

SWEET (Sugar Will Eventually be Exported Transporter) proteins play vital roles in the transport of sugars, contributing to regulation plant development, hormone signaling, and responses abiotic stress. In this study, we identified 57 NtSWEETs tobacco (Nicotiana tabacum L.), then their physicochemical properties, chromosomal localization, synteny, phylogenetic relationships, genomic structure, promoter cis-elements, protein interaction network, tissue specificity, expression pattern were systematically analyzed. addition, NtSWEET12i improves drought saline-alkali tolerance by enhancing soluble sugars transport, ABA proline biosynthesis, ROS scavenging. These findings illuminate role NtSWEETs, particularly NtSWEET12i, regulating stresses. This study offers new insights enhance our understanding identify potential genes involved plants. Not applicable.

Language: Английский

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Nanoparticle-driven stress alleviation: exploring the roles of metal and metal oxide nanoparticles in plant abiotic stress management

Discover Plants., Journal Year: 2025, Volume and Issue: 2(1)

Published: March 19, 2025

Abstract Nanoparticles have demonstrated significant potential in reducing the negative impacts of abiotic stress on plants and improving agricultural productivity. Temperature, salt, drought, heavy metals can hinder plant growth crop yields. Nanoparticles, mainly metal oxide nanoparticles been found to enhance resilience mitigate by affecting physiological molecular processes. Studies shown that improve growth, biomass, chlorophyll content, antioxidant production. They also nutrient absorption, regulate osmotic balance, activate defense mechanisms. like zinc (ZnO), silicon (Si), iron (FeO) effective mitigating salt stress. In contrast, manganese (Mg) titanium dioxide (TiO 2 ) promise alleviating drought Additionally, reduce toxicity heat tolerance. However, using agriculture requires careful consideration their risks environmental impact. provide a promising opportunity for enhancing sustainable practices face climate change food security challenges. By leveraging nanotechnology, we farm productivity secure resources future generations. Graphical abstract

Language: Английский

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Mechanistic Understanding of GABA and Trehalose in Modulating Plant Response to Drought Stress

Plant Stress, Journal Year: 2025, Volume and Issue: unknown, P. 100838 - 100838

Published: April 1, 2025

Language: Английский

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Omics and CRISPR-Cas9 molecular perception: A progressive review approach for powdery mildew disease management

Physiological and Molecular Plant Pathology, Journal Year: 2024, Volume and Issue: 130, P. 102217 - 102217

Published: Jan. 2, 2024

Language: Английский

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Single-cell proteomics of Arabidopsis leaf mesophyll identifies drought stress-related proteins

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 3, 2024

Abstract The application of single-cell omics tools to biological systems can provide unique insights into diverse cellular populations and their heterogeneous responses internal external perturbations. Thus far, most studies in plant have been limited RNA-sequencing approaches, which only indirect readouts functions. Here, we present a proteomics workflow for cells that integrates tape-sandwich protoplasting, piezoelectric cell sorting, nanoPOTS sample preparation, FAIMS-based MS data acquisition method label-free analysis Arabidopsis leaf mesophyll cells. From single protoplast, over 3,000 proteins were quantified with high precision. is demonstrated identify stress associated changes protein abundance by analyzing >80 protoplasts from well-watered water-deficit stressed plants. Additionally, describe new approach constructing covarying networks at the level demonstrate how covariation reveal previously unrecognized functions while also capturing stress-induced protein-protein dynamics. Highlights This study describes first scProteomics abiotic proteome regulation ∼2800 on average precision using label free leaves revealed known novel involved drought response Single-protoplast water deficit-induced independent

Language: Английский

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Helicase: A genetic tool for providing stress tolerance in plants

Plant Stress, Journal Year: 2023, Volume and Issue: 9, P. 100171 - 100171

Published: June 3, 2023

Alteration of stress (abiotic and biotic) constitutes one the major problems for normal plants health. In present scenario, new molecular tools are broadly utilized to improve tolerance high-yielding crop plants. Helicases such proteins that as largest conserved gene families in eukaryotes, prokaryotes, All members this family involved DNA RNA metabolism inside cell which maintains cellular functions. Environmental reduces plant's growth yield causes severe global food losses. To overcome stress-inducing losses, a stress-tolerant trait is required security. process nucleic acid act potential molecules abiotic Both helicase highly influences defense mechanism protects from pathogen infection other factors Helicase enzyme classified into six superfamilies SF1 SF6. protein acts motor. uses energy nucleoside 5′-triphosphate (NTP) unwinding energetic stable duplex single strands. helicases have some common properties. Those binding, hydrolysis, NTP ds 3′ 5′ or direction. mainly plants' nuclei, mitochondria, chloroplasts. But recently researchers found plant an important role develop healthy high yielding under stressful environments. Unlike helicase, large group biochemical activities, including unwinding, displacement RNA, strand annealing. DEAD-box metabolisms like review, we summarized discussed structure, function, different crops.

Language: Английский

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CRISPR/Cas9-Targeted Disruption of Two Highly Homologous Arabidopsis thaliana DSS1 Genes with Roles in Development and the Oxidative Stress Response

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(3), P. 2442 - 2442

Published: Jan. 26, 2023

Global climate change has a detrimental effect on plant growth and health, causing serious losses in agriculture. Investigation of the molecular mechanisms responses to various environmental pressures generation plants tolerant abiotic stress are imperative modern science. In this paper, we focus application well-established technology CRISPR/Cas9 genome editing better understand functioning intrinsically disordered protein DSS1 response oxidative stress. The Arabidopsis contains two highly homologous genes, AtDSS1(I) AtDSS1(V). This study was designed identify functional differences between AtDSS1s, focusing their potential roles We generated single dss1(I) dss1(V) mutant lines both genes using technology. homozygous with large indels (dss1(I)del25 dss1(V)ins18) were phenotypically characterized during development sensitivity analyzed. characterization revealed root stem lengths, rosette area size. Plants disrupted AtDSS1(V) gene exhibited lower survival rates increased levels oxidized proteins comparison WT exposed induced by hydrogen peroxide. work, dss1 double not obtained due embryonic lethality. These results suggest that DSS1(V) could be an important component response.

Language: Английский

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