Plant-microbe interactions in the rhizosphere for smarter and more sustainable crop fertilization: the case of PGPR-based biofertilizers

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 8, 2024

Biofertilizers based on plant growth promoting rhizobacteria (PGPR) are nowadays gaining increasingly attention as a modern tool for more sustainable agriculture due to their ability in ameliorating root nutrient acquisition. For many years, most research was focused the screening and characterization of PGPR functioning nitrogen (N) or phosphorus (P) biofertilizers. However, with increasing demand food using far fewer chemical inputs, new investigations have been carried out explore potential use such bacteria also potassium (K), sulfur (S), zinc (Zn), iron (Fe) In this review, we update biofertilizers smarter crop production deliberate prospects microbiome engineering-based methods tools shed light improvement mineral nutrition. The current era omics revolution has enabled design synthetic microbial communities (named

Language: Английский

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Inoculation of phosphate-solubilizing bacteria improves soil phosphorus mobilization and maize productivity

Nutrient Cycling in Agroecosystems, Journal Year: 2023, Volume and Issue: 126(1), P. 21 - 34

Published: March 31, 2023

Abstract Phosphate-solubilizing bacteria represent a bioalternative in making soil-immobilized phosphorus (P) available to plants, and consequently improve agriculture sustainability reduce nutrient pollution. In this study, we examined whether Rhizobium sp. B02 inoculation can affect the soil P fractions. Moreover, investigated how influences growth, physiological traits, productivity of maize crop. Field tests were carried out evaluate combined application strain reduced doses fertilizer. Soil fractionation was performed after crop harvesting, assessing dynamics. To study plant response, samplings three phenological stages—the vegetative stage 7 fully emerged leaves with leaf collars (V7), tassel (VT), reproductive maturity (R6). Using 50% fertilizer recommended, increased labile inorganic fraction by 14% compared control treatment at same dose, indicating that it favored Pi mobility. Under these conditions V7 VT stages, significantly improved shoot length (28 3%) dry weight (9.8 12%). grain yield 696 kg ha −1 using recommended rate fertilizer, phenocopying complete fertilization without inoculation. Therefore, replaced availability.

Language: Английский

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Phosphate-solubilizing bacteria increase maize phosphorus uptake from magnesium-enriched poultry manure biochar

Biology and Fertility of Soils, Journal Year: 2024, Volume and Issue: 60(3), P. 421 - 436

Published: March 12, 2024

Language: Английский

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Phenological stages of wheat modulate effects of phosphorus fertilization in plant-soil microbial interactions

Plant and Soil, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 6, 2024

Abstract Aims Future phosphorus (P) fertilizer availability faces challenges due to limited phosphate rock mines and strict quality regulations regarding Cd contents in rock. In this study, conventional fertilization was partially substituted with meat bone meal (MBM), sludge (S), the organo-mineral combination of S plus MBM (SMBM), a wheat agroecosystem. Methods We investigated impact treatments crop phenological stages on P availability, yield, soil microbial responses. Analysis included enzyme activities, biomass, composition bacterial fungal communities using metabarcoding. Additionally, we estimated functional genes related cycle through qPCR. Crop yield nutrient content plants were also determined. Results Replacing traditional SMBM maintained at levels equivalent fertilization. produced 70% 40% (respectively) more bioavailable compared treatment (Trad). Significant differences between biomass observed flag leaf stage. increased 20% total Trad. phenology had stronger than treatments. The use enhanced activity. Yield both plots exhibited no statistically significant Conclusion Organo-mineral emerges as sustainable strategy for maintaining production while improving functionality. Our findings emphasize primary influence shaping influencing

Language: Английский

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Phosphorus Availability and its Sustainable Use

Journal of soil science and plant nutrition, Journal Year: 2022, Volume and Issue: 22(4), P. 5036 - 5048

Published: Sept. 1, 2022

Language: Английский

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(Poly)phosphate biotechnology: Envisaged contributions to a sustainable P future

Microbial Biotechnology, Journal Year: 2023, Volume and Issue: 16(8), P. 1616 - 1622

Published: March 28, 2023

The overall challenge of establishing a sustainable anthropogenic phosphate cycle did not change in the last decade, rather need to act becomes ever more urgent. Here, I briefly highlight some developments (poly)phosphate research within 10 years and dare crystal ball look for topics that might help us towards P society. Ten after review series on biotechnology (Shilton & Blank, 2012), it is time upcoming technologies have potential slow down our linear Pi use—from mining rock, human Pi-applications finally Pi-sedimentation ocean. You may also want refer excellent by Elser (2012), which set stage series, covering many aspects value chain. Certainly, decade (Scholz et al., 2013), Indeed, yearly amount shipping keeps increasing (from 65 million metric tonnes 2012 72 2020), with regional distribution strongly biased Morocco. Predictions regarding volume peak usage rock centuries are neither good guides political decision-making new policies recycling nor industrial invest into alternative technologies. With number livestock provide growing world population, challenges environment terms sufficient but excessive quantities will get smaller soon. urbanization observed all parts might, however, be an opportunity increase recycling. countries instated regulations wastewater treatment plants only reduce residual treated water below certain threshold concentration recover accumulated from microbial biomass or ash incineration. For example, Germany released novel regulation Pi-recovery plants. From 2029 onwards, 50% sludge 80% recovered form enables reuse as, fertilizer. announcement catalysed between (novel) partners. A favoured strategy mono-combustion purification ash. Two different currently built announced capacities 20.000 t/year phosphoric acid Remondis 60.000 Pi-fertilizer Seraplast. An EU-wide recovery wastewater, has been yet. technological truly mind-blowing, authors even question entire system (first enormous dilution, followed somewhat cumbersome concentration), although monoburned seems feasible, as examples Seraplast indicate. straightforward saving would reduction protein production using traditional farming animals. Although we see stronger movement vegetarian vegan lifestyles industrialized countries, growth meat still considerable, mainly driven economic success societies. money spend, humans tend eat (Vranken 2014), development at easy reverse. fear relying behavioural alone insufficient, hence, require sources animal identical proteins. Such alternatives exist, patties stem cells entering market slowly surely (Balasubramanian 2021). proteins microbes attracting much attention. Some products already market, especially dairy industry. Microbial casein allows manufacturing ice creams other (e.g. Perfect Day Inc.). reducing Pi-use agricultural sector huge, requires potpourri approaches including soil erosion avoiding field pilling, minimizing food wastage, meat-heavy diets developed valorizing side streams utilization. asked less than fully revised (Elser, 2012). mined should used long possible, often referred cascade use, manure fertilizer very example. Alternative Pi-sources possibilities use were reviewed recently (Vučić Müller, Understanding roles organisms strategies exist thrive under scarcity (Tapia-Torres 2016) now focus ongoing research. While polyP ancient molecule characterized proposed functions, few seen tremendous interest community facets biology. Prominent polyP-modified (Azevedo 2015) well direct role blood coagulation pathway mammals (Rangaswamy common inorganic class polyPs united researchers first conference planned 2020 was delayed due Corona pandemic virtual event 2021 (FEBS—The New Biology Polyphosphate Health Disease). covers such activities bone formation (ossification), enhanced biological phosphorus removal (EBPR) microbes. analytical prominent similar projects. Besides others, parameters amount/concentration average molecular weight (Christ, Smith, 2020). When working natural polymers, reliable analytics cumbersome. Quantification determination my own work hyaluronic acid, gamma-polyglutamate, biopolymers, experienced quantification, determination, can difficult (Figure 1). polyP, summarized comprehensive like mention two enzyme assays allow (1) quantification (i.e. how polyP; Christ 2018b) (2) chain length (Christ 2019). suitable μM mM concentrations. As both regular microtitre plates, sample throughput increased drastically, while no sophisticated equipment NMR HPLC systems required. best measurements results, extraction preparation 2018a). total measurement (Assay 1; functions via hydrolysis Saccharomyces cerevisiae exopolyphosphatase 1 (scPpx1p, EC 3.6.1.11) S. pyrophosphatase (scIpp1p, 3.6.1.1) Pi, assayed 2 min well-known colorimetric detection reagent. Without addition enzymes, concentrations quantified well. Subsequently, assay established, comprising (sum reported mole monomers per litre) (polyP polymer concentration, chains litre). calculated dividing concentration. one-pot based scPpx1p, leaving one polyP2 PolyP2 transformed enzymatic NADPH involves enzymes ATP sulfurylase (EC 2.7.7.4), hexokinase 2.7.1.1) glucose 6-phosphate dehydrogenase 1.1.1.49). fluorometrically. These substantially simplify analytics, thereby catalysing studies biology this intriguing polymer. polyphosphorylation particular importance phosphorylation post-translational modification known decades. It array signal transduction affinity protein–protein interactions. observation In 2015, Azevedo al. yeast type 2015). Since then, developed, indications play mammalian cells, despite generally low when compared Saiardi, 2016). covalent binding modifies lysine residues, located so-called PASK cluster, stretch amino acids rich polyacidic serine lysine. could clearly determined several possible targets published, these modifications, standard cell conditions concentrations, elusive. Interruption protein–DNA interactions likely. recent opinion paper, state art summarized, challenges, complicated highlighted (Baijal Downey, sure, next exciting understanding (Desfougères trait. interact multiple ways, additionally lysine, reports suggest drug, against SARS-CoV-2 infection, blocking virus–cell (Ferrucci All manuscripts start introduction information exists three forms: linear, cyclic branched. most applications PolyP about up 6 bought hexametaphosphate, content low. 1950, believed branched does aqueous hinted quickly hydrolyse (Van Wazer Holst, 1950). Dürr-Mayer showed otherwise (Dürr-Mayer Using classic chemical synthesis routes, they obtain determine rate material. However, if present any unknown. contrast, Mandala vivo non-destructive (Mandala suggesting previous Willbold, 2020) exclusively artefacts purification, indeed living cells. residues probably limited maximum 8. Binding proteins, ribonuclease NAD kinase, elusive investigated detail considering varying properties structurally polyPs. Moreover, origin material interest. Just recently, Glonek discussed had life (Glonek, degradation highly polyP. awareness species contributing molecules expected. selective task fulfil. incorporation biomass, excess converted energy storage. underlying mechanisms described bacteria, exploiting single components engineering (Hancock Hsu, 1996; Van Dien 1997). microalgae, response starvation allowed accumulation (Plouviez More ago, group Ohtake publications recovery, purification. suggested process named 'Heatphos,' bacterial achieved simple heating step 70°C. corresponding (Hirota 2010) valuable resource getting started, presentation pilot plant processing purified Dott presented combination bioleaching hyperaccumulation microbes, concluded cost substrate too high commercialization (Dott Michael, decades ago (Liss Langen, 1962). Recently, phenomenon optimized, resulting dry 28% (as KPO3) Briefly, yeasts grown batch mode, phase 2). step, population doubles, diluting significantly. After h, harvested transferred medium carbon source, nitrogen omitted. Under conditions, sustained, source however finances dear synthesis. 2–3 accumulate, depending strain. Yeast containing polyP-rich extract 2019) bio-polyP preparations, release intracellular polyp without degrading Notably, dedicated protocol 2018a), higher obtained protocols aimed large-scale summary, ideas upcycle PolyPs, important industries. small P-fertilizer least cover high-value niches decade. manifold, yield se. Also, cascading defined. Ruff phytases inositol (Herrmann sketching residues. sausage phytate 2023). year 2022, industry pressure, synthesized burning temperatures gas burner. running utilization large scale agro highest achieve short frame, touched paragraph. discussion fertilizing versus heart precision agriculture, evident there room improvement open field. soils exploited agriculture contain enough sustaining centuries. accessible hardly diffuses (estimated diffusion mm), rapidly trapped counterions, Fe2+, forming insoluble salts (Tian Hence, farmer year, composition tells otherwise. reason, mobilization time, secrete organic superior transporters Closer application phosphate-solubilizing bacteria (PSB) nourish initially P-salts. researched intensively, beneficial potentially cost-effective implement, benefit questioned. meta-study concludes PSB hampered influencing Pi-mobilization (De Zutter 2022). Especially pH crucial, overarching parameters. plenty 'food' solubilize obviously depends heavily targeted soil. general, monoseptic cultures, species, do perform worse mixtures identified consortia, competition resources limited. well-studied Bacilli Pseudomonads outperformed Burkholderia sp. described, same mobilizing ranked according stability constants respective Fe—or Al—organic complexes (Ryan 2001): citrate > oxalate malonate/malate tartrate lactate gluconate acetate formate. produced Aspergillus niger its ability rock. Up 75% mobilized detected just h (Mendes Paenibacilli promoting growth, their fixation. Paenibacillus sonchi strain solubilization (Brito combining central traits bacterium. study, hydroxyapatite proxy phosphate, indeed, bacterium producing gluconate. interesting P- N-fertilizer future. almost resources, demand linked disposable income is. know humanity decades, striving improve billions people, daunting task. encouraging biotechnology, improved upcycling polyphosphate sewage sludge. changing legislation asking efficiently. where farmers cannot afford fertilizers, certainly over dampened progress P-cascade world. prevent repetition mistakes previously made use. am sceptical find ways implement practices minimize environmental impacts. outlook, glance allowed. What happen CO2 green hydrogen agroindustry? Cai show synthetic chemo/bio-catalysis approach converts + H2 methanol further starch (Cai compare multistep catalysis maize—with eightfold rate—clearly demonstrating competitiveness parameter. Why sharing findings literature here? hydrolysis, feature seeing expecting extensively applied besides criteria, stability, ease advantages storage biotechnological ATP, omnipresent whole-cell catalysis. We fundamental demonstrated advanced diverse remain discovered. example bright evolutionary history, surprising, evolution explore physical features fascinating molecule. policy changes hopefully extended ever-increasing create opportunities biotechnology. Surely, knowledge reuse. great removing falling short. Increasing production. Challenges accumulating heavy metals considerable degradation. Imagine above-mentioned reaction starch, exceeds 115 growing. exceed manifold hence asks clear advantage, vitro runs contained reactors, enabling full P-recovery contrast today's corn, potato, rice wheat). optimistic efforts impacts increase, doubt lead Lars M. Blank: Conceptualization (lead); funding acquisition (equal); investigation project administration writing – original draft editing (equal). author grateful Jana Fees, Alexander Deitert Philipp Demling (all RWTH Aachen University) comments proofreading manuscript. Open Access enabled organized Projekt DEAL. This supported German Federal Ministry Education Research (BMBF) through MeY4bioPP—Metabolic baker's bio-polyphosphate (grant 031B0827A). declares commercial depicted submitted patent applications.

Language: Английский

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Phosphate Solubilizing Microorganisms Increase Soil Phosphorus Availability: A Review

Geomicrobiology Journal, Journal Year: 2023, Volume and Issue: 41(1), P. 1 - 16

Published: Oct. 24, 2023

Phosphorus (P) availability in soil is often limited, posing a challenge for sustainable agricultural production. Phosphate solubilizing microorganisms (PSMs) have emerged as promising biological approach to enhance P while minimize negative environmental impacts. Exploring the impact of PSMs on increasing available crucial optimizing utilization insoluble and promoting practices. In this article, we provide comprehensive review dynamics P, including its input, loss transformation processes. Additionally, draw conclusion about how increase soil, inorganic (Pi) organic (Po) by secreting substances such protons, acids, exopolysaccharides, hydrogen sulfide, siderophores phosphatases. Notably, factors influencing activity are thoroughly discussed. Moreover, practical applications summarized. Future research should focus exploring novel PSM isolates, conducting long-term field experiments assess effects potential risk under diverse conditions, investigating synergistic approaches with other activators or beneficial microorganisms. This will advance our understanding their application, enabling widespread adoption systems.

Language: Английский

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Occurrence of diverse plant growth promoting bacteria in soybean [Glycine max (L.) Merrill] root nodules and their prospective role in enhancing crop yield

Biocatalysis and Agricultural Biotechnology, Journal Year: 2024, Volume and Issue: 57, P. 103072 - 103072

Published: Feb. 17, 2024

Language: Английский

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Role of Beneficial Microorganisms in Vegetable Crop Production and Stress Tolerance

Published: Jan. 1, 2025

Language: Английский

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Tomato Production under Synergistic Application of Phosphate Solubilizing Bacteria and Phosphate Amendments

Advances in Agriculture, Journal Year: 2023, Volume and Issue: 2023, P. 1 - 14

Published: March 1, 2023

Phosphate solubilizing bacteria have multi-dimensional benefits in broad host range interaction, accessing nutrients, phytohormone induction, stress alleviation, biocontrol activity, and eco-friend approach. This study aimed to evaluate the efficacy of PSB isolates coinoculated with compost, bone meal, DAP fertilizer on tomato growth response. Tomato seeds were treated 10 selected separately grown for 20 days seedbed, then transplanted field that was external P-sources enriched by inoculum. showed positive interaction achieved significant plant assays including height, leaves, branches, flowers, fruit development. Isolate K-10-41 significantly promoted floral development, yield, Mk-20-7 enhanced height weight whereas K-10-27 induced numbers. Compost application tomato-PSB vegetative meal least promotor most assays. Bone however, one top development inducers (harvested 20.94 fruits/plant weighing 881.97 gm). Mixing 50% recommended compost yield (21 harvested weighed 872.46 Based overwhelming performance, together mixture found effective. Therefore, results this imply competent nutrient supplements improved symbiotic effectiveness, sustainable production, environmental health. Consequently, these promising would be production higher sustainability after verifying their at different agroecology taxonomic identification. Screening potential strains evaluating competence under conditions future perspectives develop efficient inoculants. Moreover, synergetic organic (compost, farmyard, or other biowastes), bioinoculants, proper agrochemicals maximize health is feasible economic, social, ecological sense balance.

Language: Английский

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