A linkage map of Aegilops biuncialis reveals significant genomic rearrangements compared to bread wheat

The Plant Genome, Journal Year: 2025, Volume and Issue: 18(1)

Published: Feb. 26, 2025

Abstract Goatgrasses with U‐ and M‐genomes are important sources of new alleles for wheat breeding to maintain yield quality under extreme conditions. However, the introgression beneficial traits from wild Aegilops species into has been limited by poor knowledge their genomes scarcity molecular tools. Here, we present first linkage map allotetraploid biuncialis Vis., developed using 224 F 2 individuals derived a cross between MvGB382 MvGB642 accessions. The comprises 5663 DArTseq markers assigned 15 groups corresponding 13 chromosomes. Chromosome 1M b could not be constructed due lack recombination caused rearrangements in accession. genetic spans 2518 cM an average marker density 2.79 cM. skeleton contains 920 segregating markers, divided M sub‐genome (425 markers) U (495 markers). Chromosomes sub‐genome, originating comosa Sm. Sibth. et Sm., show well‐preserved collinearity Triticum aestivum L. In contrast, chromosomes umbellulata Zhuk., exhibit varying degree collinearity, 1U , 3U 5U retaining substantial level while 2U 4U 6U 7U significant rearrangements. A quantitative trait locus affecting fertility was identified near centromere on long arm chromosome 3M explaining 23.5% variance. genome structure highlighted map, provides insights speciation within will support alien gene transfer wheat.

Language: Английский

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Progress and innovations of gene cloning in wheat and its close relatives

Theoretical and Applied Genetics, Journal Year: 2025, Volume and Issue: 138(5)

Published: April 29, 2025

Abstract Key message Wheat and its close relatives have large complex genomes, making gene cloning difficult. Nevertheless, developments in genomics over the past decade made it more feasible. The genomes of cereals, especially bread wheat, always been a challenge for mapping cloning. recent advances led to significant progress this field. Currently, high-quality reference sequences are available major wheat species their relatives. New high-throughput genotyping platforms next-generation sequencing technologies combined with genome complexity reduction techniques mutagenesis opened new avenues In review, we provide comprehensive overview genes cloned so far discuss strategies used these genes. We highlight advantages drawbacks individual approaches show how particular genomic contributed A wide range resources increase number successful projects decade, demonstrating that is now feasible perform rapid agronomically important genes, even as wheat.

Language: Английский

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Chromosomal Location of Pm12—A Novel Powdery Mildew Resistance Gene from Avena sterilis

Genes, Journal Year: 2022, Volume and Issue: 13(12), P. 2409 - 2409

Published: Dec. 19, 2022

Identification of new, effective disease resistance genes is a very important aspect plant breeding. Also the precise location individual loci and tagging them with DNA markers for marker assisted selection. The aim present study was identification molecular linked Pm12, new gene to powdery mildew, their in oat genome. analysis performed on 167 F2 individuals from hybrid Fuchs × CN67383, status locus each verified by progeny test F3. Segregation ratios confirmed monogenic nature resistance. Making use sequence data OT3098 v2 genome reference assembly, Pm12 located chromosome 7C. A comparison also made consensus map, which there are more reports mapped date. mapping results suggest that interval 103.8-111.7 cM this map. No mildew has been identified region so far, suggesting Avena sterilis CN67383 carries novel offering information included annotation allowed candidate close cluster Pm12. This may provide an interesting source further pathways various response stress infection.

Language: Английский

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Genome-wide association study for resistances to yellow rust, powdery mildew, and Septoria tritici blotch in cultivated emmer

Euphytica, Journal Year: 2024, Volume and Issue: 220(3)

Published: Feb. 21, 2024

Abstract Emmer is a progenitor of bread wheat and evolved in the Levant together with yellow rust (YR), powdery mildew (PM) fungi, precursor Zymoseptoria tritici causing Septoria blotch (STB). We performed genome-wide association mapping for three disease resistances 143 cultivated emmer accessions multi-environmental trials. Significant (P < 0.001) genotypic variation was found high heritabilities to two biotrophs moderate heritability STB resistance. For YR, PM, severity nine, three, seven marker-trait associations, respectively, were detected that significant across all environments. Most them low effect, but PM resistance potentially new major gene on chromosome 7AS. Genomic prediction abilities throughout (≥ 0.8) decreased only slightly YR when done second year first as training set 0.7). ability much lower this scenario (0.4). Despite this, genomic selection should be advantageous given large number small QTLs responsible quantitative resistances. A challenge future combine these multiple better lodging tolerance higher grain yield.

Language: Английский

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Potential of tetraploid wheats in plant breeding: A review

Plant Science, Journal Year: 2024, Volume and Issue: 346, P. 112155 - 112155

Published: June 15, 2024

Language: Английский

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