Engineered Phomopsis liquidambaris with Fhb1 and Fhb7 Enhances Resistance to Fusarium graminearum in Wheat

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(3), P. 1391 - 1404

Published: Jan. 10, 2023

Fusarium head blight is one of the most serious diseases caused by graminearum in wheat. Here, we developed a new way to prevent and control introducing resistance genes Fhb1 Fhb7 into endophytic fungus Phomopsis liquidambaris, named PL-Fhb1 PL-Fhb7, respectively, which could colonize The wheat seedlings were preinoculated with PL-Fhb7 enhance against deoxynivalenol (DON) inhibited growth F. 73% 49%, respectively. incidence rate diseased spikes decreased 35.2% 45.4%, corresponding DON levels for grains from 13.2 1.79 μg/g 0.39 when leaves after overwintering, rates 25.7% 34.7%, 17.48 1.23 0 flowers inoculated subsequently infected graminearum, It was confirmed that transformed DON-glutathione (GSH) using high-performance liquid chromatography-mass spectrometry (HPLC-MS). However, may have increased plant immunity enhanced graminearum. This study indicates engineered endophytes can improve presents method biological blight.

Language: Английский

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Comparative genomics-based insights into Xanthomonas indica , a non-pathogenic species of healthy rice microbiome with bioprotection function

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: 90(9)

Published: Aug. 19, 2024

ABSTRACT Xanthomonas species are major pathogens of plants and have been studied extensively. There is increasing recognition the importance non-pathogenic within same genus. With this came need to understand genomic functional diversity (NPX) at strain level. This study reports isolation investigation into variation in NPX isolates, chiefly indica , a newly discovered from rice. The establishes relationship X. strains clade I Xanthomonads with another species, sontii also associated rice seeds. Identification highly diverse strains, open-pan genome, systematic hyper-variation lipopolysaccharide biosynthetic locus when compared pathogenic indicates acquisition new functions for adaptation. Furthermore, comparative genomics studies established absence virulence genes such as type III secretion system effectors, which present pathogens, presence known bacterial-killing IV (X-T4SS). were found protect bacterial leaf blight pathogen, oryzae pv. (Xoo). phenotype an X-T4SS mutant suggests redundancy genetic basis mechanisms involved bioprotection function, may include multiple loci, putative bacteriocin-encoding gene clusters involvement other factors nutrient niche competition apart induction innate immunity through shared microbial-associated molecular patterns. rice-NPX community its counterpart can be promising model understanding plant-microbe-microbiome interaction studies. IMPORTANCE group bacteria characteristic lifestyle phytopathogen. However, discovery shift bacteria. Multi-strain, in-depth genomic, evolutionary on each these still lacking. provides novel insights genome diversity, dynamics, trends microbiome relatives that form sub-clade. Interestingly, we uncovered species. plant protection property shows their part healthy microbiome. finding open pan-genome large-scale significant role host findings high-quality resources will allow further host-associated microbial health.

Language: Английский

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Isolation and optimization of Bacillus thuringiensis BRB-3 isolated from germinated black upland rice seeds for GABA production

Heliyon, Journal Year: 2025, Volume and Issue: 11(3), P. e41884 - e41884

Published: Jan. 11, 2025

Gamma-aminobutyric acid (GABA) is a non-coded amino that recently gained increased attention within the functional food industry. This study aimed to isolate endophytic bacteria from sterilized surfaces of germinated black upland rice seeds for GABA production and optimization. A total 38 isolates were screened glutamic decarboxylase (GAD) activity using colorimetric method. Among these, thirteen exhibited positive GAD activity. The BRB-3 strain emerged as most promising, producing highest levels (4.54 g/L) at an optimal pH 6.5. However, was sensitive lower than 5.5; so adjusted 6.5 through all experiments. Further identification performed 16S rRNA gene sequence, which placed it in Bacillus cereus group. Subsequent rpoB sequence analysis confirmed thuringiensis BRB-3. optimized MRS broth by investigating carbon, nitrogen, monosodium glutamate (MSG), temperature. Interestingly, concentration MSG did not impact B. Under conditions 3 % soy protein nitrogen source, 2 sucrose, no added 30 °C, 12 days, achieved high yield 25.06 g/L (242.9 mM). Therefore, demonstrated significant potential could be utilized various products, including seeds.

Language: Английский

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The Seed Microbiome: Microbial Hashes for Plant Wellbeing

Open Journal of Environmental Biology, Journal Year: 2025, Volume and Issue: 10(1), P. 007 - 022

Published: Jan. 1, 2025

Seeds harbour diverse and unique microbial communities that significantly influence seed quality, germination, seedling health, overall plant resilience. The microbiome is shaped by both vertical transmission (from parent plants) horizontal the surrounding environment), with colonization highly dependent on floral pathways, composition, structure. However, mechanisms governing transmission, their functional roles, impact development remain poorly understood. This gap in knowledge limits ability to harness beneficial interactions for improving germination success health. review explores sources routes of microbiome, emphasizing its role nutrition, disease resistance, stress tolerance. Additionally, it highlights dynamic establishment during long-term health longevity. Understanding complex within crucial leveraging potential sustainable agriculture. By uncovering these relationships, future research can pave way microbiome-based strategies enhance crop productivity, resilience, ecological sustainability.

Language: Английский

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Assembly and application of a synthetic bacterial community for enhancing barley tolerance to drought

Frontiers in Bacteriology, Journal Year: 2025, Volume and Issue: 4

Published: April 25, 2025

Under climate change scenarios for temperate regions in Europe, prolonged droughts pose a major threat to barley production, but few studies have been conducted on stress mitigation strategies using plant-beneficial rhizobacteria. With this mind, we isolated and screened culture collection of drought-tolerant bacteria from the rhizosphere. From collection, assembled 16-member consortium based their relative abundances rhizosphere after drought vitro osmotic tolerance (Drought Tolerant Synthetic microbial Community/”DT-SynCom”). Members DT-SynCom range Proteobacteria Firmicutes Actinobacteria. We used Oxford Nanopore Illumina technologies assemble complete genomes. Whole genome annotation revealed presence number genes associated with plant growth promoting traits such as IAA biosynthesis, ACC deaminase activity siderophore production. In assays confirmed auxin activity, inorganic P solubilization, cellulase chitinase activities by selected bacterial strains. The members were not antagonistic each other, either neutral or beneficial shoot root when applied individually . To clarify effect designed tolerance, pot experiment was under conditions. reduced wilting leaves had positive drought. results research suggest that result improved stress.

Language: Английский

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Seed Endophytic Bacteria from Manilkara zapota L. and Their Influence as Rice Seed Priming Agents

Current Microbiology, Journal Year: 2025, Volume and Issue: 82(6)

Published: May 3, 2025

Language: Английский

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Interaction studies of Serendipita indica and Zhihengliuella sp. ISTPL4 and their synergistic role in growth promotion in rice

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: May 24, 2023

Rapid urbanization and globalization demand increasing agricultural productivity. Soil nutrient supply capacity is continuously decreasing due to soil erosion, degradation, salt deposition, undesired element, metal water scarcity, an uneven delivery system. Rice cultivation requires a large amount of which becoming detrimental these activities. There need increase its Microbial inoculants are increasingly important in achieving sustainable production systems. The current study was conducted investigate the interaction between root endophytic fungus Serendipita indica (S. indica) actinobacterium Zhihengliuella sp. ISTPL4 (Z. ISTPL4) their synergistic effects on growth rice (Oryza sativa L). Both S. Z. showed positive interactions. Growth observed at different days after inoculation, stimulated when inoculated 5 dafi (days fungal inoculation). promoted as it increased spore germination. Furthermore, confocal scanning electron microscopy (SEM) analyses 27% size presence ISTPL4. In liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis alanine glutamic acid sequential co-culture compared individual cultures. Sequential inoculation significantly biochemical physical characteristics inoculum. Biochemical parameters such chlorophyll content, total soluble sugar, flavonoid content by up 57%, 47%, 39%, respectively, combined inoculum This will be first study, best our knowledge, shows roles promotion rice. this novel combination can also used boost other crops yield.

Language: Английский

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Seed bacterial microbiota in post-submergence tolerant and sensitive barley genotypes

Functional Plant Biology, Journal Year: 2024, Volume and Issue: 51(2)

Published: Jan. 24, 2024

Flooding is a predominant abiotic stress for cultivated plants, including barley. This cereal crop shows large adaptability to different environmental conditions, suggesting the presence of key traits tolerate adverse conditions. During germination, genetic variations account dissimilarities in flooding tolerance. However, differences seed microbiota may also contribute tolerance/sensitivity during seedling establishment. work investigated microbiome among grains barley accessions. Two phenotypes were compared, each either tolerant or sensitive short submergence period followed by recovery. The study used metataxonomic analysis based on 16S ribosomal RNA gene sequencing and subsequent functional prediction. Our results support hypothesis that bacterial inhabiting seeds are between accessions, which harbour specific phyla families. Finally, bacteria detected accessions show peculiar enrichment suggests possible connection with successful germination

Language: Английский

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Xanthomonas sontii, and not Xanthomonas sacchari, is the predominant, vertically transmitted core rice seed endophyte

Phytopathology, Journal Year: 2024, Volume and Issue: 114(9), P. 2017 - 2023

Published: June 25, 2024

Seed endophytes, particularly the abundant, core, and vertically transmitted species, are major areas of focus in host microbiome studies. Apart from being first members to colonize, they accompany plant throughout its development stages next generation. Recently published studies have reported keystone species be Xanthomonas sacchari, a core endophyte that is rice with probiotic properties. Furthermore, was involved assembly beneficial bacteria after early inoculation seeds. However, strains discussed these were misclassified as X. well-known pathogen sugarcane. By including nonpathogenic plant-protective functions seeds, we correctly established phylogenetic taxonomic identity sontii. This will enable researchers use correct reference or lab strain sontii for further systematic in-depth model plant–microbe interactions apart exploitation seed health.

Language: Английский

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Amplicon Sequencing Analysis of Submerged Plant Microbiome Diversity and Screening for ACC Deaminase Production by Microbes

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13330 - 13330

Published: Dec. 12, 2024

Submerged plants can thrive entirely underwater, playing a crucial role in maintaining water quality, supporting aquatic organisms, and enhancing sediment stability. However, they face multiple challenges, including reduced light availability, fluctuating conditions, limited nutrient access. Despite these stresses, submerged demonstrate remarkable resilience through physiological biochemical adaptations. Additionally, their interactions with microbial communities are increasingly recognized as pivotal mitigating environmental stresses. Understanding the diversity of is for comprehending complex between environments. This research aims to identify screen microbes from plant samples capable producing 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase explore metagenomic analysis. Microbes were isolated screened ACC production, techniques, co-occurrence network analysis, used examine within communities. deaminase-producing significantly enhance metabolism under stress conditions. The identification culturable bacteria revealed that most belong genera Pseudomonas, Bacillus, Acinetobacter. A total 177 strains cultured, molecular revealing 79 reductant, 86 non-reductant, 12 uncultured strains. Among 162 activity, 50 tested positive. To further understand dynamics, collected both natural sources artificial pond reservoirs assess impact location on flood-associated microbiomes plants. Metagenomic analysis was conducted epiphytic endophytic samples. By exploring overall composition dynamics associated plants, this seeks deepen our understanding plant–microbe screening helped diverse activity amplicon sequencing paved way towards identifying shaping microbiome microbes. Co-occurrence highlighted intricate Notably, observed plant-associated across different locations, distinct variations populations identified

Language: Английский

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