Rust
diseases,
caused
by
fungal
pathogens
pose
a
substantial
threat
to
global
wheat
production
including
India.
The
2NvS
translocation
carrying
Lr37-Sr38-Yr17
from
Aegilops
ventricosa
has
been
pivotal
in
developing
disease-resistant
wheat,
hosting
essential
resistance
genes
against
various
diseases.
Recent
research
suggests
its
potential
effectiveness
the
globally
emerging
of
blast.
Initially,
conventional
backcross
breeding
efforts
were
attempted
integrate
this
gene
cluster
into
ten
adapted
Indian
bread
cultivars.
Extensive
phenotyping
populations
at
seedling
and
adult
stages
led
creation
rust-resistant
BC7F8
near
isogenic
lines
(NILs)
with
desirable
traits.
However,
approach
proved
time-consuming
relied
on
disease
pressure,
ultimately
resulting
these
no
longer
being
cultivation.
Subsequently,
marker-assisted
employing
VENTRIUP/LN2
molecular
marker
was
employed
confirm
presence
facilitate
transfer
modern
varieties,
notably
HD
2733,
PBW
343,
DBW39,
2967
HW
2045
particularly
targeting
North
Eastern
Plain
Zone
combat
blast
using
previously
developed
as
donor
BC3F6
generation.
integration
via
method
limited
represents
significant
stride
rust
breeding,
offering
promising
bolster
food
security
fostering
development
varieties.
Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 11, 2024
Abstract
Background
Wild
emmer
wheat
(WEW)
(Triticum
turgidum
subsp.
dicoccoides)
is
one
of
the
important
crops
domesticated
in
Neolithic
revolution
that
shifted
humanity
to
agrarian
societies.
Leveraging
whole-genome
sequencing
(WGS)
data
from
291
accessions
at
~
9.5x
coverage,
we
identified
3.4
million
high-quality
SNP
markers
and
utilized
them
for
population
genetics
evolutionary
studies.
We
also
conducted
Genome-wide
association
studies
(GWAS)
seedling-stage
resistance
five
races
stem,
leaf,
stripe/yellow
rust
pathogens.
investigated
candidate
genes
using
ortholog
sequence
alignment.
Results
Phylogenetic
clustering,
principal
component
analysis,
structure
assessment
revealed
distinct
genetic
subgroups
within
WEW,
specifically
Northern
Population,
Southern
Levant
(SL)
population,
a
highly
subgroup
near
Sea
Galilee,
known
as
race
judaicum.
Diversity
pairwise
FST
analyses
highlighted
varying
levels
diversity
distances
among
these
subpopulations.
The
SL
exhibited
higher
all
three
pathogens
compared
judaicum
populations.
GWAS
28,
25,
32
significant
loci
associated
with
stripe
resistance,
respectively.
Major
explained
up
60%
phenotypic
variation
some
were
linked
multiple
novel.
Accessions
such
TA11204,
TA1041,
TA1047,
TA11196,
TA77,
TA93,
TA1065,
TA66
demonstrated
broad
races.
Conclusions
In
summary,
this
study
provides
WEW
germplasms
guides
future
research
on
selection
introgression
into
cultivated
bread
durum
wheat,
contributing
stable
production
food
crops.
Rust
diseases,
caused
by
fungal
pathogens
pose
a
substantial
threat
to
global
wheat
production
including
India.
The
2NvS
translocation
carrying
Lr37-Sr38-Yr17
from
Aegilops
ventricosa
has
been
pivotal
in
developing
disease-resistant
wheat,
hosting
essential
resistance
genes
against
various
diseases.
Recent
research
suggests
its
potential
effectiveness
the
globally
emerging
of
blast.
Initially,
conventional
backcross
breeding
efforts
were
attempted
integrate
this
gene
cluster
into
ten
adapted
Indian
bread
cultivars.
Extensive
phenotyping
populations
at
seedling
and
adult
stages
led
creation
rust-resistant
BC7F8
near
isogenic
lines
(NILs)
with
desirable
traits.
However,
approach
proved
time-consuming
relied
on
disease
pressure,
ultimately
resulting
these
no
longer
being
cultivation.
Subsequently,
marker-assisted
employing
VENTRIUP/LN2
molecular
marker
was
employed
confirm
presence
facilitate
transfer
modern
varieties,
notably
HD
2733,
PBW
343,
DBW39,
2967
HW
2045
particularly
targeting
North
Eastern
Plain
Zone
combat
blast
using
previously
developed
as
donor
BC3F6
generation.
integration
via
method
limited
represents
significant
stride
rust
breeding,
offering
promising
bolster
food
security
fostering
development
varieties.
