Comparative Transcriptome Analysis of Emerging Young and Mature Leaves of Bienertia sinuspersici, a Single-Cell C4 Plant DOI Creative Commons
Richard M. Sharpe, Seanna Hewitt, Gerald E. Edwards

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 26, 2023

Abstract Efficient carbon capture by plants is crucial to meet the increasing demands for food, fiber, feed, and fuel worldwide. One potential strategy improve photosynthetic performance of conversion C3-type crops C4-type crops, enabling them perform photosynthesis at higher temperatures with less water. such as corn, possess a distinct Kranz anatomy, where occurs in two cell types. Remarkably, Bienertia sinuspersici one four known land plant species C4 within single cell, characterized dimorphic chloroplasts corresponding intracellular biochemistry. The young emerging leaves exhibit C3 anatomy which differentiate into unique mature. A comparative transcriptome analysis yielded total 72,820 transcripts 72,253 mature B. . In leaf, enrichment processes associated cycle, cellular developmental patterning, transcriptional regulatory mechanisms was observed. leaf displayed photosynthesis, chloroplast components, translational post-translational modifications. Notably, several transcription factors auxin response factor (ARF), basic helix-loop-helix (bHLH), GATA, homeodomain (HD), MYB, NAC, squamosa promoter-binding protein-like (SPL), zinc finger (ZF) family were differentially expressed leaf. These data expand our insights molecular basis ’s compartmentalization, dimorphism, single-cell biochemistry, information can be useful ongoing efforts transform C-4 type.

Language: Английский

Comparative transcriptome analysis of emerging young and mature leaves of Bienertia sinuspersici, a single-cell C4 plant DOI Creative Commons
Richard M. Sharpe, Seanna Hewitt, Gerald E. Edwards

et al.

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e19282 - e19282

Published: April 29, 2025

Efficient carbon capture by plants is crucial to meet the increasing demands for food, fiber, feed, and fuel worldwide. One potential strategy improve photosynthetic performance of conversion C3-type crops C4-type crops, enabling them perform photosynthesis at higher temperatures with less water. such as corn, possess a distinct Kranz anatomy, where occurs in two cell types. Remarkably, Bienertia sinuspersici one four known land plant species that C4 within single cell. This unique single-cell (SCC4) anatomy characterized dimorphic chloroplasts corresponding intracellular biochemistry. Because young, emergent leaves first exhibit C3anatomy then differentiate into mature, represents an excellent system explore basis C3 transition. To gain insight genes pathways associated transition between emerging young mature leaves, comparative transcriptome analysis was conducted which global gene expression ontologies were compared stages. In leaf, differentially expressed enrichment cycle cytoskeletal dynamics observed, while leaf displayed processes cellular energetics. Additionally, numerous transcription factors (TFs) metabolic homeostasis, hormone stress signaling, developmental regulation throughout development, TF profiles each stage. These data expand our insights molecular Binertia's compartmentalization, chloroplast dimorphism, biochemistry provide information will be useful ongoing efforts transform type.

Language: Английский

Citations

0
Effect of Hydrogen Peroxide Application on Salt Stress Mitigation in Bell Pepper (Capsicum annuum L.) DOI Creative Commons
Jéssica Aragão, Geovani Soares de Lima, Vera Lúcia Antunes de Lima

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(16), P. 2981 - 2981

Published: Aug. 18, 2023

The present study aimed to evaluate the effects of foliar application hydrogen peroxide on attenuation salt stress growth, photochemical efficiency, production and water use efficiency ‘All Big’ bell pepper plants. experiment was conducted under greenhouse conditions in Campina Grande, PB, Brazil. Treatments were distributed a randomized block design, 5 × factorial scheme, corresponding five levels electrical conductivity irrigation (0.8, 1.2, 2.0, 2.6 3.2 dS m−1) concentrations (0, 15, 30, 45 60 μM), with three replicates. Foliar at concentration 15 μM attenuated deleterious biomass accumulation components plants irrigated using an up m−1. spraying intensified stress. classified as moderately sensitive stress, salinity threshold 1.43 m−1 unit decrease 8.25% above this level.

Language: Английский

Citations

7
Genome-wide identification of a novel Na+ transporter from Bienertia sinuspersici and overexpression of BsHKT1;2 improved salt tolerance in Brassica rapa DOI Creative Commons
Vadivelmurugan Irulappan, Hyun Woo Park, Sang-Yun Han

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Dec. 12, 2023

Salt stress is an ever-increasing stressor that affects both plants and humans. Therefore, developing strategies to limit the undesirable effects of salt essential. Sodium ion exclusion well known for its efficient salt-tolerance mechanism. The High-affinity K + Transporter (HKT) excludes excess Na from transpiration stream. This study identified characterized HKT protein family in Bienertia sinuspersici , a single-cell C 4 plant. Overly Sensitive 1 (SOS1) expression levels were examined B. Arabidopsis thaliana leaves under four different conditions: 0, 100, 200, 300 mM NaCl. Furthermore, BsHKT1;2 was cloned, thereby producing stable transgenic Brassica rapa . Our results showed that, compared A. as glycophyte, expanded halophyte with three paralogs. phylogenetic analysis revealed paralogs belonging subfamily I. Out copies, higher control conditions than Stable overexpressing 35S::BsHKT1;2 tolerance non-transgenic plants. Higher biomass longer roots observed demonstrates evolutionary functional differences proteins between glycophytes halophytes associates role imparting productivity.

Language: Английский

Citations

4
Phytochemical analysis of Bienertia sinuspersici extract and its antioxidant and antimicrobial activities DOI Creative Commons

Helmy A. Aamer,

Abdulaziz A. Al–Askar, Rania El-Tanbouly

et al.

Open Agriculture, Journal Year: 2024, Volume and Issue: 9(1)

Published: Jan. 1, 2024

Abstract Natural products derived from plants are emerging as a valuable resource for range of antimicrobial agents in sustainable agriculture. The current work looks the first time at properties methanolic extract Bienertia sinuspersici against phytopathogenic microorganisms vitro , using disk diffusion and food poisoning techniques. phytochemical analysis revealed presence total phenols, flavonoids, antioxidant activity measured 34.2, 20.6, 20.1 mg/g, respectively. Furthermore, concentrations tannins, saponins, alkaloids were documented 17.6, 9.8, 3.2 high-performance liquid chromatography examination polyphenolic chemicals that gallic acid was most abundant compound 2.22 whereas methyl gallate least 0.021 mg/g. Gas chromatography–mass spectrometry primary components oleic acid, n -hexadecanoic ester 11-octadecenoic acid. had maximal inhibitory percentage 57.8% Fusarium oxysporum 300 µg/mL, 60.4% Rhizoctonia solani 500 88.2% Botrytis cinerea . On other hand, demonstrated inhibition Pectobacterium atrosepticum growth by 10.67 mm concentration 100 while Ralstonia solanacearum inhibited 9.76 carotovorum 9 mm. Overall, B. promising efficacy antibacterial antifungal different plant pathogens. Therefore, it could offer environmentally friendly alternative managing diseases.

Language: Английский

Citations

0
Comparative Transcriptome Analysis of Emerging Young and Mature Leaves of Bienertia sinuspersici, a Single-Cell C4 Plant DOI Creative Commons
Richard M. Sharpe, Seanna Hewitt, Gerald E. Edwards

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 26, 2023

Abstract Efficient carbon capture by plants is crucial to meet the increasing demands for food, fiber, feed, and fuel worldwide. One potential strategy improve photosynthetic performance of conversion C3-type crops C4-type crops, enabling them perform photosynthesis at higher temperatures with less water. such as corn, possess a distinct Kranz anatomy, where occurs in two cell types. Remarkably, Bienertia sinuspersici one four known land plant species C4 within single cell, characterized dimorphic chloroplasts corresponding intracellular biochemistry. The young emerging leaves exhibit C3 anatomy which differentiate into unique mature. A comparative transcriptome analysis yielded total 72,820 transcripts 72,253 mature B. . In leaf, enrichment processes associated cycle, cellular developmental patterning, transcriptional regulatory mechanisms was observed. leaf displayed photosynthesis, chloroplast components, translational post-translational modifications. Notably, several transcription factors auxin response factor (ARF), basic helix-loop-helix (bHLH), GATA, homeodomain (HD), MYB, NAC, squamosa promoter-binding protein-like (SPL), zinc finger (ZF) family were differentially expressed leaf. These data expand our insights molecular basis ’s compartmentalization, dimorphism, single-cell biochemistry, information can be useful ongoing efforts transform C-4 type.

Language: Английский

Citations

0