Mitigation of salt stress in lettuce by a biostimulant that protects the root absorption zone and improves biochemical responses

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 16, 2024

Horticultural crops constantly face abiotic stress factors such as salinity, which have intensified in recent years due to accelerated climate change, significantly affecting their yields and profitability. Under these conditions, it has become necessary implement effective sustainable solutions guarantee agricultural productivity food security. The influence of BALOX ® , a biostimulant plant origin, was tested on the responses salinity Lactuca sativa L. var. longifolia plants exposed salt concentrations up 150 mM NaCl, evaluating different biometric biochemical properties after 25 days treatment. Control were cultivated under same conditions but without An situ analysis root characteristics using non-destructive, real-time method also performed. treatments inhibited growth, reduced chlorophyll carotenoid contents, increased Na + Cl - roots leaves while reducing those Ca 2+ . application had positive effect because stimulated growth level photosynthetic pigments. In addition, content presence absence salt. salt-induced accumulation biomarkers, proline, malondialdehyde (MDA), hydrogen peroxide (H 2 O ). Therefore, appears reduce osmotic, ionic oxidative levels salt-treated plants. Furthermore, treatments’ biostimulant’s direct effects indicated that ’s primary mechanism action probably involves improving nutrition, even severe by protecting stimulating absorption zone.

Language: Английский

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Unravelling the biostimulant activity of a protein hydrolysate in lettuce plants under optimal and low N availability: a multi‐omics approach

Physiologia Plantarum, Journal Year: 2024, Volume and Issue: 176(3)

Published: May 1, 2024

Abstract The application of protein hydrolysates (PH) biostimulants is considered a promising approach to promote crop growth and resilience against abiotic stresses. Nevertheless, PHs bioactivity depends on both the raw material used for their preparation molecular fraction applied. present research aimed at investigating mechanisms triggered by applying PH its fractions plants subjected nitrogen limitations. To this objective, an integrated transcriptomic‐metabolomic was assess lettuce grown under different levels treated with either commercial Vegamin® or PH1(>10 kDa), PH2 (1–10 kDa) PH3 (<1 kDa). Regardless provision, biostimulant enhanced biomass, likely through hormone‐like activity. This confirmed modulation genes involved in auxin cytokinin synthesis, mirrored increase metabolic these hormones. Consistently, upregulated cell wall plasticity. Furthermore, accumulation specific metabolites suggested activation multifaceted antioxidant machinery. Notwithstanding, stress‐response transcription factors detoxification processes observed. coordinated action entities might underpin increased nitrogen‐limiting conditions. In conclusion, integrating omics techniques allowed elucidation mechanistic aspects underlying crops. Most importantly, comparison showed that, except few peculiarities, effects induced were equivalent, suggesting that highest ascribable lightest fraction.

Language: Английский

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Integrated multi-omic approach reveals the effect of a Graminaceae-derived biostimulant and its lighter fraction on salt-stressed lettuce plants

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: May 10, 2024

Abstract Plant biostimulants are widely applied in agriculture for their ability to improve plant fitness. In the present work, impact of Graminaceae -derived protein hydrolysate (P) and its lighter molecular fraction F3 (< 1 kDa) on lettuce plants, subjected either no salt or high conditions, was investigated through combination metabolomics transcriptomics. The results showed that both treatments significantly modulated transcriptome metabolome plants under salinity stress, highlighting an induction hormonal response. Nevertheless, P also displayed several peculiarities. specifically response ethylene MAPK signaling pathway, whereas treatment induced a down-accumulation secondary metabolites, albeit genes controlling biosynthesis osmoprotectants antioxidants were up-regulated. Moreover, according with auxin modulation, promoted cell wall biogenesis plasticity salt-stressed plants. Notably, our data outlined epigenetic control gene expression by treatment. Contrarily, experimental just partially agreement when not stressed treated F3, considered. Indeed, reduced accumulation metabolites analyses hormone pathways modulation would suggest preferential allocation resources towards growth, is coherent down-regulation photosynthetic machinery, CO 2 assimilation rate leaves biomass. conclusion, demonstrate that, although they might activate different mechanisms, can result similar benefits, as far protective osmolytes enhanced tolerance oxidative stress concerned. exhibits slightly greater growth promotion effects conditions. Most importantly, this research further corroborates biostimulants’ mode action dependent plants’ physiological status composition, underscoring importance investigating bioactivity components design tailored applications agricultural practice.

Language: Английский

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Joint Action of Trichoderma atroviride and a Vegetal Derived-Protein Hydrolysate Improves Performances of Woodland Strawberry in Italy

Horticulturae, Journal Year: 2024, Volume and Issue: 10(5), P. 459 - 459

Published: April 30, 2024

Woodland strawberry (Fragaria vesca L.) is an underutilized plant species that could benefit from the use of biostimulants to improve crop productivity and fruit quality. The scope present study was appraise influence two (Trichoderma atroviride protein hydrolysate)—used either alone or combined—on performance economic profitability woodland genotypes (‘Alpine’ ‘Regina delle Valli’). Overall, data showed ‘Alpine’ had highest productive performances, whereas Valli’ revealed qualitative traits. T. inoculation V-PH application significantly boosted marketable yield (+20.5% for +12.9% V-PH), total sugars (+1.9% Trichoderma +1.4% V-PH) anthocyanins (+14.1% +9.8% compared non-treated plants. Plants supplied with both a higher (+34.8%), mean weight (+6.0%), dry matter (+13.8%), (+3.5%), ascorbic acid (+12.7%), flavonoid (+26.3%) (+29.9%) Furthermore, our polyphenol concentration recorded in treated combination sprayed V-PH, antioxidant activity found when plants were biostimulants. Our pointed out microbial non-microbial biostimulants, especially combination, might be useful strategy improving performances and, therefore, encouraging their cultivation, valorization (+6208.3 EUR /ha exposed biostimulants).

Language: Английский

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Abiotic Stress and Mechanisms of Stress Tolerance in Vegetable Crops

Published: Jan. 1, 2025

Language: Английский

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Integrative transcriptomic and physiological analyses uncover mechanisms by which arbuscular mycorrhizal fungi mitigate salt stress in sugar beet

Mycorrhiza, Journal Year: 2025, Volume and Issue: 35(3)

Published: April 28, 2025

Language: Английский

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Joint Action of Trichoderma atroviride and a Vegetal Derived-Protein Hydrolysate Improves Yield, Fruit Quality and Economic Profitability of Two Woodland Strawberry Cultivars Grown Under Greenhouse

Published: March 19, 2024

Woodland strawberry (Fragaria vesca L.) is an underutilized plant species, appreciated by consumers for its qualitative features. Utilizing technical means of production, such as biostimulants, that can increase crop productivity and quality could be highly important in woodland cultivation. The scope the present study was to appraise influence two biostimulants (Trichoderma atroviride protein hydrolysate) - used either alone or combined on performance economic profitability genotypes (‘Alpine’ ‘Regina delle Valli’). Overall, data showed ‘Alpine’ had highest productive performances, whereas Valli’ revealed fruit traits. T. inoculation V-PH application significantly boosted marketable yield (+20.5% +12.9% V-PH), total sugars (+1.9% Trichoderma +1.4% V-PH) anthocyanins (+14.1% +9.8% compared non-treated plants. Plants supplied with both higher (+34.8%), mean weight (+6.0%), dry matter (+13.8%), (+3.5%), ascorbic acid (+12.7%), flavonoid (+26.3%) (+29.9%) Furthermore, our polyphenols concentration recorded treated combination sprayed V-PH, antioxidant activity found when plants were biostimulants. Our pointed out microbial non-microbial especially combination, might useful strategy improving performances species and, therefore, encourage cultivation, valorisation (+10195.3 €/ha exposed biostimulants).

Language: Английский

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Salinity stress mitigation by radicular and foliar humic substances application in lettuce plants

Plant Growth Regulation, Journal Year: 2024, Volume and Issue: 104(1), P. 151 - 167

Published: May 15, 2024

Abstract Salinity stress constitutes one of the main abiotic stresses that considerably reduces crop yield. An approach to enhance plant growth under salt involves addition humic substances (HS) roots or leaves. Here, we evaluated potential use BLACKJAK®, an HS-based product, tolerance in lettuce ( Lactuca sativa L.). For this aim, plants were exposed salinity (100 mM NaCl), + HS: radicular (R)-HS (0.40 mL/L and 0.60 mL/L) foliar (F)-HS (7.50 10.00 mL/L), along with a control (without NaCl). Parameters related growth, Na K accumulation, photosynthetic activity, oxidative stress, enzymatic non-enzymatic antioxidants, as well proline levels evaluated. Results showed R F-HS enhanced tolerance. In addition, offered greater improvement relation shoot fresh weight, relative rate, area, being best dose. This could be associated reduced translocation decreasing /K ratio. Furthermore, HS improved net Rubisco carboxylation efficiency, photosystem II performance, ROS lipid peroxidation. Hence, our data show BLACKJAK® improve salinity, application slightly better than achieve especially at

Language: Английский

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Plant protein hydrolysate and arbuscular mycorrhizal fungi synergistically orchestrate eggplant tolerance to iodine supply: A two-year study

Scientia Horticulturae, Journal Year: 2024, Volume and Issue: 336, P. 113437 - 113437

Published: June 28, 2024

Biofortification is a promising strategy to overcome iodine (I) deficiencies in the world population. However, since not essential for plants, its administration may cause phytotoxicity issues. The objective of this work was evaluate effects two biostimulants [arbuscular mycorrhizal fungi (AM) and plant protein hydrolysates (PH)], used alone or combination, on eggplant plants treated with three different I doses (0, 300 600 mg L−1). Results underlined that highest dosage significantly reduced growth, yield, fruit dry matter content, chlorophylls stomatal conductance compared control. Whereas, increased total anthocyanins, chlorogenic acid, antioxidant activity, concentration, proline, H2O2 malondialdehyde biofortified non-biofortified plants. enhanced growth chlorophylls, anthocyanins acid control, especially when both were supplied. Interestingly, it often recorded an interaction between biostimulants, suggesting PH AM had buffer effect toxicity, specifically combined (PH+AM). Overall, our study pointed out mutual use microbial non-microbial (PH) application L−1 might be helpful approach relieve detrimental high dosages and, simultaneously, increase crop yield quality eggplant.

Language: Английский

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Biosynthesis and Extraction of Chlorophyll, Carotenoids, Anthocyanins, and Betalaine In Vivo and In Vitro

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(9), P. 10662 - 10676

Published: Sept. 23, 2024

As natural bioactive compounds, plant pigments play crucial roles not only in phenotype, growth, development, and adaptation to stress but also hold unique value biotechnology, healthcare, industrial applications. There is growing interest the biosynthesis acquisition of pigments. Thus, this paper explores emerging extraction methods elucidates pathways four key pigments, chlorophylls, carotenoids, anthocyanins, betalaine vivo vitro. We comprehensively discuss application solvent, supercritical fluid [extraction], ultrasonic, microwave-assisted techniques, as well introducing enzymes, precursors, synthetic involved pigment synthesis. δ-Aminolevulinic acid represents a pivotal initiating enzyme for chlorophyll synthesis, whereas isopentenylpyrophosphate, (IPP) dimethylallyl pyrophosphate, (DMAPP) are closely associated with carotenoid biosynthesis. Phenylalanine tyrosine critical substances anthocyanin respectively. Hence, genes such chlI, crtB, PGT8, CYP76AD1, BvDODA can be employed heterologous vitro meet demand increased amount. determinant coloration, an in-depth exploration into high-quality provide basis developing superior offer new insights increasing yield.

Language: Английский

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