The role of Trichoderma koningii and Trichoderma harzianum in mitigating the combined stresses motivated by Sclerotiniasclerotiorum and salinity in common bean (Phaseolusvulgaris)

Plant Stress, Journal Year: 2024, Volume and Issue: 11, P. 100370 - 100370

Published: Jan. 23, 2024

Under natural conditions, crops typically suffer from severe challenges due to the increasing of abiotic and biotic stresses which severely affect plant growth reduc crop yield. The present study investigated single combined impacts Sclerotinia sclerotiorum salinity stress on common bean (Phaseolus vulgaris L.) seedling is scarcely studied. evaluated in vitro vivo influence two tolerant Trichoderma isolates, T. koningii harzianum against S. under stress. results showed ability grow sporulate at high levels salinity, 80 mM NaCl, without significantly impacting their produce cell wall degrading enzymes, cellulase chitinase. Amylase proteinase (Prb1) genes were detected harzianum. assay revealed that both isolates could inhibit concentrations. In a greenhouse experiment, ameliorated damaging seedlings' germination characteristics compared untreated control. Both bioagents attenuated damping-off collar/stem rot percentages infected Salinity intensified effect photosynthetic pigments, induced oxidative nitrative stress, hampered ionic homeostasis, deactivated antioxidants defense-related molecules. On other hand, restrained reduction chlorophylls carotenoids, ascorbate, reduced glutathione, flavonoids, phenolics, various antioxidant especially for All these upregulations reflected keeping membranes beans more stable where lipid peroxidation methylglyoxal reactive oxygen species upregulation nitric oxide, expressed better pathogen attack or/and saline. tested be used as effective biological control saline soils or areas irrigated with water.

Language: Английский

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Harnessing bacterial endophytes for environmental resilience and agricultural sustainability

Journal of Environmental Management, Journal Year: 2024, Volume and Issue: 368, P. 122201 - 122201

Published: Aug. 14, 2024

Language: Английский

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Enhancing the performance of chilli (Capsicum annuum) through twin role of plant growth promotion and disease suppressionvia Bacillus subtilis-based bioformulation

The Indian Journal of Agricultural Sciences, Journal Year: 2024, Volume and Issue: 94(1), P. 039 - 043

Published: Jan. 12, 2024

Considering the majority of studies confined in vitro evaluation microbial bioagents against diseases, a study was carried out during 2018–21 at Assam Agricultural University, Jorhat, Assam, (in vivo and field studies) to assess bio-efficacy B. subtilis (strain LB22)-based liquid bioformulation bacterial wilt (Ralstonia solanacearum) anthracnose (Colletotrichum gloeosporioides) diseases chilli (Capsicum annuum L.). In efficacy formulation (inclusive treatment involving seed treatment, seedling dip, soil foliar spray) showed significant suppressiveness both coupled with enhanced growth yield attributes. Subsequently, Bacillus comparing standard chemicals also edged over latter by 15.45% terms gains 2-years study. A reduction found disease severity on account induced resistance via upregulated synthesis plant defense-enzymes (Polyphenol oxidase, peroxidase, phenylalanine ammonia-lyase, β-1,3-glucanase) 2.3–11.0 folds their peaks mostly expressed within 24–72 h. These results put forward conceptual framework for delivery mechanism bioagent mode, having potential be effective number other as well.

Language: Английский

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Assessment of avocado grafting practices and propagules quality against minimum certification standards in Tanzania

Cogent Food & Agriculture, Journal Year: 2025, Volume and Issue: 11(1)

Published: Jan. 6, 2025

Avocado (Persea americana Mill.) is a promising cash crop in Tanzania, but reliance on poor quality, noncertified propagules hampers the market potential. The Tanzania Official Seed Certification Institute (TOSCI) has established minimum certification standards for nursery-propagated avocado plants to enhance seedling quality. This study evaluated current grafting practices and propagule quality against TOSCI across three regions: Morogoro, Mbeya, Njombe, involving 90 randomly selected growers. Recent findings indicate that 40%, 98.9%, 7.8%, 63.3%, 96.7%, 84.4% of growers adhered recommended nursery isolation distances, utilized local seeds rootstock, treated before prepared media with mixture soil organic matter, did not sterilize equipment, used healthy scions from orchards younger than seven years, respectively. Based 37.8% 64.4% participants rootstock diameter specified size polythene bags. In contrast, point heights all regions conformed standards. For pathogenic fungal 33.3% potting samples tested positive Phytophthora cinnamomi Fusarium oxysporum, These results underscore need improved education extension services foster adherence standards, ultimately enhancing production Tanzania.

Language: Английский

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Co-inoculation of Trichoderma viride with Azospirillum brasilense could suppress the development of Harpophora maydis-infected maize in Egypt

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15

Published: Feb. 6, 2025

Plant diseases caused by fungal pathogens are responsible for severe damage to strategic crops worldwide. Late wilt disease (LWD) is a vascular that occurs late in maize development. Harpophora maydis, the causative agent of LWD, significant economic losses Egypt. Therefore, aim this study was control LWD using an alternative approach reduce use chemical pesticides. A combination Trichoderma viride, biocontrol agent, and Azospirillum brasilense, bacterial endophytic plant growth promoter, applied vitro planta. T. viride showed high mycoparasitic potential against H. maydis via various antagonistic activities, including production lytic enzymes, secondary metabolites, volatile compounds, siderophores. A. brasilense filtrates were also shown suppress growth, addition their ability produce gibberellic indole acetic acids. change metabolites secreted observed GC/MS presence maydis. field experiment conducted on susceptible resistant hybrids evaluate activity combined with incidence as well promotion under conditions. The data revealed decrease both severity plants treated and/or brasilense. Further, there noticeable increase all yield parameters. An anatomical examination inoculated roots reflective responses biotic stress. Taken together, obtained results provide successful eco-friendly management strategies maize.

Language: Английский

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Exploring the Potential Benefits of Trichoderma Species in Agro-Industrial Crop Production

Published: Jan. 1, 2025

Language: Английский

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A newly isolated Trichoderma Parareesei N4-3 exhibiting a biocontrol potential for banana fusarium wilt by Hyperparasitism

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 24, 2023

Banana Fusarium wilt caused by oxysporum f. sp. cubense tropical race4 (Foc TR4) is one of the most destructive soil-borne fungal diseases and currently threatens banana production around world. Until now, there lack an effective method to control wilt. Therefore, it urgent find eco-friendly strategy against disease. In this study, a strain Trichoderma N4-3 was isolated newly from rhizosphere soil plants. The isolate identified as parareesei through analysis TEF1 RPB2 genes well morphological characterization. vitro antagonistic assay demonstrated that had broad-spectrum antifungal activity ten selected phytopathogenic fungi. Especially, strong Foc TR4. results dual culture indicated could grow rapidly during pre-growth period, occupy growth space, secrete series cell wall-degrading enzymes upon interaction with These contributed mycelial spore destruction pathogenic fungus hyperparasitism. Additionally, sequenced genome proved contained 21 encoding chitinase 26 β-1,3-glucanase. electron microscopy showed theses disrupted mycelial, spore, ultrastructure A pot experiment revealed addition significantly reduced amount TR4 in bananas at 60 days post inoculation. disease index decreased 45.00% fresh weight increased 63.74% comparison control. Hence, will be promising biological agents for management plant diseases.

Language: Английский

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Identification and application of an endophytic fungus Arcopilus aureus from Panax notoginseng against crop fungal disease

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 7, 2024

Endophytic fungi are important microbial resources for developing novel antibacterial and antifungal drugs to prevent control crop diseases. Panax notoginseng has been used as a Chinese medicinal herb long time, it various bioactivities. However, information on endophytic isolated from is rare. In this study, an fungus known SQGX-6, which was later identified the golden hair Arcopilus aureus, notoginseng. SQGX-6 extracted using ethyl acetate, active components of were ultra-performance liquid chromatography-mass spectrometry (UHPLC-MS). The antioxidant activities extract determined evaluated in vitro vivo. its inhibited growth Corn stalk rot (Fusarium graminearum), southern leaf blight (Helminthosporium maydis), Tomato gray mold (Botrytis cinerea) vitro. free radical scavenging rates 2,2-Diphenyl-1-pyridinyl hydrazide (DPPH) activity, 3-Ethylbenzothiazoline-6-Sulfonic Acid Radical (ABTS) activity also downregulated by extract. vivo, mycelial three aforementioned malondialdehyde (MDA) content upregulated peroxidase (POD) phenylalanine ammonia-lyase (PAL) fruits, leading significant reduction damage cherry tomatoes caused Botrytis cinerea. UHPLC-MS performed identify substances, including Alkaloids, Azoles, Benzofurans, Coumarins, Flavonoids, Organic acids, Phenols, plant regulators contained These results suggested that have excellent activities, thus, resource against fungal infections.

Language: Английский

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An overview of symbiotic and pathogenic interactions at the fungi-plant interface under environmental constraints

Frontiers in Fungal Biology, Journal Year: 2024, Volume and Issue: 5

Published: Oct. 25, 2024

The complex and dynamic interactions between fungi plants constitute a critical arena in ecological science. In this comprehensive review paper, we explore the multifaceted relationships at fungi-plant interface, encompassing both mutualistic antagonistic interactions, environmental factors influencing these associations. Mutualistic associations, notably mycorrhizal relationships, play pivotal role enhancing plant health balance. On contrary, fungal diseases pose significant threat to health, agriculture, natural ecosystems, such as rusts, smuts, powdery mildews, downy wilts, which can cause extensive damage lead substantial economic losses. Environmental constraints abiotic biotic are elucidated understand their shaping interface. Temperature, moisture, soil conditions, along with presence of other microbes, herbivores, competing plants, significantly influence outcome interactions. interplay mutualism antagonism is emphasised key determinant ecosystem stability. implications extend overall productivity, conservation efforts. potential applications knowledge bioremediation, biotechnology, biocontrol strategies emphasise importance adapting climate change. However, challenges future directions field include impacts change, emerging pathogens, genomic insights, interface restoration ecology. Hence, paper provides overview influences, conservation, restoration.

Language: Английский

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Effectiveness of Bacillus cereus in controlling potato bacterial wilt caused by Ralstonia solanacearum: greenhouse and field studies with insights into resistance-related enzymes in potatoes

Journal of Plant Diseases and Protection, Journal Year: 2023, Volume and Issue: 131(1), P. 65 - 75

Published: Oct. 5, 2023

Language: Английский

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