Optimizing sweet potato production: insights into the interplay of plant sanitation, virus influence, and cooking techniques for enhanced crop quality and food security

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: March 18, 2024

This study investigates the impact of sweet potato plant sanitation on yield and external internal quality root storage exploring nutritional content affected by various cooking methods (raw, boiled, oven-cooked). The presence viruses, concretely leaf curl virus (SPLCV), in propagation material is shown to significantly reduce modify quality. Notably, research reveals a substantial improvement crop quality, reinforcing efficacy methods, specifically apical meristem culture, preserving overall productivity crops. Furthermore, investigation identifies noteworthy decrease starch content, suggesting dynamic interaction between metabolism response viral diseases. also delves into alteration mineral absorption patterns, shedding light how influences uptake essential minerals roots. While health status plants only slightly magnesium (Mg) manganese (Mn) accumulation, indicating potential resilience balance under virus-infected conditions. Moreover, significant modifications antioxidant levels, emphasizing role enhancing potatoes. Heat-treated roots, subjected such as boiling oven-cooking, exhibit notable differences parameters. These include increased concentrations total soluble solids (SS) heightened levels compounds, particularly phenolic flavonoid compounds. observed increase capacity underscores health-promoting benefits associated with practices. Overall, critical importance production sustainability, contributing food security, supporting local agricultural economies. results emphasize need for further optimize promote their widespread adoption globally, providing valuable insights complex relationships

Language: Английский

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Attenuation of Zucchini mosaic virus disease in cucumber plants by mycorrhizal symbiosis

Plant Cell Reports, Journal Year: 2024, Volume and Issue: 43(2)

Published: Feb. 1, 2024

Abstract Key message Arbuscular mycorrhizal fungi generated systemic acquired resistance in cucumber to Zucchini yellow mosaic virus , indicating their prospective application the soil as a sustainable, environmentally friendly approach inhibit spread of pathogens. The wide plant pathogens affects whole world, causing several diseases and threatening national food security it disrupts quantity quality economically important crops. Recently, acceptable mitigating practices have been required for sustainable agriculture, restricting use chemical fertilizers agricultural areas. Herein, biological control (ZYMV) ( Cucumis sativus L.) plants using arbuscular (AM) was investigated. Compared plants, ZYMV-infected displayed high disease incidence (DI) severity (DS) with various symptoms, including severe mosaic, mottling green blisters leaves. However, AM fungal inoculation exhibited 50% inhibition these symptoms limited DS 26% compared non-colonized ones. detection ZYMV by Enzyme-Linked Immunosorbent Assay technique significant reduction AM-inoculated (5.23-fold) Besides, root colonization (F%) slightly reduced infection. infection decreased all growth parameters pigment fractions increased malondialdehyde (MDA) content, however, were significantly enhanced MDA content colonization. Also, protein, proline antioxidant enzymes (POX CAT) more enhancements due Remarkably, defence pathogenesis-related (PR) genes such PR-a, PR-b, PR-10 quickly expressed response treatment. Our findings demonstrated beneficial function triggering against they caused supported potential an environment-friendly method hindering pathogenic microorganisms sustainably.

Language: Английский

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Plant Immunity against Tobamoviruses

Viruses, Journal Year: 2024, Volume and Issue: 16(4), P. 530 - 530

Published: March 29, 2024

Tobamoviruses are a group of plant viruses that pose significant threat to agricultural crops worldwide. In this review, we focus on immunity against tobamoviruses, including pattern-triggered (PTI), effector-triggered (ETI), the RNA-targeting pathway, phytohormones, reactive oxygen species (ROS), and autophagy. Further, highlight genetic resources for resistance tobamoviruses in breeding discuss future directions protection tobamoviruses.

Language: Английский

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Monitoring of potato viruses in the Altai region and their influence on morphometric and biochemical parameters of plants

Bulletin of NSAU (Novosibirsk State Agrarian University), Journal Year: 2025, Volume and Issue: 4, P. 116 - 123

Published: Jan. 24, 2025

Potatoes are one of the most important food crops, and susceptible to diseases, including viral ones. They can lead significant yield losses (up 30-80 %), in case infection by several viruses, this damage increases. The article presents results evaluation seed potatoes Altai Territory (10 potato samples from Kytmanovsky, Pervomaisky districts Barnaul city) its influence on morphometric biochemical parameters plants. presence viruses (X, Y, M, A, S, leafroll virus tuber spindle viroid) was determined RT-PCR using a ‘SINTOL’ set reagents. Biochemical parameters: chlorophyll b concentrations, malonic dialdehyde, peroxidase activity were spectrophotometrically. maximum frequency occurrence region observed for two PVY, PVS. On varieties Colomba Juwel, prevalence Y reached 100 %, S 90-100 %. Tuber viroid (quarantine object), A absent all tested samples. Evaluation indices revealed 1.4-fold decrease number leaves affected relative virus-free plants (variety Colombo). variety Gala, combination Y+S caused reduction leaf plates an increase 1.9 times. Gala resulted 1.27-fold MDA concentration 1.26-fold activity. Combined (PVY + PVS) enhanced negative effect with mono-infection.

Language: Английский

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Suppressing Tymovirus replication in plants using a variant of ubiquitin

PLoS Pathogens, Journal Year: 2025, Volume and Issue: 21(1), P. e1012899 - e1012899

Published: Jan. 27, 2025

RNA viruses have evolved numerous strategies to overcome host resistance and immunity, including the use of multifunctional proteases that not only cleave viral polyproteins during virus replication but also deubiquitinate cellular proteins suppress ubiquitin (Ub)-mediated antiviral mechanisms. Here, we report an approach attenuate infection Arabidopsis thaliana by Turnip Yellow Mosaic Virus (TYMV) suppressing polyprotein cleavage deubiquitination activities TYMV protease (PRO). Performing selections using a library phage-displayed Ub variants (UbVs) for binding recombinant PRO yielded several UbVs bound with nanomolar affinities blocked its function. The strongest UbV (UbV3) candidate had EC 50 0.3 nM inhibited both DUB activity in vitro . X-ray crystal structures UbV3 alone complex reveal inhibitor exists as dimer binds two copies PRO. Consistent our biochemical structural findings, transgenic expression cytosol A suppressed planta , reduction load being correlated level. Our results demonstrate potential protect plants from tymovirus infection, family contain members significant agricultural concern, well other plant express functionally related deubiquitinating activity.

Language: Английский

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Influence of Nutrient Medium Components on In Vitro Tuberization of Solanum tuberosum L. and Subsequent Minituber Production in Aeroponic and Greenhouse Conditions

Life, Journal Year: 2025, Volume and Issue: 15(2), P. 241 - 241

Published: Feb. 5, 2025

Potatoes, a vital global food crop, have shown remarkable adaptability, significantly contributing to security. Technological advancements now enable their cultivation from soil-based systems liquid synthetic nutrient media, even in artificial closed environments without natural light or fertile soil. This study examined the effects of Benzylaminopurine (BAP) and Kinetin (Kin) at concentrations ranging 0 5 mg/L sucrose 20 120 g/L on vitro tuberization, focusing microtuber size, weight, tuberization rate. Nodal segments virus-free 'Red Scarlet' potato plantlets were used as explants. These explants cultured Murashige Skoog (MS) medium solidified with 0.5% agar. The also compared minituber production efficiency under greenhouse aeroponic conditions. highest rate (90%) was achieved 80 3.0 BAP. After induction, microtubers transferred both conditions for further assessment biochemical composition. findings demonstrate potential aeroponics superior method producing high-quality, pathogen-free minitubers. Aeroponics resulted higher yields systems, offering scalable efficient solution seed production.

Language: Английский

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Multiplex reverse transcription polymerase chain reaction for simultaneous detection and identification of multiple sunflower-infecting viruses

Journal of Plant Diseases and Protection, Journal Year: 2025, Volume and Issue: 132(2)

Published: Feb. 12, 2025

Language: Английский

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First Detection and Molecular Characterization of Peach Latent Mosaic Viroid (PLMVd) in Kazakhstan

Pathogens, Journal Year: 2025, Volume and Issue: 14(3), P. 243 - 243

Published: March 3, 2025

Viroids represent obligate plant pathogens composed exclusively of non-protein coding small single-stranded RNAs that cause high economic losses worldwide. A field survey was carried out to assess the incidence peach latent mosaic viroid (PLMVd) in southeastern Kazakhstan, region country where fruit trees are mainly grown. Of 246 stone trees, 20 (8.13%) were infected with PLMVd. The PLMVd (19.23%; 15/78) significantly higher than apricot (6.76%; 5/74; p = 0.0234). Eight detected viroids cloned and used for full-genome sequencing. nucleotide sequence similarity selected isolates found Kazakhstan 83.9–100%. phylogenetic analysis indicated three clusters Kazakhstani Three groups differed their predicted secondary structure. During survey, genetically characterized first time Kazakhstan. obtained results indicate need develop state control measures PLMVd, including regular monitoring surveys. We identified several SNPs had not been previously described. may be useful optimizing diagnostic approaches detecting preventing spread through propagation material.

Language: Английский

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Harnessing plant growth-promoting bacteria to combat watermelon mosaic virus in squash

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 19, 2025

Abstract Plant diseases significantly threaten global food security, with viral infections, particularly Watermelon Mosaic Virus (WMV), causing substantial losses in economically important crops such as squash. This study aims to investigate the efficacy of beneficial bacteria isolated from various plants promoting growth and mitigating effects WMV Understanding interactions between microbes could provide sustainable solutions for managing infections agriculture. Sixty-two bacterial isolates were obtained rhizosphere basil, mint, thyme, squash plants. Among these, six strains exhibited notable plant growth-promoting activities, including synthesis indole acetic acid, solubilization phosphate zinc, ammonia production, activity 1-aminocyclopropane-1-carboxylate deaminase (ACCD). Morphological observations 16S rRNA gene sequencing identified these Pseudomonas indica , Bacillus paramycoides thuringiensis mycoides Paenibacillus glucanolyticus Niallia circulans . In pot experiments, inoculated demonstrated significant reductions disease severity after being infected WMV. Specifically, foliar applications resulted following severity: B. (87%), (73%), (70%), (65%), (65%). Additionally, treated showed increased height shoot dry weight, indicating enhanced performance relative controls. Statistical analysis revealed that promotions reduction ( p < 0.05). GC–MS a diverse array 73 chemical metabolites, common compounds 9-Octadecenoic acid (Z), benzene derivatives, cyclopentanones. These findings suggest shared metabolic pathways among indicate potential roles ecological interactions, defense mechanisms, antiviral properties. metabolites likely contribute observed enhance resilience. The indicates inoculating specific bacteria, especially through or soil application can decrease promote growth. approach offers an environmentally friendly alternative treatments may reduce reliance on pesticides. research highlights using (PGPB)as control crops. Further field trials are necessary PGPB validate scalability assess their effectiveness under agricultural conditions. Incorporating into practices resilience cropping systems, ultimately fostering agriculture enhancing security.

Language: Английский

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Effect of thermotherapy on meristem culture response of two local cassava (Manihot esculenta Crantz) cultivars from Burkina Faso

Discover Plants., Journal Year: 2025, Volume and Issue: 2(1)

Published: March 21, 2025

Language: Английский

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