Acetate prevents pistil dysfunction in rice under heat stress by inducing methyl jasmonate and quercetin synthesis

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Acetic acid (HAC) is a crucial signal molecule in plant stress responses; however, its role conferring heat tolerance to rice remains unclear. This study aims investigate the effect of HAC protecting pistil function under and potential facilitating pollen germination tube growth via HAC-induced synthesis methyl jasmonate (MeJA) quercetin (QR). Physiological analysis, including germination, into ovule, reactive oxygen species (ROS), as well levels HAC, acetyl coenzyme A (acetyl-CoA), MeJA, QR pistils stress-treated early indica cultivars Zhongzao39 (ZZ39) Zhongjiazao17 (ZJZ17), were conducted. RNA sequencing (RNA-seq) was performed identify differentially expressed genes involved this process. Effect exogenous acetate (NaAC), on spikelet fertility also investigated. Compared with ZJZ17, severe inhibition fertility, observed ZZ39, due ROS burst an irregular distribution across stigma, style, ovule. RNA-seq physiological data indicate that may activate acetyl-CoA enhance by inducing MeJA QR. Exogenous NaAC enhanced stress, accompanied elevated antioxidant enzyme activities, improved energy status, increased acetyl-CoA, pistils. Additionally, NaAC, QR, either alone or combination, effectively augmented while combination inhibitors significantly reduced fertility. Acetate activates induce both thereby alleviating heat-induced dysfunction maintaining homeostasis enhancing Our results offer promising strategy crops.

Language: Английский

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Influence of Exogenous Melatonin on the Physiological Traits of Camellia hainanica Seedlings Under Polyethylene Glycol-Induced Drought Stress

Plants, Journal Year: 2025, Volume and Issue: 14(5), P. 676 - 676

Published: Feb. 22, 2025

This study investigated the effects of exogenous melatonin (MT) on physiological responses Camellia hainanica seedlings under drought stress, using drought-tolerant variety “Hai Da 1” and drought-sensitive “Wan Hai as test materials. Seedlings were treated with MT at concentrations 0, 50, 100, 150, 200, 250 μmol/L through irrigation, followed by stress induced polyethylene glycol (PEG-6000). The results revealed that alleviated growth damage caused PEG-simulated leaf relative conductivity malondialdehyde (MDA) content showing an initial decrease increase concentration rose. In contrast, water content, chlorophyll antioxidant enzyme activity, secondary metabolite levels, carbohydrate initially increased then declined increasing concentration. Treatment 200 notably reduced MDA 40–50%, enhanced activity 20–30%, levels 11–42% in variety. These findings identified optimal for mitigating C. seedlings, providing a foundation its practical application production further research into resistance mechanisms mediated MT.

Language: Английский

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Identification of the maize drought-resistant gene Zinc-finger Inflorescence Meristem 23 through high-resolution temporal transcriptome analysis

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142347 - 142347

Published: March 1, 2025

Language: Английский

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An A-6 subgroup member of DREB gene family positively regulates cold stress tolerance by modulating an antioxidant defense system in transgenic potato

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: May 2, 2025

Cold stress adversely influences the growth, development, geographic distribution, and yield of plants. The dehydration-responsive element binding (DREB) transcription factors are central to improving plant's ability endure cold stress. In this work, expression pattern StDREB30 (A-6) gene was analyzed in response transgenic potato. We provide evidence emphasizing significance under low-temperature (4°C) investigate potential physiological, molecular biochemical processes involved. levels were quickly elevated upon exposure. Additionally, potato plants exhibited upregulation randomly selected downstream genes (StNAC, StDREB1, StDREB2, StSAP, StGT3, StDHN), improved photosynthetic parameters including coefficient photochemical quenching (qL), maximum PSII (Fv'/Fm'), better stomatal performance, increased proline accumulation, decreased malondialdehyde content, electrolyte leakage, reduced accumulation hydrogen peroxide, superoxide when exposed Moreover, reactive oxygen scavenging capabilities by stimulating production antioxidants such as dismutase, peroxidase, catalase, ascorbate peroxidase. Our results propose that serves a positive regulator promoting tolerance. To our knowledge, no report has been published previously on study potatoes.

Language: Английский

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Enhanced Antioxidant Activity and Secondary Metabolite Production in Tartary Buckwheat under Polyethylene Glycol (PEG)-Induced Drought Stress during Germination

Agronomy, Journal Year: 2024, Volume and Issue: 14(3), P. 619 - 619

Published: March 19, 2024

Drought stress is one of the key factors impeding agricultural productivity worldwide. This experiment aimed at investigating polyethylene glycol (PEG)-induced drought effects on seed germination, physiology, and biochemical mechanisms in Tartary buckwheat genotypes. Four PEG-induced conditions (0%, 10%, 20%, 30%) were applied to 14 selected genotypes germination stage evaluate their tolerance capacity. Significant differences obtained percentage, relative water content (RWC), all growth parameters among studied Based index (STI), XiNong 9943, 9940, QianKu-5 found be tolerant, QuanKu-4 was susceptible. These cultivars for further physiological characterization. The results demonstrated that activity enzymes significantly increased with increase PEG dose. SOD (superoxide dismutase), POD (peroxidase), CAT (catalase), APX (ascorbate peroxidase) levels 30% 9943 genotype 2.01, 2.19, 4.92, 4.46 times higher, respectively, than normal condition (T0). Moreover, secondary metabolite also At PEG, yielded phenols, flavonoids, polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL) higher by 131%, 95%, 154%, 164%, T0 condition. From both findings content, genotypic response ranked following order: > 9940 QianKu-4, which supported STI selection system. Assessing overall performance, shows tolerance, can useful material future breeding programs.

Language: Английский

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Sexually dimorphic acetyl‐CoA biosynthesis and utilization in response to drought and exogenous acetic acid

The Plant Journal, Journal Year: 2024, Volume and Issue: 119(4), P. 1967 - 1985

Published: June 30, 2024

SUMMARY Female willows exhibit greater drought tolerance and benefit more from exogenous acetic acid (AA)‐improved than males. However, the potential mechanisms driving these sex‐specific responses remain unclear. To comprehensively investigate sexually dimorphic responsive of to AA, here, we performed physiological, proteomic, Lys‐acetylproteomic, transgenic analyses in female male Salix myrtillacea exposed AA‐applicated treatments, focusing on protein abundance lysine acetylation (LysAc) changes. Drought‐tolerant females suffered less drought‐induced photosynthetic oxidative damage, did not activate AA acetyl‐CoA biosynthesis, TCA cycle, fatty metabolism, jasmonic signaling as strongly drought‐sensitive Exogenous caused overaccumulation endogenous inhibition biosynthesis utilization greatly enhanced further performance females, possibly determining that improved Interestingly, overexpression synthetase ( ACS ) could reprogram acids, increase LysAc levels, improve tolerance, highlighting involvement ACS‐derived responses. In addition, induced associated with histones, transcription factors, metabolic enzymes willows. Especially, may capacity S. males by decreasing levels increasing abundances proteins. While hyperacetylation glycolysis, potentially serve negative feedback acclimate drought‐stressed females. Thus, determine AA.

Language: Английский

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Harnessing Bacillus safensis as Biofertilizer for Sustainable Drought Alleviation in Brassica juncea L.

Biocatalysis and Agricultural Biotechnology, Journal Year: 2024, Volume and Issue: unknown, P. 103388 - 103388

Published: Sept. 1, 2024

Language: Английский

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Biochemical, physiological, and molecular responses of diverse olive cultivars to different irrigation regimes

Notulae Botanicae Horti Agrobotanici Cluj-Napoca, Journal Year: 2023, Volume and Issue: 51(4), P. 13395 - 13395

Published: Dec. 18, 2023

Olive is an essential industrial crop in the Mediterranean region with valuable economic and agricultural concerns. Despite its drought resistance, productivity restricted by extreme stress. cultivars display considerable variation response mechanisms to Accordingly, impact of mild water deprivation over two seasons compared full irrigation requirements on growth physiological characteristics three diverse olive cultivars. Three cultivars, ‘Manzanillo’, ‘Eggizi-Shami’, ‘Tofaahi’, were evaluated under regimes 100% ETc, 75% 50% ETc. Characteristics shoot root, as well physio-chemical constituents, determined. Besides, gene expression dehydration-responsive element binding (DREB), dehydrin (DHN), catalase (CAT) genes explored different regimes. The results indicated a substantial level all studied parameters. stress treatments caused gradual reduction nitrogen, phosphorus, potassium content, relative root length, leaf numbers, branch count, area across both seasons. Conversely, proline content was considerably increased well-watered conditions. Similarly, assessed exhibited significant parameters, ‘Eggizi-Shami’ demonstrating superiority. Under conditions, cultivar displayed highest most characteristics. real-time quantitative PCR (RT-qPCR) analysis alterations tested related (DHN, DREB, CAT). RT-qPCR revealed that conditions (75% ETc), higher other (‘Tofaahi’ ‘Manzanillo’). Combining morphological parameters drought-related can offer highly validated information about drought-tolerant This integrated approach serves innovative methodology identify confirm involved abiotic

Language: Английский

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Exploring the Impact of High-Temperature Stress on Physiological, Anatomical, Molecular, Productive, and Quality Parameters in Diverse Olive Cultivars

Deleted Journal, Journal Year: 2024, Volume and Issue: 106(4), P. 445 - 458

Published: April 8, 2024

Olive (Olea europaea L.) is one of the most important horticultural crops in Saudi Arabia. High temperatures are typical regions during summer season. This study explored effect high on diverse olive cultivars (Manzanillo, Chemlali, and Picual) grown three different geographical locations Arabia (Sakaka, Basita, Al-Qurayyat). Sakaka region was classified as a hot region, while Basita Al-Qurayyat were characterized by relatively mild or low temperatures. Genotypic components assessed studying Amplified Fragment Length Polymorphism (AFLP) molecular markers physiological, anatomical, productive, oil quality parameters. The evaluated genotypes displayed considerable variation across studied locations. Picual exhibited more heat tolerance, followed Chemlali Manzanillo. temperature negatively impacted critical characteristics related to growth, production, quality. Results revealed that linoleic acid, acidity, total phenols, upper epidermis, palisade tissue, vascular bundle area, mesophyll appropriate parameters for predicting impact These traits highly correlated with significantly associated all markers. Also, significant interactions between showed means highest region.

Language: Английский

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Exploring Plant Responses to External Stimuli (Acetic Acid and Salt) Through Machine Learning Models: Integrating Image and Sensor Data Analysis

Published: Jan. 1, 2024

Language: Английский

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