The process of nitrogen-adaptation root endophytic bacterial rather than phosphorus-adaptation fungal subcommunities construction unveiled the tomato yield improvement under long-term fertilization

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 17, 2025

Interactions between endophytes (endophytic bacteria and fungi) plants are crucial in maintaining crop fitness agricultural systems, particularly relation to abundant rare subcommunities involved community construction. However, the influence of long-term fertilization on heterogeneous rhizosphere nitrogen phosphorus environments how these conditions affect key root their assembly mechanisms remain unclear. We studied 26th year a field experiment conducted greenhouse with varying levels (CKP0, CKP1, CNP0, CNP1, ONP0, ONP1) assess composition tomato impact yield. employed 16S rRNA fungal ITS region amplicon sequencing investigate endophytic subcommunities, network correlations, core subcommunity structures, species that enhance The results indicated organic manure fertilizers significantly increased soil content, availability (labile P, moderately labile non-labile P). These also affected (based Bray-Curtis distance) processes (βNTI) microbial subcommunities. both bacterial was primarily governed by dispersal limitation, structures being regulated content available (AN) P (MLP). Rare complemented ecological niches co-occurrence network, supporting functions enhancing stability. Nitrogen-adapting provided stronger predictive correlation for yield than phosphorus-adapting Additionally, three genera such as Arthrobacter, Microbacterium, Sphingobium were identified potentially improving improvement. findings revealed distinct fertilization, our understanding better management practices controlling improve intensive ecosystems.

Language: Английский

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Postlarval Shrimp-Associated Microbiota and Underlying Ecological Processes over AHPND Progression

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 720 - 720

Published: March 24, 2025

Postlarval shrimp frequently face threats from acute hepatopancreatic necrosis disease (AHPND). Although AHPND affects both postlarval and adult shrimp, abiotic biotic factors are distinct between life stages, such as rearing water nutrient levels host stage-dependent microbiota. The response of postlarvae-associated microbiota to AHPND, however, remains largely unexplored compared with its effects on juvenile shrimp. To address this knowledge gap, a comparative analysis the ecological processes underlying progression was performed by sequencing bacterial V3–V4 hypervariable region 16S rRNA gene. infection validated high copies pirAB genes (Toxin 1) in diseased hepatopancreas. Advanced significantly altered structure microbiota, significant enrichment Bacilli Bdellovibrionia species healthy larvae matched AHPND-infected cohorts, although gut recovery observed at late stage, corresponding cessation mortality. explained 11.0% (p < 0.001) variance community structures, whereas postlarvae days post hatching also influenced communities (7.1% variance, p 0.001). exhibited reduced homogeneous selection increased dispersal limitation drift governing their These changes were primarily driven specific microbial lineages, including enriched Bin36 Rhodobacteraceae Bin11 Flavobacteriaceae, suppressed Bin63 Vibrio Bin9 Bacillus After excluding age effect, 13 AHPND-discriminatory taxa identified, accurately distinguishing infected individuals 100% precision. Furthermore, outbreak weakened network complexity stability, which keystone that positively associated robustness. Collectively, our findings deepen understanding inextricable interplay health, dynamics, survival, well mechanisms over progression.

Language: Английский

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Random mechanisms govern bacterial succession in bioinoculated beet plants

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 28, 2025

Plant colonization by microbes is an example of succession, with its distinct phases differing in community structure and diversity. This process needs to be studied improve bioinoculation strategies. Here, we show that, regardless bioinoculation, soil type plant genotype, bacteria colonize the rhizosphere tissues axenic beets two associated taproot development. Communities remained stable after five weeks growth soil. Time, genotype determined both endosphere. Inoculation changed structure, members Pseudomonadota Bacillota were recruited beets. Axenic beet runs through similar a glacier forefront, are mostly randomly. The transition from early late phase involves decrease bacterial load tissues, which may linked arrest cell division. Therefore, inoculation seems favourable. Five enabled formation communities influence indirect, probably due microbe-microbe interactions.

Language: Английский

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Taxonomic and functional β-diversity patterns reveal stochastic assembly rules in microbial communities of seagrass beds

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 28, 2024

Microorganisms are important members of seagrass bed ecosystems and play a crucial role in maintaining the health seagrasses ecological functions ecosystem. In this study, we systematically quantified assembly processes microbial communities fragmented beds examined their correlation with environmental factors. Concurrently, explored relative contributions species replacement richness differences to taxonomic functional β-diversity communities, investigated potential interrelation between these components, assessed explanatory power The results suggest that stochastic dominate community assembly. Taxonomic governed by replacement, while for β-diversity, contribution slightly outweighs processes. A weak but significant ( p &lt; 0.05) exists two components taxonomy functionality, almost no observed This implies taxonomy, convergence redundancy within communities. Environmental factors insufficient explain differences. conclusion, is patterns provide new insights evidence better understanding rules. has implications conservation management beds.

Language: Английский

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Are plant traits drivers of endophytic communities in seasonally flooded tropical forests?

American Journal of Botany, Journal Year: 2024, Volume and Issue: unknown

Published: July 16, 2024

Abstract Premise In the Amazon basin, seasonally flooded (SF) forests offer varying water constraints, providing an excellent way to investigate role of habitat selection on microbial communities within plants. However, variations in community among host plants cannot solely be attributed environmental factors, and how plant traits contribute assemblages remains open question. Methods We described leaf‐ root‐associated using ITS2 16 S high‐throughput sequencing investigated stochastic‐deterministic balance shaping these assemblies two null models. Plant ecophysiological functioning was evaluated by focusing 10 leaf root 72 seedlings, belonging seven tropical SF tree species French Guiana. then analyzed drove assembly endophytic communities. Results While both stochastic deterministic processes governed endophyte leaves roots, stochasticity prevailed. Discrepancies were found between fungi bacteria, highlighting that microorganisms have distinct ecological strategies Traits, especially traits, spatial predictors better explained diversity than composition, but they modest overall. Conclusions This study widens our knowledge about forests, a sensitive climate change, through combined analyses their associated with functional traits. emphasize need other disentangle drivers relationship seedlings microbiomes, ultimately enhancing adaptive capacities change.

Language: Английский

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Roots of Resistance: Unraveling Microbiome-Driven Plant Immunity

Plant Stress, Journal Year: 2024, Volume and Issue: 14, P. 100661 - 100661

Published: Nov. 6, 2024

Language: Английский

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Colonization of beet by rhizosphere bacteria takes place in discreet phases regardless of bioinculation with wild sea beet root community

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: March 4, 2024

Abstract Background: Bioinoculation can increase crop yields under environmental stress. Inoculants consisting of microbial communities instead single strains may be broader use than the latter ones, as different plants might recruit specific organisms from a large pool. Inoculation influences rhizosphere and endophytic community structure modifying assembly process, which is driven by selection due to host microbe-microbe interactions. Plant colonization microbes an instance succession, with its distinct phases differing in diversity. This process needs studied determine optimal timing for bioinoculation studying effects. We wanted learn if, if so, when stable bacterial form during axenic beet bacteria soil, either inoculated wild or non-inoculated this depends on genotype soil. Results: Here we show that regardless bioinoculation, soil type plant colonize beets tissues two load, degree nestedness, structure, diversity well mechanisms aligned taproot development. Both endosphere remain after five weeks growth Alpha-diversity was higher load lower late samples early ones. Communities were more similar at level predicted functional potential amplicon sequence variants (ASVs). Time, determined but not alpha-diversity, nestedness both endosphere. slightly significantly changed roots leaves. Pseudomonadota (formerly Proteobacteria) Bacillota Firmicutes) low abundance coming inoculant found recruited beets. Conclusions: Axenic runs through those other instances succession are mostly randomly. Transition phase involves drop tissues, linked cells division arrest. Therefore, inoculation seems right choice. Five enable formation influence indirect, probably

Language: Английский

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Interplay between Vanadium Distribution and Microbial Community in Soil-Plant System

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 480, P. 136303 - 136303

Published: Oct. 30, 2024

Language: Английский

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Rhizosphere bacterial colonization of beet occurs in discrete phases regardless of bioinoculation with the wild sea beet root community

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Feb. 10, 2023

Abstract Bioinoculation can increase crop yields under environmental stress. Plant colonization by microbes is an example of succession, with its distinct phases differing in community structure and diversity. This process needs to be studied determine the optimal timing for bioinoculation effects. Haere, we show that, regardless bio-inoculation, soil type plant genotype, bacteria colonize rhizosphere axenic beets tissues two phases, bacterial load, nestedness, structure, diversity assembly mechanism, associated taproot development. Communities remained stable after five weeks growth soil. The alpha was greater load lower late samples than early ones. Time, genotype determined but not diversity, nestedness or mechanisms both endosphere. Inoculation changed members Pseudomonadota Bacillota low abundance inoculant were recruited beets. Axenic beet occurs through similar other instances microbial are mostly randomly. transition from phase involves a decrease tissues, which may linked arrest cell division. Therefore, inoculation seems favourable. Five enabled formation communities influence indirect, probably due microbe-microbe interactions.

Language: Английский

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