Cotton Verticillium wilt monitoring based on UAV multispectral-visible multi-source feature fusion

Computers and Electronics in Agriculture, Journal Year: 2024, Volume and Issue: 217, P. 108628 - 108628

Published: Jan. 21, 2024

Language: Английский

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In the coevolution of cotton and pathogenic fungi, resistant cotton varieties lead to an escalation in the virulence of Verticillium dahliae

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 290, P. 117730 - 117730

Published: Jan. 1, 2025

Verticillium dahliae is highly prone to pathogenic differentiation and influenced by host cotton's resistance. To better understand the mechanisms of this phenomenon, we applied selective pressures resistant susceptible cotton varieties on V. strain Vd076 within an artificial wilt nursery greenhouse. Consequently, among offspring strains, high virulence strains exhibited higher levels physiological characteristics genetic diversity compared moderate low strains. Moreover, whole genome resequencing revealed that Ka/Ks ratio single nucleotide polymorphism (SNPs) in majority was about 0.6, indicating adverse selection impact Pathogenicity assays demonstrated triggered continuous induction disease-resistant cultivar increased from 4th generation reached its peak 6th generation. Additionally, transcriptome analysis generations differentially expressed genes (DEGs) accumulated a significant number response associated with pathogen pathogenicity differentiation, including mitogen-activated protein kinase (MAPK) signaling pathway, amino antibiotic biosynthesis, phenylpropanoid metabolism. Furthermore, VDAG_04757, VDAG_06462, VDAG_03218, VDAG_08487 correlation dahliae. Collectively, study has implications for elucidating evolution induced host, as well advancing holistic strategies preventing managing disease.

Language: Английский

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Identification and Application of the Heptad Repeat Domain in the CPR5 Protein for Enhancing Plant Immunity

Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: 26(2)

Published: Feb. 1, 2025

ABSTRACT Plant resistance to pathogens can be significantly enhanced through genetic modification, thereby reducing the reliance on chemical pesticides. CONSTITUTIVE EXPRESSER OF PATHOGENESIS‐RELATED GENES 5 (CPR5) serves as a key negative regulator of plant immunity. Here we explored functional domains CPR5 protein with goal dampening its activity bolster Using hexapeptide asparagine–alanine–alanine–isoleucine–arginine–serine (NAAIRS) linker‐scanning analysis, identified heptad repeat domain (HRD) in middle region protein, which is highly conserved across kingdom. The HRD predicted form an α‐helix structure and acts interface for dimerization. Intriguingly, overexpression Arabidopsis wild‐type plants resulted phenotype similar cpr5 mutant led enhancement immunity, indicating that introduced HRDs disrupt native dimers, relieving suppression Furthermore, expression under control pathogen‐inducible promoter improved cotton Verticillium dahliae , destructive wilt pathogen affecting production worldwide. These findings suggest downregulating by could promising approach strengthening

Language: Английский

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Detection of Cotton Verticillium Wilt Disease Severity Based on Hyperspectrum and GWO-SVM

Remote Sensing, Journal Year: 2023, Volume and Issue: 15(13), P. 3373 - 3373

Published: July 1, 2023

In order to address the challenge of early detection cotton verticillium wilt disease, naturally infected plants in field, which were divided into five categories based on degree disease severity, have been investigated this study. Canopies analyzed with spectral data measured, and various preprocessing techniques, including multiplicative scatter correction (MSC) MSC-continuous wavelet analysis algorithms, used predict severity. With a combination support vector machine (SVM) models such optimization algorithms as genetic algorithm (GA), grid search (GS), particle swarm (PSO), grey wolf optimizer (GWO), grading model was established The study results show that MSC-PSO-SVM outperforms other three terms classification accuracy, macro precision, recall, F1-score are 80%, 81.26%, 79.57%, respectively. Among those eight constructed basis continuous analyses using mexh db3, MSC-db3(23)-PSO-SVM MSC-db3(23)-GWO-SVM perform best, latter having shorter running time. An overall evaluation shows is an optimal model, values its indicators being 91.2%, 92.02%, 91.16%, Moreover, under prediction accuracy levels 1 5 has achieved highest rate 100%, 88% level 2 lowest 84% both 3 4. These demonstrate it effective use technology classifying satisfying needs field grading. This provides new approach for offered theoretical prevention, precise drug application, instrument development disease.

Language: Английский

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Genome-Wide and Expression Pattern Analysis of the DVL Gene Family Reveals GhM_A05G1032 Is Involved in Fuzz Development in G. hirsutum

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(2), P. 1346 - 1346

Published: Jan. 22, 2024

DVL is one of the small polypeptides which plays an important role in regulating plant growth and development, tissue differentiation, organ formation process coping with stress conditions. So far, there has been no comprehensive analysis expression profile function cotton gene. According to previous studies, a candidate gene related development fuzz was screened, belonging family, trichomes Arabidopsis thaliana. However, identification systematic have not conducted. In this study, we employed bioinformatics approaches conduct novel structural characteristics, phylogenetic tree, structure, pattern, evolutionary relationship, selective pressure family members four species. A total 117 genes were identified, including 39 G. hirsutum. Based on analysis, protein sequences categorized into five distinct subfamilies. Additionally, successfully mapped these onto chromosomes visually represented their structure information. Furthermore, predicted presence cis-acting elements hirsutum characterized repeat types Moreover, computed Ka/Ks ratio homologous across species elucidated acting genes. addition, described patterns using RNA-seq data, verified correlation between GhMDVL3 through VIGS technology, found that some may be involved resistance biotic abiotic conditions qRT-PCR technology. potential interaction network constructed by WGCNA, our findings demonstrated GhM_A05G1032 interact numerous genes, thereby playing crucial development. This research significantly contributed comprehension upland cotton, establishing solid basis for future investigations functional aspects cotton.

Language: Английский

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Insights into the biocontrol and plant growth promotion functions of Bacillus altitudinis strain KRS010 against Verticillium dahliae

BMC Biology, Journal Year: 2024, Volume and Issue: 22(1)

Published: May 20, 2024

Abstract Background Verticillium wilt, caused by the fungus dahliae , is a soil-borne vascular fungal disease, which has great losses to cotton yield and quality worldwide. The strain KRS010 was isolated from seed of wilt-resistant Gossypium hirsutum cultivar “Zhongzhimian No. 2.” Results broad-spectrum antifungal activity various pathogenic fungi as Botrytis cinerea Fusarium spp., Colletotrichum Magnaporthe oryzae inhibition rate V. mycelial growth 73.97% 84.39% respectively through confrontation test volatile organic compounds (VOCs) treatments. identified Bacillus altitudinis phylogenetic analysis based on complete genome sequences, physio-biochemical characteristics were detected, including growth-promoting ability active enzymes. Moreover, control efficiency against wilt 93.59%. After treatment with culture, biomass reduced. in group (Vd991 alone) 30.76-folds higher than that (KRS010+Vd991). From molecular biological aspect, could trigger plant immunity inducing systemic resistance (ISR) activated salicylic acid (SA) jasmonic (JA) signaling pathways. Its extracellular metabolites VOCs inhibited melanin biosynthesis . In addition, had been characterized promote growth. Conclusions This study indicated B. beneficial microbe potential for controlling cotton, well promoting

Language: Английский

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Optimization of the fermentation media and growth conditions of Bacillus velezensis BHZ-29 using a Plackett–Burman design experiment combined with response surface methodology

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: April 22, 2024

Introduction Bacillus velezensis occurs extensively in the soil environment. It produces a range of antimicrobial compounds that play an important role field biological control. However, during actual application process it is often affected by factors such as medium formulation and fermentation conditions, therefore biocontrol measures do not achieve their expected outcomes. Methods In this study, B. BHZ-29 strain was used research object. The carbon nitrogen sources, inorganic salts affect number viable bacteria antibacterial potency BHZ-29, were screened single factor test. A Plackett–Burman design experiment conducted to determine significant affecting potency, Box–Behnken obtain optimal growth BHZ-29. formula produced highest most substances determined. initial pH, temperature, amount inoculant, liquid volume, shaking speed, culture time determined had influence on selected orthogonal titer. Results Molasses, peptone, magnesium sulfate effects count titer increased from 7.83 × 10 9 2.17 CFU/mL, 111.67 153.13 mm/mL when media used. conditions for follows: temperature 25.57°C, pH 7.23, 95.90 h, rotation speed 160 rpm, inoculant 2%, volume 100 ml. After optimization 3.39 bacteriostatic 158.85 mm/ml. plant height leaf cotton plants treated with broth higher than those inoculated Verticillium dahliae . 1.15 7 CFU/g, fungi 1.60 5 spores/g. disease index seedlings optimized 2.2, control effect 93.8% achieved. could reduce wilt controlling disease. best achieved treatment group inoculation concentration 2 8 CFU/ml, 14.50, 84.18% Discussion parameters lay foundation practical production agriculture.

Language: Английский

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Role of Verticillium dahliae effectors in interaction with cotton plants

Phytopathology Research, Journal Year: 2025, Volume and Issue: 7(1)

Published: Jan. 2, 2025

Abstract Cotton ( Gossypium spp.) is grown worldwide owing to the vast economic value of its natural fiber. However, widespread and destructive soilborne pathogen, Verticillium dahliae , causes wilt, leading severe yield losses reduced fiber quality cotton. The ability V. perceive infect cotton determines outcome their interactions. employs diverse defense mechanisms evade or suppress plant immunity, ultimately establishing a proliferation niche. Evading immunity by suppressing host recognition successive immune signaling successful infection strategy employed various microbial pathogens, posing significant challenge effectively utilizing hereditary resistance genes in sustainable disease management. This review focused on summarizing “effectors” molecular effectors corresponding plants. Furthermore, it highlighted potential for engineering plants against aiming provide reference creation disease-resistant germplasm resources genome editing other methods.

Language: Английский

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Phenotypic Reaction of Cultivated and Wild Brinjal Genotypes to Fusarium Wilt Under Sick Plot and Artificially Inoculated Pot Conditions

Journal of Phytopathology, Journal Year: 2025, Volume and Issue: 173(1)

Published: Jan. 1, 2025

ABSTRACT Brinjal ( Solanum melongena L.), an important solanaceous vegetable valued for its rich nutritional profile and potential health benefits, is widely cultivated in the tropical subtropical regions of world. Existing food security concerns are exacerbated due to rise soil borne pathogens, a direct consequence global warming, increasing risk crop diseases. Among these, Fusarium wilt, caused by multiple species , very serious severity persistent nature. Traditional control measures, including cultural, biological chemical approaches, have proven inadequate, highlighting need resistant cultivars. The current study focused on identifying fungal pathogen associated with brinjal employing morpho‐cultural identification, molecular characterisation sequencing DNA barcoding internal transcribed spacer (ITS) β‐tubulin pathogenicity assays isolate. oxysporum f. sp. melongenae was identified as vascular wilt brinjal. evaluated 90 diverse genotypes, released varieties local landraces brinjal, related wild resistance against screening under field conditions. Confirmatory evaluation disease reaction done artificially inoculated conditions pots. Percent incidence (PDI), area progress curve (AUDPC) stairs (AUDPS) were used identify sources understand progression patterns. Three genotypes namely Swarna Mani, S. sisymbriifolium torvum highly seven G‐17, Pink, CH‐151, Sidhasar Local, Special Muktakeshi, H‐183 Pink Shiny Medium Long found both pot Resistant exhibited distinct variations hold significant breeding programs aimed at enhancing resilience threat posed species.

Language: Английский

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Thidiazuron as a defoliant to facilitate mechanical harvesting in cotton: A comprehensive review

Plant Science Today, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 12, 2025

Cotton is primarily cultivated for its commercial fiber, which plays a significant role in India’s agro-industrial sector. It one of the primary raw materials producing feed, oil, and biofuel. Currently, farmers India widely employ machine harvesters to harvest cotton. However, excessive leaf vegetation poses challenges boll picking, adversely affecting fiber quality reducing mechanical harvesting efficiency. Various chemical defoliants are applied remove leaves before address this issue. These promote shedding, minimize debris cotton, enhance opening picking Thidiazuron potent hormonal defoliant used cotton induce defoliation by increasing ethylene production while inhibiting synthesis transport auxins. Notably, it interferes with crosstalk between phytohormones, such as cytokinin ethylene, regulates defoliation. The method timing application crucial improving This review aims provide clear understanding thidiazuron’s synchronizing harvests, ultimately supporting mechanization harvesting.

Language: Английский

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