Optimization of culture conditions for the production, antifungal activity and characterization of secondary metabolites of <i>Trichoderma longibrachiatum</i> DOI Open Access

R. Priya,

S. Balachander,

N. Prabhakaran

et al.

Journal of Biological Control, Journal Year: 2023, Volume and Issue: unknown, P. 131 - 144

Published: Sept. 30, 2023

The present study was intended to optimize the culture conditions for secondary metabolite production by endophytic fungi. Based on morphology and phylogeny, fungus identified as Trichoderma longibrachiatum isolated from brinjal leaf based morphological characterization. antifungal activity evaluated against phytopathogens such Macrophomina phaseolina, Phytopthora infestans, Colletotrichum falcatum gloeosporioides through overlapping method, filtrate organic fraction Potato dextrose both a growth medium. exhibited significant activity, while modifications in medium composition may possess major impact quantity quality of metabolites production. To achieve maximum production, optimized with screening basal media, carbon, nitrogen, pH, trace elements incubation period. final fermentation were Minimal ereavis broth media; glucose sucrose carbon source; Peptone Yeast extract nitrogen source, sodium nitrate precursor; pH 6; period 7 days at 28°C. This optimization resulted 47.19-60.67% M. P. C. falcatum, which higher than that before (43.80%). GCMS revealed distinct T. longibrachiatum, comprising molecules additional bioactivities. These results strengthen ongoing research disease control agriculture emphasizing biocontrol potential phyllosphere plant pathogenic

Language: Английский

Systemic defense induced by fatty acid compounds from marine macroalgae, Chaetomorpha antennina in tomato (Solanum lycopersicum) plants alters the susceptibility of the polyphagous agricultural pest, Spodoptera litura Fab DOI Creative Commons

Chanthini Kanagaraj Muthu-Pandian,

Pandian Kirupaanntha Rajan,

Arulsoosairaj Deva-Andrews

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 29, 2023

Abstract Background Seaweeds contain a widespread range of fatty acids (FA), and several them have potential bioactivity. FAs are dynamic members all biota, as well being acknowledged for their critical function in initiating phytohormone interactions acting important participants many defence signalling pathways the plant system. The current study looks at defense-eliciting potentials from green seaweed Chaetomorpha antennina impact on polyphagous insect pest Spodoptera litura (Fab). Results was detected with 19 acids, larger proportion hexa octadecanoic linoleic acids. algal acid compounds (CFA) successful eliciting salicylic phenolic biosynthesis along enzymes peroxidase (PO) polyphenol oxidase (PPO). defense phenol levels increased post infestation S. . CFA also effective causing direct mortalities to larvae (II-V instars). exposed elicited tomato plants were severely affected physiologically morphologically, displaying visible aberrations morphogenetic defects such altered larval-pupal duration biomass. Reproductive performances adults affected. Decrease food utilization, nutritional indices corresponding decrease phosphatase gut affirm feeding deterrence larvae, which endorsed by histological analysis midgut cell disruption larvae. Detoxification enzyme denote inability larval immune system evade harmfulness CFA. Conclusion Hence, finally confirms elicitor C. , inducing natural systemic defences. This investigation unlocks novel forecasts besides delivering an unconventional method crop protection moderate or interchange solicitation chemical pesticides.

Language: Английский

Citations

0

Characteristics of Aluminum Accumulation and Distribution in Leaves of Different Ages in Camellia oleifera DOI Open Access

Jia Lu,

Xinjing Qu, Jian Li

et al.

Forests, Journal Year: 2023, Volume and Issue: 14(12), P. 2322 - 2322

Published: Nov. 27, 2023

Oil tea (Camellia oleifera Abel.), an important edible oil tree found in the acidic soil of south China, is tolerant to aluminum (Al); however, mechanism via which it detoxifies Al remains unclear. The present study investigated accumulation and distribution changes antioxidant components C. leaves at different stages growth. analysis revealed that content young, mature, old was 1.53, 7.49, 12.04 g kg−1, respectively. In leaves, mainly accumulated cell wall, accounted for 66.03%, 79.94%, 86.51% total leaf hemicellulose increased from 54.22% 81.72% with age. proportion upper epidermis, lower palisade, spongy parenchyma walls activity enzymes showed a synergistic effect on stress peroxidase highest mature 2.3 times young leaves. Meanwhile, catalase superoxide dismutase activities phenol were Correlation system composed catalase, (CAT, SOD, TP) negatively correlated malondialdehyde (MDA) positively indicating MDA helped resist oleifera. Thus, our findings suggest increase properties modification wall contributed detoxification hyperaccumulator

Language: Английский

Citations

0

Optimization of culture conditions for the production, antifungal activity and characterization of secondary metabolites of <i>Trichoderma longibrachiatum</i> DOI Open Access

R. Priya,

S. Balachander,

N. Prabhakaran

et al.

Journal of Biological Control, Journal Year: 2023, Volume and Issue: unknown, P. 131 - 144

Published: Sept. 30, 2023

The present study was intended to optimize the culture conditions for secondary metabolite production by endophytic fungi. Based on morphology and phylogeny, fungus identified as Trichoderma longibrachiatum isolated from brinjal leaf based morphological characterization. antifungal activity evaluated against phytopathogens such Macrophomina phaseolina, Phytopthora infestans, Colletotrichum falcatum gloeosporioides through overlapping method, filtrate organic fraction Potato dextrose both a growth medium. exhibited significant activity, while modifications in medium composition may possess major impact quantity quality of metabolites production. To achieve maximum production, optimized with screening basal media, carbon, nitrogen, pH, trace elements incubation period. final fermentation were Minimal ereavis broth media; glucose sucrose carbon source; Peptone Yeast extract nitrogen source, sodium nitrate precursor; pH 6; period 7 days at 28°C. This optimization resulted 47.19-60.67% M. P. C. falcatum, which higher than that before (43.80%). GCMS revealed distinct T. longibrachiatum, comprising molecules additional bioactivities. These results strengthen ongoing research disease control agriculture emphasizing biocontrol potential phyllosphere plant pathogenic

Language: Английский

Citations

0