In Vitro Anticancer Effects of Aqueous Leaf Extract from Nepeta nuda L. ssp. nuda DOI Creative Commons
Zlatina Gospodinova,

G Antov,

Svetozar Stoichev

et al.

Life, Journal Year: 2024, Volume and Issue: 14(12), P. 1539 - 1539

Published: Nov. 24, 2024

Despite significant efforts, cancer remains the second leading cause of mortality worldwide. The medicinal plant Nepeta nuda L. represents a valuable source biologically active compounds with pharmacological activities including antioxidant, anti-inflammatory, antimicrobial, and antiviral. This study aimed to assess antiproliferative potential mechanisms action aqueous extract from leaves wild-grown N. nuda. Cancer cell lines, MDA-MB-231, MCF7 (breast), HT29, Colon 26 (colon), HepG2 (liver cancer), non-cancerous skin line, BJ, were assessed for activity by MTT assay observation morphological alterations. line that was most sensitive further studied apoptotic alterations Annexin V/propidium iodide staining, colony-forming assay, qRT-PCR analysis. results revealed inhibited proliferation all investigated lines strongest cytostatic effect on cells half maximal inhibitory concentration (IC50) value 380.2 μg/mL selectivity index (SI) 3.5. significantly ability form colonies, exhibited considerable proapoptotic involving participation CASP8 gene, increased expression levels ATG3 BECN1 which suggests role autophagic death in antitumor action.

Language: Английский

Via Air or Rhizosphere: The Phytotoxicity of Nepeta Essential Oils and Malus Dihydrochalcones DOI Creative Commons
Slavica Dmitrović, Jasmina Nestorović Živković, Dijana Smailagić

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(5), P. 701 - 701

Published: Feb. 25, 2025

Many specialized metabolites found in plants have significant potential for developing environmentally friendly weed management solutions. This review focuses on the phytotoxic effects of volatile terpenes and phenolic compounds, particularly nepetalactone, an iridoid monoterpenoid from Nepeta species, phloretin, a dihydrochalcone predominantly genus Malus. We highlight current findings their herbicidal effects, including morphological, physiological, biochemical responses target plants. These results underscore sustainable herbicides that could control weeds with minimal environmental impact. also discuss soil persistence methods to enhance solubility, chemical stability, bioavailability. Additionally, possible non-target organisms, such as pollinators, non-pollinating insects, microbiota, are considered. However, further research deeper understanding long-term ecological impact, along resistance development risk assessment, is essential bioherbicides be applied practices.

Language: Английский

Citations

0
Plant iridoids: Chemistry, dietary sources and potential health benefits DOI Creative Commons
Liangchuan Guo,

Jinli Qiao,

Junwei Huo

et al.

Food Chemistry X, Journal Year: 2025, Volume and Issue: 27, P. 102491 - 102491

Published: April 1, 2025

Iridoids, a diverse class of plant food monoterpenoids, are characterized by cyclopentane-fused pyran ring structure and exhibit extensive structural diversity functional versatility. This review highlights recent advances in iridoid chemistry, biosynthesis via the methylerythritol phosphate pathway, advanced extraction techniques such as ultrasound-assisted, microwave-assisted, supercritical fluid extraction. Analytical methods liquid chromatography-mass spectrometry enable precise identification quantification, advancing study their health-promoting properties. Iridoids demonstrate potent antioxidant, anti-inflammatory, neuroprotective, antitumor, antiviral, hepatoprotective effects suggesting potential use foods, nutraceuticals, pharmaceuticals, cosmetics. However, for successful commercialization iridoid-based products, future research should aim at cost-effective production iridoids using sustainable systems, biotechnological synthesis, clinical validation. reveals significant promise enhancing human health through product innovation assessment.

Language: Английский

Citations

0
Transient expression of PRISEs and Trichoderma-mediated elicitation promote iridoid production in Nepeta sibirica L. DOI Creative Commons
Neda Aničić, Dragana Matekalo, Dejan Stojković

et al.

Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 109986 - 109986

Published: May 1, 2025

Language: Английский

Citations

0
Patterns of Genetic Variation of Nepeta nuda L. from the Central Balkans: Understanding Drivers of Chemical Diversity DOI Creative Commons
Luka Petrović, Marijana Škorić, Branislav Šiler

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(11), P. 1483 - 1483

Published: May 27, 2024

Nepeta nuda L., a notable medicinal species in the tradition of Balkan region, is rich source bioactive iridoids and phenolics previously described as high-resolution taxonomical classifiers for genus Nepeta. However, their potential investigating intra-species differentiation here first time. The aim was to recognize sources natural chemical diversity association with genetic variability both within among N. populations Central Balkans. Chemical assessed from methanol extracts essential oils through untargeted targeted metabolomics using state-of-the-art analytical tools, covering broad spectrum compounds that represent metabolome. We found chemodiversity primarily resides nuda, similar results were obtained at DNA level microsatellite markers. low studied implies metabolomic profiles may be less influenced by geographic distance variable environmental conditions Balkans, they are under pivotal control backgrounds. Screening distribution major belonging (phenolic acids flavonoids) (both aglycones glycosylated forms), populations, able guarantee mass spectrometry-based tools selection elite representative genotypes practical importance. knowledge acquired will allow us delve deeper into molecular background diversity, which course our further work.

Language: Английский

Citations

3
Cytokinins enhance the metabolic activity of in vitro-grown catmint (Nepeta nuda L.) DOI Creative Commons
Miroslava Zhiponova, Zhenya Yordanova, Anna Zaharieva

et al.

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 214, P. 108884 - 108884

Published: June 27, 2024

The phytohormones cytokinins are essential mediators of developmental and environmental signaling, primarily during cell division endophytic interactions, among other processes. Considering the limited understanding regulatory mechanisms that affect growth bioactivity medicinal plant Nepeta nuda (Lamiaceae), our study aimed to explore how influence plant's metabolic status. Exogenous administration active cytokinin forms on in vitro N. internodes stimulated intensive callus formation de novo shoot regeneration, leading a marked increase biomass. This process involved an accumulation oxidants, which were scavenged by peroxidases using phenolics as substrates. tissue formed upon addition 6-benzylaminopurine (BAP) acted sink for sugars allocation nutrients between culture medium regenerated plants. In accordance, significantly enhanced content polar metabolites their respective biological activities compared untreated wild-grown BAP-mediated major phenolic metabolites, rosmarinic acid (RA) caffeic (CA), corresponded with variations expression levels genes biosynthesis. contrast, iridoids corresponding biosynthetic not affected. conclusion, elucidated mechanism action demonstrated its potential stimulating production bioactive compounds. knowledge could serve basis further investigations impact productivity.

Language: Английский

Citations

1
Molecular background of the diverse metabolic profiles in leaves and inflorescences of naked catmint (Nepeta nuda L.) DOI Creative Commons
Luka Petrović, Biljana Filipović, Marijana Škorić

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 28, 2024

L. shares a typical secondary chemistry with other

Language: Английский

Citations

1
In Vitro Anticancer Effects of Aqueous Leaf Extract from Nepeta nuda L. ssp. nuda DOI Creative Commons
Zlatina Gospodinova,

G Antov,

Svetozar Stoichev

et al.

Life, Journal Year: 2024, Volume and Issue: 14(12), P. 1539 - 1539

Published: Nov. 24, 2024

Despite significant efforts, cancer remains the second leading cause of mortality worldwide. The medicinal plant Nepeta nuda L. represents a valuable source biologically active compounds with pharmacological activities including antioxidant, anti-inflammatory, antimicrobial, and antiviral. This study aimed to assess antiproliferative potential mechanisms action aqueous extract from leaves wild-grown N. nuda. Cancer cell lines, MDA-MB-231, MCF7 (breast), HT29, Colon 26 (colon), HepG2 (liver cancer), non-cancerous skin line, BJ, were assessed for activity by MTT assay observation morphological alterations. line that was most sensitive further studied apoptotic alterations Annexin V/propidium iodide staining, colony-forming assay, qRT-PCR analysis. results revealed inhibited proliferation all investigated lines strongest cytostatic effect on cells half maximal inhibitory concentration (IC50) value 380.2 μg/mL selectivity index (SI) 3.5. significantly ability form colonies, exhibited considerable proapoptotic involving participation CASP8 gene, increased expression levels ATG3 BECN1 which suggests role autophagic death in antitumor action.

Language: Английский

Citations

0