Exploring the potential of seed inoculation with microbial consortia to mitigate drought stress in maize plants under greenhouse conditions

Plant and Soil, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Abstract Background and aims Plant-beneficial microbes may attenuate climate change-induced stresses on plants such as drought. We investigated the potential of beneficial microbial consortia (BMc) plant growth rhizosphere bacterial/archaeal community under Methods Seeds Zea mays B73 were inoculated with six plant-beneficial bacterial isolates either alone or combined in two three-member (BMc1, BMc2) before sowing loamy sandy substrates greenhouse. A known effective consortium (BMc3) was included positive control. Drought treatment established BMc treatments by omitting watering last five weeks period. The maize single determined. Colony-forming units (CFUs) inoculants evaluated selective plating, effects native assessed using 16S rRNA gene amplicon sequencing basal root tip grown loam. Results In both water conditions, CFUs inoculations higher at roots than tips. Under well-watered seed inoculation a isolate had no effect substrates. resulted shoot (but not root) compared to non-inoculated controls conditions zone most important driver for beta-diversity, followed while showed effect. Conclusion Our study suggests that has drought stress during growth.

Language: Английский

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Testing plant growth promoting microorganisms in the field - a proposal for standards

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 16, 2024

In the European Union and worldwide there are a burgeoning markets for plant growth promoting microorganisms (PGPM) other biological agents as soil improvers, bio-fertilizers, bio-stimulants, control or bio-pesticides. Microbial have major share in this development. The use of such products is often advertised with promise contributing to sustainable agricultural practices by increasing crop yield offering an alternative substitute decrease dependency agriculture on hazardeous agrochemicals. contrast registered microbial protection products, PGPM that marketed EU improvers biostimulants, not strictly required proven minimum efficacy levels under field conditions. Manufacturers only ensure these do pose unacceptable risks human, animal health, safety environment. Uniform guidelines comparable EPPO - standards (European Mediterranean Plant Protection Organisation) test trials available. This paper attempts fill gap. It proposes trial design implementation, well recommendations type scope data collection evaluation. Selected research papers from literature were evaluated analyze, whether what extent requirements already met. majority had clear experimental followed proper Frequent deficiencies low number tested environments species, insufficient site agronomic management description missing humidity temperature. Using suggested assumed increase expressive power products.

Language: Английский

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Combined use of humic acids and Trichoderma harzianum as sustainable alternatives to alliviate salt stress in bell pepper

Brazilian Journal of Biology, Journal Year: 2025, Volume and Issue: 85

Published: Jan. 1, 2025

Abstract Bell pepper is a vegetable with beneficial properties for human nutrition. However, salinity an abiotic factor affecting bell yield in arid and semi-arid areas of Peru. The objective was to determine the combined effect humic acid (HA) Trichoderma harzianum (TH) as sustainable alternatives increase peppers subjected salt stress. experiment carried field out during 2023 2024 seasons design 2 x 3 factorial scheme, referring electrical conductivity (EC) irrigation water (0 4 dSm-1), HA (0, 15 30 L ha-1) TH 1 kg ha-1). Agronomic yield-related parameters were analyzed. results showed that saline soil accompanied by irrigations 0 dS m-1 EC, significant decrease agronomic concentration sodium chlorine leaves. application ha-1 plant height (86.53 y 86,42%), higher root dry weight (95.44 95.32%) (90.46 90.41%), also, greatly improved fruit length 64.6 63.74%) width (58.47 57.31%), significantly increased content per (91.49 91.30%), (93.29 93.15%) total (89.54 89.23%) relation plants It also increases potassium calcium leaves (37.88 48.71%), K+/ Na+ ratio (72.07 74.93%) proline (58.60 59.31%) superior control, defense mechanism against Therefore, dose acids increased, shows tolerance salinity, being alternative reduce stress

Language: Английский

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Inoculation of wild type or EPS mutant Bacillus subtilis does not alter rhizocompetence, soil EPS, and tomato growth in the absence of stress

Rhizosphere, Journal Year: 2025, Volume and Issue: unknown, P. 101076 - 101076

Published: April 1, 2025

Language: Английский

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Selective recruitment of beneficial microbes in the rhizosphere of maize affected by microbial inoculants, farming practice, and seasonal variations

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Background Plant beneficial microorganisms as inoculants can improve crop performance, but factors affecting their impact on plant performance under field conditions remain unclear, thereby limiting use in farming. Here, we investigated how farming practices (e.g. tillage and N-fertilization intensity) growing seasons influenced the of a microorganism consortium (BMc: Trichoderma, Bacillus, Pseudomonas strains) maize affected rhizosphere competence each BMc strain. In addition, tested whether affects resident microbiome performance. two (2020 2021), assessed inoculation growth, nutritional status, gene expression, different at flowering stage. Results Inoculated strains successfully colonized independent practice. improved growth iron uptake 2020, regardless These effects co-occurred with lower precipitation levels 2020 compared to 2021. reduced expression several stress-related genes for drought. An increased by BMc-inoculated plants was observed associated upregulation ZmNAS3, which is linked uptake. Therefore, mitigated drought maize. The microbial communities were altered both years, patterns responder taxa differed between seasons. Metagenome analysis revealed that more encoding biosurfactants siderophores) enriched than Moreover, identified bacterial fungal positively relative abundance these significantly due while they showed overall higher abundances 2021, inoculation. We mapped sequences publicly available genomes verified occurrence various traits genomes. Conclusions Overall, show season determined effect shaping composition function findings highlight importance complex interplay abiotic stress conditions.

Language: Английский

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Trichoderma consortium compost corncob promotes the growth of Pseudostellaria heterophylla and alleviates its diseases incidence via reshaping the soil microbiome and improving the soil nutrients under continuous monocropping conditions

Industrial Crops and Products, Journal Year: 2024, Volume and Issue: 216, P. 118800 - 118800

Published: May 21, 2024

Language: Английский

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Rhizosphere competent inoculants modulate the apple root–associated microbiome and plant phytoalexins

Applied Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 108(1)

Published: May 27, 2024

Abstract Modulating the soil microbiome by applying microbial inoculants has gained increasing attention as eco-friendly option to improve disease suppressiveness. Currently, studies unraveling interplay of inoculants, root-associated microbiome, and plant response are lacking for apple trees. Here, we provide insights into ability Bacillus velezensis FZB42 or Pseudomonas sp. RU47 colonize microhabitats modulate their microbiome. We applied two strains plants grown in soils from same site either affected replant (ARD) not (grass), screened establishment selective plating, measured phytoalexins roots 3, 16, 28 days post inoculation (dpi). Sequencing 16S rRNA gene ITS fragments amplified DNA extracted dpi different microhabitat samples revealed significant effects on fungal β-diversity root-affected rhizoplane. Interestingly, only ARD soil, most abundant bacterial amplicon sequence variants (ASVs) changed significantly relative abundance. Relative abundances ASVs affiliated with Enterobacteriaceae were higher rhizoplane reduced both inoculants. Bacterial communities root endosphere but presence was indicated. Interestingly previously unobserved, responded increased phytoalexin content roots, more pronounced grass than soil. Altogether, our results indicate that rhizosphere competent, modulated perceived plants, which could make them interesting candidates an mitigation strategy ARD. Key points • Rhizosphere competent (mainly fungi) Inoculants abundance stronger

Language: Английский

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Shift in the soil rhizobacterial community for enhanced solubilization and bioavailability of phosphorus in the rhizosphere of Allium hookeri Thwaites, through bioaugmentation of phosphate-solubilizing bacteria

3 Biotech, Journal Year: 2024, Volume and Issue: 14(8)

Published: July 27, 2024

Language: Английский

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Roots of resilience: Optimizing microbe‐rootstock interactions to enhance vineyard productivity

Plants People Planet, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 18, 2024

Societal Impact Statement Grape production relies signifcantly on agrochemicals, such as fertilizers and pesticides, to sustain vine health yield. However, excessive or improper use of these inputs leads detrimental environmental effects, including soil degradation, water contamination, biodiversity decline. To address this, research must explore sustainable alternatives. Enhancing the symbiotic interactions between grapevine rootstocks beneficial microorganisms offers a viable pathway. By fostering natural relationships, producers scientists can develop environmentally viticulture practices that strengthen resilience without compromising productivity. This approach also supports broader transition regenerative, ecologically balanced agricultural systems. Summary The productivity vineyards are influenced by complex surrounding microbiome. Emerging has highlighted pivotal role microbe‐rootstock alliances in modulating nutrient acquisition, water‐use efficiency, pathogen resistance. leveraging potential microorganisms, viticulturists optimize vineyard management enhance overall productivity, stability, sustainability. Through strategic selection with enhanced mycorrhizal associations targeted introduction plant growth‐promoting rhizobacteria, growers support adapting challenging conditions. Aditionally, manipulating rhizosphere microbiome, through techniques biofertilization reverse microdialysis, foster development robust, disease‐suppressive communities safeguard vines against biotic abiotic stressors. Integrating microbiome‐centric approaches into comprehensive strategies, potentially future rootstock genetic improvement programs, holds promise for improving grape yield, quality, long‐term viticultural

Language: Английский

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Microbial inoculants modulate the rhizosphere microbiome, alleviate plant stress responses and enhance maize growth at field scale

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 30, 2024

Background Field inoculation of crops with beneficial microbes is a promising sustainable strategy to enhance plant fitness and nutrient acquisition. However, effectiveness can vary due environmental factors, microbial competition, methodological challenges, while their precise modes action remain uncertain. This underscores the need for further research optimize strategies consistent agricultural benefits. Results Using comprehensive, multidisciplinary approach, we investigated effects consortium (BMc) (Pseudomonas sp. RU47, Bacillus atrophaeus ABi03, Trichoderma harzianum OMG16) on maize (cv. Benedictio) through an experiment conducted within long-term field trial across intensive extensive farming practices. Additionally, unexpected early drought stress emerged as climatic variable, offering insight into consortium. Our findings demonstrate that BMc primarily enhanced growth fitness, particularly by increasing iron uptake, which crucial adaptation. Inoculated plants showed improved shoot compared non-inoculated plants, regardless Specifically, modulated hormonal balance, detoxification reactive oxygen species (ROS), increased root exudation iron-chelating metabolites. Amplicon sequencing revealed shifts in rhizosphere bacterial fungal communities mediated consortium, metagenomic shotgun indicated enrichment genes related antimicrobial lipopeptides siderophores. Conclusions highlight multifaceted benefits significantly influencing metabolism, responses, microbiome. These improvements are advancing practices enhancing resilience productivity.

Language: Английский

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