Fungi Associated with Olive Tree (cv. ‘Nocellara del Belice’) Decay in Trapani Province (Sicily, Italy) DOI Creative Commons
Marika Lamendola, Giulia Mirabile,

Josè Muratore

et al.

Pathogens, Journal Year: 2024, Volume and Issue: 13(11), P. 932 - 932

Published: Oct. 25, 2024

Recently, in several locations the province of Trapani (Sicily, Italy), olive growers have reported cases decaying trees cv. ‘Nocellara del Belice’, showing symptoms defoliation, branch drying, xylem browning, and reduced production. Internal include white brown wood rot, starting from base trunk. These alterations been observed irrigated using a pipe system at trunk with spray sprinklers. To identify causal agents decay, some were eradicated dissected, woody samples processed to isolate associated fungal micro-organisms. The most common colonies identified morphological (macro- microscopical observation) molecular (PCR amplification rDNA-ITS region) analyses. Nine taxa identified, which four this decay syndrome (Coriolopsis gallica, Fomitiporia mediterranea, Kirschsteiniothelia sp., Pleurostoma richardsiae), three considered ubiquitous opportunistic fungi (Alternaria spp., Aspergillus amstelodami, Trichoderma sp.), other two mycelia sterilia. Artificial inoculation satisfied Koch’s postulates, confirming pathogenicity aforementioned fungi, even though infections fields seem be related irrigation system. This hypothesis would confirmed by progression over time subjected described but not groves differently managed. It is therefore appropriate conduct further more in-depth investigations aimed studying correlation between system, presence agents, manifestation syndrome. A ongoing investigation use biostimulants (Agrusaver, Savory Sun, VA LLC) on symptomatic trees, aim both improving vegetative performance host limiting detected field.

Language: Английский

Groundbreaking Technologies and the Biocontrol of Fungal Vascular Plant Pathogens DOI Creative Commons
Carmen Gómez‐Lama Cabanás, Jesús Mercado‐Blanco

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(1), P. 77 - 77

Published: Jan. 18, 2025

This review delves into innovative technologies to improve the control of vascular fungal plant pathogens. It also briefly summarizes traditional biocontrol approaches manage them, addressing their limitations and emphasizing need develop more sustainable precise solutions. Powerful tools such as next-generation sequencing, meta-omics, microbiome engineering allow for targeted manipulation microbial communities enhance pathogen suppression. Microbiome-based include design synthetic consortia transplant entire or customized soil/plant microbiomes, potentially offering resilient adaptable strategies. Nanotechnology has advanced significantly, providing methods delivery biological agents (BCAs) compounds derived from them through different nanoparticles (NPs), including bacteriogenic, mycogenic, phytogenic, phycogenic, debris-derived ones acting carriers. The use biodegradable polymeric non-polymeric eco-friendly NPs, which enable controlled release antifungal while minimizing environmental impact, is explored. Furthermore, artificial intelligence machine learning can revolutionize crop protection early disease detection, prediction outbreaks, precision in BCA treatments. Other genome editing, RNA interference (RNAi), functional peptides efficacy against pathogenic fungi. Altogether, these provide a comprehensive framework management diseases, redefining modern agriculture.

Language: Английский

Citations

2
Assessment of Peacock Spot Disease (Fusicladium oleagineum) in Olive Orchards Through Agronomic Approaches and UAV-Based Multispectral Imaging DOI Creative Commons

Hajar Hamzaoui,

Ilyass Maafa, Hasnae Choukri

et al.

Horticulturae, Journal Year: 2025, Volume and Issue: 11(1), P. 46 - 46

Published: Jan. 6, 2025

Olive leaf spot (OLS), caused by Fusicladium oleagineum, is a significant disease affecting olive orchards, leading to reduced yields and compromising tree health. Early accurate detection of this critical for effective management. This study presents comprehensive assessment OLS progression in orchards integrating agronomic measurements multispectral imaging techniques. Key parameters—incidence, severity, diseased area, index—were systematically monitored from March October, revealing peak values 45% incidence April 35% severity May. Multispectral drone imagery, using sensors NIR, Red, Green, Red Edge spectral bands, enabled the calculation vegetation indices. Indices incorporating near-infrared such as SR705-750, exhibited strongest correlations with (correlation coefficients 0.72 0.68, respectively). combined approach highlights potential remote sensing early supports precision agriculture practices facilitating targeted interventions optimized orchard The findings underscore effectiveness traditional advanced analysis improve surveillance promote sustainable cultivation.

Language: Английский

Citations

1
Pitting the olive seed microbiome DOI Creative Commons
Nuria M. Wentzien, Antonio J. Fernández‐González, Antonio Valverde‐Corredor

et al.

Environmental Microbiome, Journal Year: 2024, Volume and Issue: 19(1)

Published: March 15, 2024

Abstract Background The complex and co-evolved interplay between plants their microbiota is crucial for the health fitness of plant holobiont. However, seeds still relatively unexplored no studies have been conducted with olive trees so far. In this study, we aimed to characterize bacterial, fungal archaeal communities present in ten genotypes growing same orchard through amplicon sequencing test whether genotype a major driver shaping seed microbial community, identify origin latter. Therefore, developed methodology obtaining samples from seed’s endosphere under sterile conditions. Results A diverse was uncovered seeds, being an important factor influencing structure composition communities. most abundant bacterial phylum Actinobacteria , accounting average relative abundance 41%. At genus level, Streptomyces stood out because its potential influence on community structure. Within Basidiomycota Ascomycota were phyla, including genera Malassezia Cladosporium Mycosphaerella . shared microbiome composed four ( Stenotrophomonas Promicromonospora Acidipropionibacterium ) three genera. Furthermore, comparison findings obtained here earlier results root indicated that such as both compartments. Conclusions This study provides first insights into microbiota. highly reflect unique signature clearly shaped although found. We identified may translocate roots they organs trees. These set stage future research vertical transmission endophytes role specific taxa germination, development, seedling survival.

Language: Английский

Citations

5
Olive Tree Belowground Microbiota: Plant Growth-Promoting Bacteria and Fungi DOI Creative Commons
Maria Celeste Dias, Sónia Silva, Cristina Galhano

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(13), P. 1848 - 1848

Published: July 5, 2024

The olive tree is one of the most significant crops in Mediterranean region. Its remarkable adaptability to various environments has facilitated cultivation across diverse regions and agricultural scenarios. rising global demand for products, coupled with climate challenges, driving changes methods. These are altering traditional landscape may potentially reshape structure composition orchard microbial communities, which can impact productivity stress tolerance. Bacterial fungal communities naturally associated plants have long been recognized as crucial plant growth health, serving a vital component sustainable agriculture. In this review, we aim highlight significance abiotic stresses. We update current knowledge on profiles rhizosphere root bacterial orchards examine how (a)biotic factors influence these communities. Additionally, explore potential growth-promoting bacteria fungi enhancing physiological performance identify gaps emphasize need implementing new strategies. A comprehensive understanding olive-associated microbiota will aid developing agronomic practices address climatic challenges meet growing products.

Language: Английский

Citations

5
Potential Therapeutic Properties of Olea europaea Leaves from Selected Cultivars Based on Their Mineral and Organic Profiles DOI Creative Commons
Natália M. de Oliveira, Jorge Machado,

Maria Helena Chéu

et al.

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(3), P. 274 - 274

Published: Feb. 22, 2024

Olive leaves are consumed as an extract or a whole herbal powder with several potential therapeutic benefits attributed to polyphenols, tocopherol’s isomers, and flavonoids, among others. This study assessed the variance in functional features presented by olive from three different Portuguese cultivars—Cobrançosa, Madural, Verdeal—randomly mix-cultivated geographical area of Vale de Salgueiros. Inorganic analysis determined their mineral profiles while organic measured total phenolic flavonoid content, scanned tocopherol fatty acid composition. The extracts’ biological activity was tested determining antimicrobial antioxidant power well ability inhibit acetylcholinesterase, butyrylcholinesterase, MAO-A/B, angiotensin-I-converting enzyme. inorganic showed them be inexpensive source able address deficiencies. All cultivars appear have for use possible antioxidants future alternative antibiotics against some multidrug-resistant microorganisms, caution regarding arsenic content Verdeal cultivar. Madural’s displayed properties considered natural multitarget treatment Alzheimer’s Parkinson’s diseases, depression, cardiometabolic dual blood pressure modulation. work indicates that randomly cultivating significantly modifies leaves’ composition keeping multifaceted value.

Language: Английский

Citations

4
Enhancing Abiotic Stress Resilience in Mediterranean Woody Perennial Fruit Crops: Genetic, Epigenetic, and Microbial Molecular Perspectives in the Face of Climate Change DOI Open Access
Aliki Kapazoglou, Eleni Tani, Vasileios Papasotiropoulos

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3160 - 3160

Published: March 29, 2025

Enhanced abiotic stresses such as increased drought, elevated temperatures, salinity, and extreme weather phenomena severely affect major crops in the Mediterranean area, a ‘hot spot’ of climate change. Plants have evolved mechanisms to face stressful conditions adapt environmental pressures. Intricate molecular processes involving genetic epigenetic factors plant–microbe interactions been implicated response tolerance stress. Deciphering whereby plants perceive respond stress is crucial for developing strategies counteract challenges. Progress determining genes, complex gene networks, biochemical pathways, well plant–microbiota crosstalk, involved has achieved through application tools diverse resources. This knowledge could be particularly useful accelerating plant improvement generating resilient varieties, especially concerning woody perennial crops, where classical breeding lengthy labor-intensive process. Similarly, understanding provide insights into innovative approaches facing conditions. In this review, we comprehensive overview discuss recent findings genetic, epigenetic, microbial aspects shaping responses, context enhancing resilience important fruit crops.

Language: Английский

Citations

0
Evaluation of the Biostimulant Activity and Verticillium Wilt Protection of an Onion Extract in Olive Crops (Olea europaea) DOI Creative Commons
Ana Falcón-Piñeiro,

Javier Zaguirre-Martínez,

Ana Claudia Ibáñez-Hernández

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(17), P. 2499 - 2499

Published: Sept. 6, 2024

The olive tree is crucial to the Mediterranean agricultural economy but faces significant threats from climate change and soil-borne pathogens like Verticillium dahliae. This study assesses dual role of an onion extract formulation, rich in organosulfur compounds, as both biostimulant antifungal agent. Research was conducted across three settings: a controlled climatic chamber with non-stressed trees; experimental farm trees under abiotic stress; two commercial orchards affected by V. Results showed that chamber, significantly reduced MDA levels leaves, more pronounced reduction observed when applied irrigation compared foliar spray. treatment also increased root length up 37.1% controls. In field trials, number new shoots 148% 53.5%. orchards, treated exhibited levels, lower dahliae density, 26.7% increase fruit fat content. These findings suggest effectively reduces oxidative stress pathogen colonization, while enhancing plant development supports use formulation promising, sustainable alternative chemical treatments for improving crop resilience.

Language: Английский

Citations

1
Water and nutrient availability modulate the salinity stress response in Olea europaea cv. Arbequina DOI Creative Commons
Marzia Vergine, Emily Palm, Anna Maria Salzano

et al.

Plant Stress, Journal Year: 2024, Volume and Issue: unknown, P. 100648 - 100648

Published: Oct. 1, 2024

Language: Английский

Citations

1
Rhizobacterial diversity of Portuguese olive cultivars in the Douro valley and their potential as plant growth promoters DOI
Isabel N. Sierra-García,

Maria J. Ferreira,

Camille Eichelberger Granada

et al.

Journal of Applied Microbiology, Journal Year: 2024, Volume and Issue: 135(9)

Published: Aug. 23, 2024

Abstract Aims This study investigated the bacterial communities in rhizosphere of two traditional Portuguese olive cultivars, Cobrançosa and Negrinha de Freixo, relation to soil properties. Additionally, we aimed isolate identify bacteria with potential for biocontrol other plant growth-promoting traits from these communities. Methods results Bacterial were using a metabarcoding approach physicochemical properties groves also analyzed. Higher richness was associated Freixo growing high organic matter content water-holding capacity. In contrast, soils grove presented higher pH electric conductivity. enriched ASVs (Amplicon Sequence Variants) belonging Bacillus, Gaiella, Acidothermus, Bradyrhizobium, uncultured Xanthobacteraceae. On hand, characterized by relative abundance Streptomyces Sphingomonas. isolation screening activities performed. Six strains, predominantly Bacillus isolated demonstrated antagonistic against fungal pathogen Colletotrichum gloeosporoides growth promotion (PGP) traits. Conclusions Our findings demonstrate that structure trees is shaped both cultivar soil-related factors. The number species related greater isolates traits, particularly strains.

Language: Английский

Citations

0
Fungi Associated with Olive Tree (cv. ‘Nocellara del Belice’) Decay in Trapani Province (Sicily, Italy) DOI Creative Commons
Marika Lamendola, Giulia Mirabile,

Josè Muratore

et al.

Pathogens, Journal Year: 2024, Volume and Issue: 13(11), P. 932 - 932

Published: Oct. 25, 2024

Recently, in several locations the province of Trapani (Sicily, Italy), olive growers have reported cases decaying trees cv. ‘Nocellara del Belice’, showing symptoms defoliation, branch drying, xylem browning, and reduced production. Internal include white brown wood rot, starting from base trunk. These alterations been observed irrigated using a pipe system at trunk with spray sprinklers. To identify causal agents decay, some were eradicated dissected, woody samples processed to isolate associated fungal micro-organisms. The most common colonies identified morphological (macro- microscopical observation) molecular (PCR amplification rDNA-ITS region) analyses. Nine taxa identified, which four this decay syndrome (Coriolopsis gallica, Fomitiporia mediterranea, Kirschsteiniothelia sp., Pleurostoma richardsiae), three considered ubiquitous opportunistic fungi (Alternaria spp., Aspergillus amstelodami, Trichoderma sp.), other two mycelia sterilia. Artificial inoculation satisfied Koch’s postulates, confirming pathogenicity aforementioned fungi, even though infections fields seem be related irrigation system. This hypothesis would confirmed by progression over time subjected described but not groves differently managed. It is therefore appropriate conduct further more in-depth investigations aimed studying correlation between system, presence agents, manifestation syndrome. A ongoing investigation use biostimulants (Agrusaver, Savory Sun, VA LLC) on symptomatic trees, aim both improving vegetative performance host limiting detected field.

Language: Английский

Citations

0