BMC Genomics,
Journal Year:
2024,
Volume and Issue:
25(1)
Published: May 14, 2024
Carotenoid
cleavage
oxygenases
(CCOs)
enzymes
play
a
vital
role
in
plant
growth
and
development
through
the
synthesis
of
apocarotenoids
their
derivative.
These
chemicals
are
necessary
for
flower
fruit
coloration,
as
well
manufacture
hormones
such
abscisic
acid
(ABA)
strigolactones,
which
control
variety
physiological
processes.
The
CCOs
gene
family
has
not
been
characterized
Arachis
hypogaea.
Genome
mining
A.
hypogaea
identifies
24
AhCCO
members.
was
divided
into
two
subgroups
based
on
recent
study
Arabidopsis
thaliana
CCO
classification
system.
Twenty-three
genes,
constituting
95.8%
total,
were
regulated
by
29
miRNAs,
underscoring
significance
microRNAs
(miRNAs)
governing
expression
peanuts.
AhCCD19
is
only
that
lacks
miRNA
target
site.
physicochemical
characteristics
genes
molecular
weights
isoelectric
points
studied
further.
then
regarding
chromosomal
distribution,
structure,
promoter
cis-elements.
Light,
stress
development,
drought
stress,
hormone
responsiveness
discovered
to
be
associated
with
can
utilized
developing
more
resilient
crops.
investigation
also
showed
cellular
location
encoded
proteins
peanut
carotenoid
oxygenase
family's
expansion
most
likely
result
tandem,
segmental,
whole-genome
duplication
events.
localization
expresses
abundance
mostly
cytoplasm
chloroplast.
Expression
analysis
shows
AhCCD7
AhCCD14
show
maximum
apical
meristem,
lateral
leaf,
pentafoliate
leaf
while
AhNCED9
AhNCED13
express
response
Aspergillus
flavus
resistance.
This
knowledge
throws
light
evolutionary
history
may
help
researchers
better
understand
processes
behind
occurrences
plants.
An
integrated
synteny
used
find
orthologous
hypogaea,
whereas
Beta
vulgaris
references
functional
characterization
genes.
studies
provide
foundation
future
research
regulation
functions
this
family.
information
provides
valuable
insights
genetic
technology
could
create
markers
breeding
programs
develop
new
lines.
BMC Genomic Data,
Journal Year:
2024,
Volume and Issue:
25(1)
Published: July 19, 2024
Abstract
The
coffee
industry
holds
importance,
providing
livelihoods
for
millions
of
farmers
globally
and
playing
a
vital
role
in
the
economies
coffee-producing
countries.
Environmental
conditions
such
as
drought
temperature
fluctuations
can
adversely
affect
quality
yield
crops.Carotenoid
cleavage
oxygenases
(CCO)
enzymes
are
essential
plants
they
help
break
down
carotenoids
contributing
to
growth
stress
resistance.
However,
knowledge
about
CCO
gene
family
Coffee
arabica
was
limited.
In
this
study
identified
21
genes
(C.
arabica)
revealing
two
subfamilies
carotenoid
dioxygenases
(
CCDs)
9-cis-epoxy
NCED
)
through
phylogenic
analysis.
These
exhibited
distribution
patterns
terms
structure,
domains,
motifs.
CaCCO
genes,
comprising
5
16
CCD
were
found
across
chromosomes.
Promoter
sequencing
analysis
revealed
cis-elements
that
likely
interact
with
plant
stress-responsive,
growth-related,
phytohormones,
like
auxin
abscisic
acid.
A
comprehensive
genome-wide
comparison,
between
C.
A.
thaliana
conducted
understand
characteristics
genes.
RTqPCR
data
indicated
CaNCED5,
CaNCED6,
CaNCED12,
CaNCED20
target
involved
leading
increased
crop
yield,
conditions,
limited
water
availability.
This
reveals
CCOs
responding
abiotic
identifies
potential
useful
breeding
stress-resistant
varieties.
Plant Stress,
Journal Year:
2024,
Volume and Issue:
13, P. 100538 - 100538
Published: July 19, 2024
This
study
investigates
how
plants
protect
themselves,
specifically
looking
at
a
group
of
genes
called
Xyloglucan
Endotransglucosylase/Hydrolase
(XTH)
in
cucumber
plants.
A
detailed
investigation
CsXTH
genes,
uncover
their
evolutionary
background,
structural
motifs,
regulatory
networks,
and
functional
responsibilities.
The
utilized
various
bioinformatics
tools
databases
to
highlight
the
dynamic
interplay
between
abiotic
stress
responses.
begins
with
phylogenetic
analysis
that
reveals
cladding
into
four
categories.
Further
unraveling
conserved
motif
domain
analyses
expose
coordination
xyloglucan
modification,
highlighting
essential
motifs
domains
shared
among
these
genes.
Analyzing
cis-regulatory
elements
helps
us
understand
complex
networks
control
defense
mechanisms
Among
elements,
CGTCA-motif
TGACG-motif
stand
out
as
key
players
interpreting
environmental
signals
coordinating
microRNA
Csa-miR169
is
becoming
player
regulating
expression
controlling
biological
responses
Chromosome
mapping,
Ka/Ks
calculations,
protein-protein
interaction
offer
insights
dynamics
Additionally,
explores
sub-cellular
localization,
uncovering
diverse
cellular
compartments
where
are
active.
Finally,
through
chrysophanol
physcion,
we
unveil
adaptable
functions
specific
response
distinct
treatments,
underscoring
role
orchestrating
cucumber's
adaptive
strategies.
Plant Breeding,
Journal Year:
2024,
Volume and Issue:
143(5), P. 675 - 694
Published: July 1, 2024
Abstract
In
the
present
study,
aim
was
to
predict
and
validate
putative
candidate
genes
underlying
drought‐tolerant
quantitative
trait
loci
(QTLs)
in
rice
crop
using
silico
approaches
real‐time
polymerase
chain
reaction
(RT‐PCR).
The
major
QTLs
which
have
been
reported
by
data
mining,
sequence
variation,
gene
ontology
analysis,
traits
finder
expression
analysis
were
subjected
RiceVarmap
software
design
primers,
only
a
few
variants
gave
SNP/InDel
primers;
thus,
finally,
15
primers
ultimately
selected,
used
identification
of
differentially
expressed
(DEGs)
among
contrasting
genotypes
IR
64
N
22
for
drought
tolerance
RT‐PCR
studies
providing
stress
treatment
during
panicle
initiation
stage.
this
investigation,
we
predicted
11
as
QTLs.
Out
these,
four
found
responsible
effect
regions
such
QTL‐Qsn‐4b,
QTL‐rn7a,
QTL‐Qtgw‐2a
QTL‐phc4.1
prioritized
candidates
identified
that
leaf
tissues.
Only
belong
two
QTLs,
primer
vg0712623096
from
QTL‐rn7a
(LOC_Os07g22450)
located
on
chromosome‐7
encoding
NAC
domain‐containing
protein
vg0431750843(LOC_Os04g53310)
soluble
starch
synthase
3‐
chloroplast
precursor,
vg0432626757
(LOC_Os04g54850)
pectin
acetylesterase
vg0433031562
(LOC_Os04g55520)
AP2
protein,
chromosome‐4
higher
differential
comparison
with
per
2
–ΔΔCt
values.
Considering
overall
these
primers/genes
genomic
governing
tolerance.
Therefore,
could
be
focussed
further
functional
exploit
breeding.
