A 2-oxoglutarate-dependent dioxygenase, GLUCORAPHASATIN SYNTHASE 1 (GRS1) is a major determinant for different aliphatic glucosinolates between radish and Chinese cabbage

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 13, 2024

Glucosinolates (GSLs) are secondary metabolites in Brassicaceae plants and play a defensive role against variety of abiotic biotic stresses. Also, it exhibits anti-cancer activity cancer cell human. Different profiles aliphatic GSL compounds between radish Chinese cabbage were previously reported. However, molecular details underlying the divergent profile two species not clearly understood. In this study, we found that major difference GSLs is determined by dominantly expressed genes first step modification phase, which responsible for enzymatic catalysis methylthioalkyl-glucosinolate. For instance, active expression GLUCORAPHASATIN SYNTHASE 1 (GRS1) gene an important production glucoraphasatin (GRH) glucoraphenin (GRE), radish. Meanwhile, was to merely produce (GRH), instead producing glucoraphanin (GRA) gluconapin (GNP) due mere GRS1 homologs abundant expressions FLAVIN-CONTAINING MONOOXYGENASES (FMO GS-OX) cabbage. addition, noticed wounding treatment on leaf tissues substantially enhanced indolic both radish, indicating wound-induced plants.

Language: Английский

Optimizing Light Intensity and Salinity for Sustainable Kale (Brassica oleracea) Production and Potential Application in Marine Aquaponics

Sustainability, Journal Year: 2024, Volume and Issue: 16(23), P. 10516 - 10516

Published: Nov. 30, 2024

With rising populations and increasing food consumption, the demand for is placing significant strain on freshwater resources. Exploring crops that can thrive under saline conditions crucial to ensuring security. Although brackish seawater abundant, it generally unsuitable irrigation. However, some plants exhibit tolerance moderate levels of salinity. This study investigated effects varying light intensities (150 250 photosynthetic photon flux densities) salinity (<1.5, 5, 10, 17 parts per thousand, equivalent <26, 86, 171, 291 millimolars) growth nutrient composition Russian kale (Brassica oleracea) grown in indoor hydroponics. The experiment was conducted over five months, from September 2023 January 2024. results revealed a intensity PPFD <1.5–5 ppt (<26–86 mM) were optimal maximizing biomass yield kale, whereas reduction observed at exceeding 10 (171 mM). In contrast, dry matter percentage significantly higher (291 macronutrient contents, particularly total Kjeldahl nitrogen (TKN), phosphorus (TP), magnesium (Mg), consistent across both (150–250 PPFDs) between <1.5 (<26–171 but reduced micronutrient concentrations, such as those copper (Cu), iron (Fe), zinc (Zn), lower PPFD) levels. These findings suggest optimizing essential enhancing nutritional value environments. outcomes are vital improving agricultural productivity resilience salt-affected regions, thereby supporting broader security sustainability goals.

Language: Английский

A 2-oxoglutarate-dependent dioxygenase, GLUCORAPHASATIN SYNTHASE 1 (GRS1) is a major determinant for different aliphatic glucosinolates between radish and Chinese cabbage

Plant Molecular Biology, Journal Year: 2024, Volume and Issue: 115(1)

Published: Dec. 10, 2024

Language: Английский