Leucine rich repeat-malectin receptor kinases IGP1/CORK1, IGP3 and IGP4 are required for arabidopsis immune responses triggered by β-1,4-D-Xylo-oligosaccharides from plant cell walls

The Cell Surface, Journal Year: 2024, Volume and Issue: 11, P. 100124 - 100124

Published: April 4, 2024

Pattern-Triggered Immunity (PTI) in plants is activated upon recognition by Pattern Recognition Receptors (PRRs) of Damage- and Microbe-Associated Molecular Patterns (DAMPs MAMPs) from or microorganisms, respectively. An increasing number identified DAMPs/MAMPs are carbohydrates plant cell walls microbial extracellular layers, which perceived PRRs, such as LysM Leucine Rich Repeat-Malectin (LRR-MAL) receptor kinases (RKs). LysM-RKs (e.g. CERK1, LYK4 LYK5) needed for fungal MAMP chitohexaose (β-1,4-D-(GlcNAc)6, CHI6), whereas IGP1/CORK1, IGP3 IGP4 LRR-MAL RKs required perception β-glucans, like cellotriose (β-1,4-D-(Glc)3, CEL3) mixed-linked glucans. We have explored the diversity Arabidopsis thaliana seedlings determining PTI responses treatment with different oligosaccharides polysaccharides. These analyses revealed that xylans [β-1,4-D-(xylose)4 (XYL4)], glucuronoxylans α-1,4-glucans, polysaccharides seaweeds activate PTI. Cross-elicitation experiments XYL4 other glycans showed mechanism DAMP 33-α-L-arabinofuranosyl-xylotetraose (XA3XX) shares some features CEL3 but differs CHI6. Notably, XA3XX impaired igp1/cork1, igp3 igp4 mutants, almost not affected cerk1 lyk4 lyk5 triple mutant. conserved species since pre-treatment triggers enhanced disease resistance tomato to Pseudomonas syringae pv DC3000 wheat. results expand triggering immunity support relevance activation. The characterization immune mechanisms involved carbohydrate-based structures recognized further understand modulation. show here DAMPs β-1,4-D-(xylose)4 (XYL4) (XA3XX), expanding function these glycan

Language: Английский

---

Genome-wide identification, characterization, and expression analysis of Clavata3 Insensitive receptor Kinases (CIKs) on developmental tissues and under temperature stress conditions in Brassica oleracea

Journal of Plant Biochemistry and Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 20, 2025

Language: Английский

---

Signaling of Plant Defense Mediated by Receptor-like Kinases, Receptor-like Cytoplasmic Protein Kinases and MAPKs Triggered by Fungal Chitin in Horticultural Crops

Horticulturae, Journal Year: 2024, Volume and Issue: 10(4), P. 361 - 361

Published: April 5, 2024

Fresh horticultural products are economically significant foods that highly demanded by consumers worldwide; however, they perishable and susceptible to deterioration fungi, which contribute their short shelf-life cause post-harvest losses. Among the alternatives suggested for fungal control in plants is elicitation of innate plant defense mechanism, may be activated when specific molecules phytopathogen, such as chitin, recognized. Chitin a long-chain polymer N-acetyl-α-D-glucosamine cell wall; it possesses biological activity eliciting immune response. This molecule its oligosaccharides recognized through transmembrane receptors known receptor-like kinases (RLKs) proteins (RLPs). Mediated cytoplasmic (RLCKs), bind intracellular domain these receptors, initiate signal transduction via MAP kinases, triggering In model plants, Oryza sativa (rice) Arabidopsis thaliana, set RLK/RLP-RLCK-MAP involved immunity triggered chitin. Furthermore, products, research into molecular events between three elements has similar processes occur. However, little about fruits. Against this background, present review provides most recent relevant findings on associations response chitin outlines could participate signaling process crops.

Language: Английский

---

Identification of cysteine-rich receptor-like kinase gene family in potato: revealed StCRLK9 in response to heat, salt and drought stresses

Functional Plant Biology, Journal Year: 2024, Volume and Issue: 51(6)

Published: May 9, 2024

The investigation into cysteine-rich receptor-like kinases (CRLKs) holds pivotal significance as these conserved, upstream signalling molecules intricately regulate fundamental biological processes such plant growth, development and stress adaptation. This study undertakes a comprehensive characterisation of CRLKs in Solanum tuberosum (potato), staple food crop immense economic importance. Employing comparative genomics evolutionary analyses, we identified 10 distinct CRLK genes potato. Further categorisation three major groups based on sequence similarity was performed. Each member potato systematically named according to its chromosomal position. Multiple alignment phylogenetic analyses unveiled conserved gene structures motifs within the same groups. genomic distribution observed across Chromosomes 2–5, 8 12. Gene duplication analysis highlighted noteworthy trend, with most pairs exhibiting Ka/Ks ratio greater than one, indicating positive selection StCRLKs Salt drought stresses significantly impacted peroxidase catalase activities seedlings. presence diverse cis-regulatory elements, including hormone-responsive underscored their involvement myriad biotic abiotic responses. Interestingly, interactions between phytohormone auxin proteins potential auxin-mediated regulatory mechanism. A holistic approach combining transcriptomics quantitative PCR validation StCRLK9 candidate involved response heat, salt stresses. lays robust foundation for future research functional roles family potatoes, offering valuable insights mechanisms applications management.

Language: Английский

---

Analysis of the CRK expressions in bottle gourd (Lagenaria siceraria) under Fusarium oxysporum f. sp. lagenariae stress by using genome-wide identification strategy

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: March 3, 2025

The cysteine-rich receptor-like kinases (CRKs) family in plants have been reported to perform multiple functions against various stresses. However, the CRK bottle gourd (Lagenaria siceraria) has not well-explored. Herein, a comprehensive genome-wide identification and characterization of gene carried out under Genome-wide genes gourds Fusarium oxysporum f. sp. lagenariae infection. A stringent set bioinformatic analyses identified 18 LsCRKs genome. Chromosomal mapping revealed that were distributed 4 chromosomes phylogenetic analysis divided them into two subgroups on tree. synteny collinearity among themselves other plant CRKs provided insights their conservancy expansion. Gene ontology suggested possible roles regulating different physiological processes stress responses gourd. To assess involvement F. infection, seedlings transplanted pots with oxysporum-infected soil. expression exhibited induced expression, suggesting gourd-F. interactions. Additionally, protein-protein interaction some important interacting partners crucial present work explored analyzed Functional predictions network modulating dynamics LsCKRs fungal pathogen infection suggest response gourds. Overall, results study provide basic information about response.

Language: Английский

---

AtSERK1 and BAK1/AtSERK3 positively regulate seed germination in response to saline condition in Arabidopsis

Biochemical and Biophysical Research Communications, Journal Year: 2025, Volume and Issue: unknown, P. 151682 - 151682

Published: March 1, 2025

Language: Английский

---

Male Germ Cell Specification in Plants

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(12), P. 6643 - 6643

Published: June 17, 2024

Germ cells (GCs) serve as indispensable carriers in both animals and plants, ensuring genetic continuity across generations. While it is generally acknowledged that the timing of germline segregation differs significantly between ongoing debates persist new evidence continues to emerge. In this review, we delve into studies focusing on male germ cell specifications summarize core gene regulatory circuits specification, which show remarkable parallels those governing meristem homeostasis. The similarity establishment plants also discussed.

Language: Английский

---

Moss-pathogen interactions: a review of the current status and future opportunities

Frontiers in Genetics, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 11, 2025

In complex and diverse environments, plants face constant challenges from various pathogens, including fungi, bacteria, viruses, which can severely impact their growth, development, survival. Mosses, representing early divergent lineages of land plants, lack traditional vascular systems yet demonstrate remarkable adaptability across habitats. While sharing the fundamental innate immune common to all mosses have evolved distinct chemical physical defense mechanisms. Notably, they exhibit resistance many pathogens that typically affect plants. Their evolutionary significance, relatively simple morphology, well-conserved mechanisms make excellent model organisms for studying plant-pathogen interactions. This article reviews current research on moss-pathogen interactions, examining host-pathogen specificity, characterizing infection phenotypes physiological responses, comparing pathogen susceptibility between angiosperms. Through this analysis, we aim deepen our understanding plant system evolution potentially inform innovative approaches enhancing crop disease resistance.

Language: Английский

---

TMK interacting network of receptor like kinases for auxin canalization and beyond

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 2, 2025

SUMMARY Receptor-like kinases (RLKs), particularly the Transmembrane Kinase (TMK) family, play essential roles in signaling and development, with TMKs being key components of auxin perception downstream phosphorylation events. While TMKs’ involvement canalization, a process for vasculature formation regeneration, has been established, nonetheless, additional regulatory partners remain poorly understood. In this study, we identify characterize seven leucine-rich repeat RLKs (TINT1–TINT7) as novel interactors TMK1, revealing their diverse evolutionary, structural, functional characteristics. Our results show that TINTs interact TMK1 highlight regulating various developmental processes. Majority contributes, together to TINT5 linking other canalization component CAMEL. Beyond also establish role TINT-TMK1 interactions processes such stomatal movement hypocotyl’s gravitropic response. These findings suggest TINTs, through interaction are integral networks, contributing both broader plant development.

Language: Английский

---

Genome-Wide Analysis of AGC Genes Related to Salt Stress in Soybeans (Glycine max)

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2588 - 2588

Published: March 13, 2025

The AGC protein kinase family plays a crucial role in regulating plant growth, immunity, and cell death, as well responses to abiotic stresses such salt-induced stress, which impact development productivity. While the functions of kinases have been thoroughly studied model plants Arabidopsis thaliana, their roles soybeans (Glycine max) remain poorly understood. In this study, we identified 69 genes soybeans, are unevenly distributed across 19 chromosomes classified into five subfamilies: PDK1, AGCVI, AGCVII, AGCVIII, (other). Each subfamily shares similar exon–intron structures specific motifs. Gene duplication selection pressure analyses revealed that GmAGC gene is primarily expanded through segmental or whole-genome duplication, with all undergoing purifying during evolution. Promoter analysis numerous cis-regulatory elements associated light, hormonal, stress responses, including salt stress. expression demonstrated tissue-specific patterns, highest levels found roots (19.7%). Among 54 analyzed using RT-qPCR, significant changes were observed leaves treated sodium chloride, most showing increased expression. These results illustrate critical soybean salinity Our findings suggest targeting may enhance resistance toxicity, offering valuable insights for future crop improvement strategies.

Language: Английский

---