Intercropping of Saccharum spp. with Dictyophora indusiata: effects on microbial communities and metabolite profiles during bagasse degradation

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 22, 2025

Background Intercropping Saccharum spp. (sugarcane) with the fungus Dictyophora indusiata together bagasse amendment represents an innovative circular agriculture method that can enhance soil health, boost sugarcane yields, and increase farm profitability. Understanding process by which D. degrades is key to optimizing this method. Aims This study aims clarify microbial metabolic processes involved in degradation intercropping system. Methods Chemical composition analysis, metabarcoding sequencing, metabolomic profiling were conducted on -degraded (DIBA) naturally degraded (BA). Results Analysis of chemical revealed only acid detergent fiber (ADF) crude protein content differed significantly between DIBA BA treatments. Metabarcoding sequencing showed altered bacterial fungal communities, reducing diversity. Metabolomic analysis indicated enhancement biological metabolism, particularly carbohydrate breakdown, treatment. Key metabolites, such as glucose, cellobiose, D-mannose, more abundant samples. In addition, unique metabolites L-alanine, serine, oxaloacetate detected treatment, suggesting efficient compared natural processes. Conclusion The use macrofungi play a pivotal role transforming agricultural waste into valuable amendments. Future research should focus long-term impact system quality crop yield, well underlying mechanisms, further optimize systems fungi management.

Language: Английский

Intercropping Shapes the Metabolome and Microbiome of Medicinal Giant Lily (Cardiocrinum giganteum) in Bamboo, Chinese Fir, and Mixed Forests

Forests, Journal Year: 2024, Volume and Issue: 15(12), P. 2201 - 2201

Published: Dec. 13, 2024

Intercropping is a promising strategy for sustainable medicinal plant cultivation, but its impact on plant–microbe interactions remains poorly understood. This study investigated the influence that intercropping giant lily (Cardiocrinum giganteum) with bamboo (BG), Chinese fir (FG), and mixed forests (MG) had metabolome microbiome compared to monoculture control (GG). Metabolomic analysis revealed BG significantly increased accumulation of terpenoids (e.g., yucalexin B22, 19.39-fold), alkaloids anabasine, 2.97-fold), steroids O-acetyl-lariciresinol, 4.49-fold), while MG induced production stress-related metabolites aflatoxin G2, 128.62-fold), FG enhanced nitrogen metabolism putrescine, 2.47-fold). altered rhizosphere endophytic microbial communities, enriching beneficial bacteria Acidobacteria Alphaproteobacteria) promoting symbiotic fungi Serendipita Xylariales). Network strong correlations between specific taxa Bacillus Ceratobasidiaceae) key norpandamarilactonine A, methylgingerol), indicating their potential roles in shaping metabolic profiles lily. These findings highlight complex interplay systems, provide basis developing targeted cultivation strategies enhance bioactive compounds other plants.

Language: Английский