Combined Bulked Segregant Analysis-Sequencing and Transcriptome Analysis to Identify Candidate Genes Associated with Cold Stress in Brassica napus L

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1148 - 1148

Published: Jan. 28, 2025

Cold tolerance in rapeseed is closely related to its growth, yield, and geographical distribution. However, the mechanisms underlying cold resistance remain unclear. This study aimed explore genes provide new insights into molecular of rapeseed. Rapeseed M98 (cold-sensitive line) D1 (cold-tolerant were used as parental lines. In their F2 population, 30 seedlings with lowest damage levels highest selected construct cold-tolerant cold-sensitive pools, respectively. The two pools lines analyzed using bulk segregant sequencing (BSA-seq). G’-value analysis indicated a single peak on Chromosome C09 candidate interval, which had 2.59 Mb segment 69 genes. Combined time-course weighted gene co-expression network analyses performed at seven time points reveal genetic basis two-parent response low temperatures. Twelve differentially expressed primarily involved plant identified. BSA-seq transcriptome revealed BnaC09G0354200ZS, BnaC09G0353200ZS, BnaC09G0356600ZS Quantitative real-time PCR validation was consistent RNA-seq. facilitates exploration

Language: Английский

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Integrating Phenotypic and Molecular Approaches to Unravel Salinity and Cold Tolerance in Wetland Plants for Ecosystem Restoration

Environmental and Experimental Botany, Journal Year: 2025, Volume and Issue: unknown, P. 106108 - 106108

Published: Feb. 1, 2025

Language: Английский

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Changes of Catalase and Peroxidase Activity and Expression Under Cold Stress in Prunus persica Cultivars with Different Cold Tolerances

Agronomy, Journal Year: 2025, Volume and Issue: 15(3), P. 556 - 556

Published: Feb. 25, 2025

Peach is one of the most common stone fruit crops, but it also thermophilic. One main problems in peach cultivation frost up to −8 °C spring during pollen development, budding, and flowering. The adaptation plant low temperatures could be related activation antioxidant system under cold stress. aim this work was test hypothesis distinct mechanisms stress Prunus persica L. cultivars with various tolerances. difference between study previous ones that previously, only contrasting varieties (resistant sensitive) were studied. For first time, we studied effect on cold-resistant different degrees resistance, such as “Loadel” “Springold” (medium resistant) “Podarok Like” “Temisovskij” (highly resistant). experiment designed simulate effects short-term snaps, which are a occurrence February March south Crimea. A series tests conducted annual shoots frozen at −12 °C. activity gene expression two major enzymes, catalase peroxidase, by spectrophotometry RT-qPCR, respectively. showed these enzymes responded differently Catalase similarly all four varieties. After frost, there an increase (7-fold 3-fold Like”) decrease expression. In contrast catalase, peroxidase opposite response medium-tolerant highly tolerant cultivars. Peroxidase after exposure increased (1.5-fold “Temisovskij”), while decreased (1.5–2 fold “Loadel”, respectively). change opposite. It resistant medium-resistant Thus, our results revealed (peroxidase) moderately data obtained show high degree resistance have other involved, may useful for selecting

Language: Английский

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Genome-Wide Analysis of CPP Transcription Factor Family in Endangered Plant Phoebe bournei and Its Response to Adversity

Plants, Journal Year: 2025, Volume and Issue: 14(5), P. 803 - 803

Published: March 5, 2025

The CPP gene family comprises transcription factor genes containing a conserved CRC domain, which is mainly involved in plant development and evolution. Although have been widely studied many plants, little known about them woody especially the endangered species Phoebe bournei (Hemsl.). In genome of bournei, we identified 11 PbCPP (PbCPP1-PbCPP11) distributed on four chromosomes, with large differences number amino acids. They encode both acidic alkaline proteins. A phylogenetic analysis showed that these can be divided into three subfamilies, A, B, C, contain seven, two, two genes, respectively. Through an interspecific collinearity analysis, homologous genes. promoter cis-acting element revealed PbCPPs variety elements respond to hormones, stress signals, light play role growth development, most (except PbCPP3 PbCPP8) MYB binding site regulate drought-induced responses, indicating they important drought resistance. An expression PbCPP4 was high roots stems lower leaves, whereas other low roots, stems, leaves. addition, six representative were detected using qRT-PCR. results show significant under abiotic conditions (drought, cold, salt), responses. This study preliminarily verified different great significance for understanding its mechanism adaptation.

Language: Английский

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Divergence in cold stress response in yacon [Smallanthus sonchifolius (Poepp. and Endl.) H. Rob.] clones with different ploidy levels

South African Journal of Botany, Journal Year: 2025, Volume and Issue: 180, P. 751 - 761

Published: April 5, 2025

Language: Английский

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Regulatory role of ABA‐responsive element binding factors in plant abiotic stress response

Physiologia Plantarum, Journal Year: 2025, Volume and Issue: 177(2)

Published: March 1, 2025

Abstract As sessile organisms, plants are inevitably threatened by various abiotic stresses. Abiotic stresses seriously affect plant growth and development crop yield. Plants have evolved complex regulatory networks to resist that occur during their life cycle. The hormone abscisic acid (ABA) is accumulated under osmotic stress conditions such as drought, salt, others. ABA signaling pathway plays a key role in response stresses, which ABA‐responsive element binding factors (ABFs) play crucial the whole process. ABFs class of basic leucine zipper proteins (bZIPs) specifically recognize elements belong subfamily bZIP family. discoveries ABFs‐mediated tolerance multiple including salinity, cold, heat highlighted discussed this review. aim review further analyze mechanisms regulation mediated lay foundation for breeding superior stress‐resistant varieties.

Language: Английский

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Melatonin-mediated low-temperature tolerance of cucumber seedlings requires Ca2+/CPKs signaling pathway

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 214, P. 108962 - 108962

Published: July 22, 2024

Language: Английский

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Identification of Cold Tolerance Transcriptional Regulatory Genes in Seedlings of Medicago sativa L. and Medicago falcata L.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(19), P. 10345 - 10345

Published: Sept. 26, 2024

Alfalfa species Medicago sativa L. (MS) and falcata (MF), globally prominent perennial leguminous forages, hold substantial economic value. However, our comprehension of the molecular mechanisms governing their resistance to cold stress remains limited. To address this knowledge gap, we scrutinized compared MS MF cold-stress responses at level following 24 h 120 low-temperature exposure (4 °C). Our study revealed that had superior physiological resilience with MS, its morphology was healthier under stress, malondialdehyde content superoxide dismutase activity increased, first, then decreased, while soluble sugar continued accumulate. Transcriptome analysis showed after exposure, there were different gene-expression patterns between MF, including 1274 2983 genes continuously up-regulated, respectively, a total 923 included, star cold-resistant such as ICE1 SIP1. Gene ontology (GO) enrichment Kyoto Encyclopedia Genes Genomes (KEGG) pathway analyses numerous inter-species differences in sustained responses. Notably, MS-exclusive included single transcription factor (TF) gene several associated DNA repair-related pathway, whereas MF-exclusive comprised nine TF 14 pathways. Both exhibited high-level expression encoding TFs belonging AP2-EREBP, ARR-B, bHLH families, indicating potential roles sustaining alfalfa-related species. These findings provide insights into which could inform breeding programs aimed enhancing alfalfa cultivars.

Language: Английский

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γ-aminobutyric acid contributes to a novel long-distance signaling in figleaf gourd rootstock-induced cold tolerance of grafted cucumber seedlings

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 216, P. 109168 - 109168

Published: Sept. 30, 2024

Language: Английский

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RIP5 Interacts with REL1 and Negatively Regulates Drought Tolerance in Rice

Cells, Journal Year: 2024, Volume and Issue: 13(11), P. 887 - 887

Published: May 21, 2024

Improving the drought resistance of rice is great significance for expanding planting area and improving stable yield rice. In our previous work, we found that ROLLED AND ERECT LEAF1 (REL1) protein promoted enhanced tolerance to stress by eliminating reactive oxygen species (ROS) levels triggering abscisic acid (ABA) response. However, mechanism through which REL1 regulates removing ROS unclear. this study, identified interacting 5 (RIP5) it directly combines with in chloroplast. We RIP5 was strongly expressed ZH11 under drought-stress conditions, rip5-ko mutants significantly improved plants drought, whereas overexpression resulted greater susceptibility drought. Further investigation suggested negatively regulated decreasing content ascorbic (AsA), thereby reducing clearance. RNA sequencing showed knockout caused differential gene expression chiefly associated ascorbate aldarate metabolism. Furthermore, multiple experimental results suggest involved regulating inhibiting RIP5. Collectively, findings reveal importance inhibition affecting rice’s response stress. This work not only explains rice, but will also help improve

Language: Английский

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