Drought induced metabolic shifts and water loss mechanisms in canola: role of cysteine, phenylalanine and aspartic acid DOI Creative Commons
Raëd Elferjani,

Shankar Pahari,

Raju Soolanayakanahally

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 23, 2024

Drought conditions severely curtail the ability of plants to accumulate biomass due closure stomata and decrease photosynthetic assimilation rate. Additionally, there is a shift in plant’s metabolic processes toward production metabolites that offer protection aid osmoadaptation, as opposed those required for development growth. To limit water loss via non-stomatal transpiration, adjust load composition cuticle waxes, which act an additional barrier. This study investigates impact soil deficit on stomatal epicuticular losses, well adjustments two canola ( Brassica napus L.) cultivars—one drought-tolerant other drought-sensitive. Specifically, we examined effect drought treatment, involved reducing holding capacity 40%, levels cysteine, sucrose, abscisic acid (ABA) leaves both cultivars. Next, looked potential differences night, predawn, early morning transpiration rates wax response drought. A substantial rise leaf cysteine was observed cultivars drought, strong correlation found between ABA, conductance, indicating sulfur may play role controlling movement during stress. Attributes related CO 2 diffusion (stomatal mesophyll conductance) were different suggesting better management relations under stress by cultivar. Epicuticular waxes acting barrier against loss. Surprisingly, responded similarly (cysteine, ABA) they not reliable markers our test setup. However, higher level phenylalanine cultivar suggestive this amino important adaptation drier climates. Furthermore, multitrait genotype-ideotype distance index (MGIDI) revealed likely aspartic sustaining nitrogen carbon immediate resumption after episodes. In conclusion, leveraging knowledge agriculture can enhance crop yield bolster resistance environmental challenges.

Language: Английский

Integrative Multi-Omics Approaches for Identifying and Characterizing Biological Elements in Crop Traits: Current Progress and Future Prospects DOI Open Access

Bing-Liang Fan,

L. CHEN, Lingling Chen

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1466 - 1466

Published: Feb. 10, 2025

The advancement of multi-omics tools has revolutionized the study complex biological systems, providing comprehensive insights into molecular mechanisms underlying critical traits across various organisms. By integrating data from genomics, transcriptomics, metabolomics, and other omics platforms, researchers can systematically identify characterize elements that contribute to phenotypic traits. This review delves recent progress in applying approaches elucidate genetic, epigenetic, metabolic networks associated with key plants. We emphasize potential these integrative strategies enhance crop improvement, optimize agricultural practices, promote sustainable environmental management. Furthermore, we explore future prospects field, underscoring importance cutting-edge technological advancements need for interdisciplinary collaboration address ongoing challenges. bridging this aims provide a holistic framework advancing research plant biology agriculture.

Language: Английский

Citations

2
A simple and cost-effective SNP genotyping assay for marker-assisted selection of wilt resistance in castor breeding DOI Creative Commons

Sathishkumar Ramalingam,

Manmode Darpan Mohanrao,

S. Geethanjali

et al.

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 226, P. 120693 - 120693

Published: Feb. 18, 2025

Language: Английский

Citations

1
Agro-physiological and transcriptome profiling reveal key genes associated with potato tuberization under different nitrogen regimes in aeroponics DOI Creative Commons

Rasna Zinta,

Jagesh Kumar Tiwari,

Tanuja Buckseth

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(3), P. e0320313 - e0320313

Published: March 28, 2025

Nitrogen (N) is a crucial nutrient for the growth and development of potatoes. However, excessive use nitrogen fertilizers can have detrimental effects on human health, aquatic ecosystems, environment. Therefore, understanding genes involved in metabolism essential developing future strategies to improve efficiency (NUE) plants. This study aimed identify associated with high tuber yield two contrasting potato varieties Kufri Jyoti (N inefficient) Pukhraj efficient) grown under low regimes using an aeroponics system. Both were doses (low N: 0.5 mM N; 5 N) completely randomized design (CRD) three replications over years. The phenotypic results confirmed that was more efficient compared Jyoti, particularly conditions. Additionally, transcriptome analysis produced high-quality data ( ≥ Q20), ranging from 4.35 5.46 Gb per sample. Statistically significant p ≤ 0.05) identified based reference genome. Differentially expressed (DEGs) categorized as either up-regulated or down-regulated leaf tissues. Transcriptome profiling both tissues revealed traits contributing DEGs further characterized through gene ontology (GO) annotation KEGG pathway analysis. Selected validated real-time quantitative polymerase chain reaction (RT-qPCR) In summary, several being component potatoes different These included glutaredoxin, transcription factors (BTB/POZ, AP2/ERF, MYB), nitrate transporter, aquaporin TIP1;3, glutamine synthetase, aminotransferase, GDSL esterase/lipase, sucrose synthase, UDP-glycosyltransferases, osmotin, xyloglucan endotransglucosylase/hydrolase, laccases. we overexpressed including cysteine protease inhibitor 1, miraculin, sterol desaturase, pectinesterase N stress. Our highlights these genes’ roles enhancing cultivated aeroponics.

Language: Английский

Citations

0
Exploring genomic loci and candidate genes associated with drought tolerance indices in spring wheat evaluated under two levels of drought DOI Creative Commons

Mohamed Mosalam,

Rahma A. Nemr, Maha Aljabri

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 1, 2025

Wheat is a major global crop, and increasing its productivity essential to meet the growing population demand. However limited water resources primary constraint. This study aimed identify genetic factors associated with drought tolerance using diverse panel of 287 wheat genotypes evaluated under well-watered drought-stressed conditions. Water Use Efficiency (WUE) Grain Yield (GY), along indices, were assessed. A genome-wide association (GWAS) 26,814 high-density SNP markers identified loci linked these traits, 768 SNPs showing significant associations. Additionally, genomic selection (GS) was performed rrBLUP model predict trait performance across environments. Among measured traits at -log10 (P) ≥ 3, 81 mapped higher threshold 4, indicating pleiotropic QTL-by-environment interaction effects. Several novel known genes, previously reported have functions related biotic abiotic stresses response SNPs. indices evaluated, stress index (STI), geometric mean (GMP), (TOL) most reliable indicators for selecting stable, high-yielding control The same three exhibited high prediction values severe (SS) condition. Five as promising candidates breeding programs based on their superior tolerance, grain yield, nutritional value. provides valuable insights into basis in wheat, highlighting key regions improved use efficiency yield stability. findings contribute development drought-tolerant varieties optimized utilization achieve increased per unit levels, ultimately contributing sustainable agriculture food security.

Language: Английский

Citations

0
Genome-wide association study uncovers key genomic regions governing agro-morphological and quality traits in Indian mustard [Brassica juncea (L.) Czern. and Coss.] DOI Creative Commons
Manoj Kumar Patel, Navinder Saini, Yashpal Taak

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(4), P. e0322120 - e0322120

Published: April 24, 2025

In Indian mustard, improving agro-morphological and quality traits through conventional methods are both cumbersome resource-intensive. Marker-aided breeding presents a promising solution to these challenges. Hence, the present research aimed identify genomic regions governing using genome-wide association studies (GWAS). The GWAS panel comprised 142 diverse genotypes of mustard were evaluated for 20 different traits, revealing significant difference among genotypes. Subsequently, genotyped Brassica 90K SNP array (Illumina). Structure diversity analysis grouped into 3 sub-populations or groups, LD decay 1.05 Mb was confirmed genotypic analysis. BLINK model revealed total 49 marker-trait associations (MTAs), in which 28 21 MTAs observed during rabi 2020–21 2021–22 various respectively. Amongst them, twelve demonstrated stable with studied including days 50% flowering (DF), 100% flower termination (DFT), maturity (DM), plant height (PH), main shoot length (MSL), siliqua (SL), seeds per (SPS), oil content (OC), glucosinolates (Glu) years. Moreover, silico nearby SNPs their 31 candidate genes known be involved molecular, physiological, biochemical pathways relevant traits. These can further characterized deciphered more precise utilization programs future.

Language: Английский

Citations

0
Morphological characteristics and genome-wide association analysis among local Andrographis paniculata from Thailand under controlled environment in plant factory DOI Creative Commons
Praderm Wanichananan,

Supattana Janta,

Suchalee Sueachuen

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(4), P. e0320667 - e0320667

Published: April 29, 2025

Andrographis paniculata Wall. ex Nees ( A. ) is a medicinal plant widely used in Southeast Asian traditional medicine. Plant factories with artificial lighting (PFAL) provide controlled environments for optimizing growth and quality. However, the variability biomass bioactive compound production among varieties cultivated PFAL not well understood. This study investigated ten locals of accessions to assess their characteristics andrographolide (AP1) content system using hydroponic cultivation. Among accessions, TTT cultivar showed significantly higher stem height, width, above-ground yield, leaf number, AP1 compared others. Phylogenetic analyses based on SNP markers revealed that morphologically distinct but genetically similar CR, RB, PL, PC accessions. A genome-wide association (GWAS) identified two significant regions chromosome 9 associated yield content. These findings highlight potential as high-quality pharmaceutical use insights into key genes could be targeted breeding programs improve systems.

Language: Английский

Citations

0
Genome-wide association study (GWAS) combined with transcriptome analysis reveals the key genes underlying the production of seed oil, mono and poly-unsaturated fatty acids in Brassica napus DOI Creative Commons
Maryam Salami,

Helin Tan,

William J. W. Thomas

et al.

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 231, P. 121205 - 121205

Published: May 21, 2025

Language: Английский

Citations

0
From wild to domesticated: exploring selection signatures caused by domestication in Carica papaya DOI
Mónica I. Jiménez-Rojas,

Pedro Jesús Ruiz-Gil,

Mauricio Heredia-Pech

et al.

Ecological Genetics and Genomics, Journal Year: 2025, Volume and Issue: unknown, P. 100367 - 100367

Published: May 1, 2025

Language: Английский

Citations

0
Genome-wide association studies and transcriptome analysis reveal novel genes associated with freezing tolerance in rapeseed (Brassica napus L.) DOI Creative Commons

Guoqiang Zheng,

Zigang Liu, Lixi Jiang

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(5), P. e0322547 - e0322547

Published: May 27, 2025

Freezing stress is the main obstacle affecting geographical distribution, growth, development, quality, and productivity of rapeseed ( Brassica napus ) in northern China. However, there a little knowledge freezing tolerance mechanism. Here, 289 core germplasms collected from 36 countries were used to identify SNPs associated with tolerance. We RNA-seq data narrow down candidate genes identified by genome-wide association studies. The frequency distributions phenotypic values best linear unbiased estimates (BLUE) for each trait conform normal or approximately distributions, good repeatability across various locations. results showed that 594, 513, 7, 45 significantly malondialdehyde, peroxidase, soluble protein, relative electrolyte leakage, respectively. Based on these SNPs, we 4,998 genes. Crossover analysis indicated 73 overlapped between GWAS datasets, 13 involved transmission perception freeze signals, lipid metabolism, reactive oxygen species (ROS) homeostasis, antifreeze proteins synthesis, other metabolic processes. These reveal novel rapeseed, provide basis further research improvement rapeseed.

Language: Английский

Citations

0
Meta-QTL analysis for mining of candidate genes and constitutive gene network development for viral disease resistance in maize (Zea mays L.) DOI Creative Commons
Mamta Gupta, Mukesh Choudhary, Alla Singh

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 11(1), P. e40984 - e40984

Published: Dec. 14, 2024

Language: Английский

Citations

2