Genome-wide analysis of Q binding reveals a regulatory network that coordinates wheat grain yield and grain protein content
Journal of genetics and genomics/Journal of Genetics and Genomics,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 1, 2025
Wheat
is
an
important
cereal
crop
used
to
produce
diverse
and
popular
food
worldwide
because
of
its
high
grain
yield
(GY)
protein
content
(GPC).
However,
GY
GPC
are
usually
negatively
correlated.
We
previously
reported
that
favorable
alleles
the
wheat
domestication
gene
Q
can
synchronously
increase
GPC,
but
underlying
mechanisms
remain
largely
unknown.
In
this
study,
we
investigated
regulatory
network
involving
associated
with
in
young
grains
through
DNA
affinity
purification
sequencing
transcriptome
analyses,
electrophoretic
mobility
shift
dual-luciferase
assays,
transgenic
approaches.
Three
Q-binding
motifs,
namely
TTAAGG,
AAACA[A/T]A,
GTAC[T/G]A,
were
identified.
Notably,
genes
related
photosynthesis
or
carbon
nitrogen
metabolism
enriched
regulated
by
Q.
Moreover,
was
revealed
bind
directly
own
glutamine
synthetase
TaGSr-4D
expression,
thereby
influencing
assimilation
during
filling
stage
increasing
GPC.
Considered
together,
our
study
findings
provide
molecular
evidence
positive
effects
on
Language: Английский
Integrative physiological and transcriptome analyses elucidate the effect of shading during the grain-filling stage of wheat (Triticum aestivum) cv. ZY96-3
Functional Plant Biology,
Journal Year:
2025,
Volume and Issue:
52(4)
Published: April 7, 2025
Shading
plays
an
important
role
in
determining
nutrient
content
and
yield
fo
wheat
(Triticum
aestivum).
However,
the
genetic
mechanism
underlying
effects
of
shading
treatment
on
grain
filling
remains
unclear.
Therefore,
we
performed
phenotypic
transcriptome
analyses
cv.
ZY96-3
during
development
under
normal
shaded
conditions.
resulted
a
significant
decrease
size
1000-grain
weight.
Correlation
analysis
revealed
strong
effect
mean
maximum
grain-filling
rate
secondary
parameters
R2
R3.
And
reduced
starch
starch-related
enzyme
activity
(including
granule-bound
synthase
soluble
synthase).
Transcriptomic
showed
that
mainly
affected
pathways
related
to
photosynthetic
antenna
proteins,
carbon
fixation
organisms,
sucrose
metabolism.
Sixteen
genes
protein
were
first
upregulated
then
downregulated;
whereas
all
differentially
expressed
(PetC,
Fd,
LFNR1,
LFNR2,
PC,
PsbO,
PsaG,
PSB28)
pathway
belonged
electron
transport
chain
proteins.
We
found
affects
physiological
molecular
properties
stage.
This
study
reveals
new
candidate
(such
as
TaLFNR1-7A
TaFd-7A)
for
breeding
varieties
with
high
efficiency
regions
insufficient
light
intensity.
Language: Английский
Dynamic Metabolomic Changes in the Phenolic Compound Profile and Antioxidant Activity in Developmental Sorghum Grains
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
73(2), P. 1725 - 1738
Published: Dec. 30, 2024
Phenolic
compounds
(PC)
were
analyzed
by
UHPLC-ESI-QTOF-MSE
in
two
sorghum
genotypes,
harvested
growing
seasons
(GS)
at
five
distinct
days
after
flowering
(DAF)
to
evaluate
how
genotype/GS
influences
the
PC
synthesis
and
antioxidant
capacity
during
grain
growth.
Total
phenolic
contents
strongly
correlated
with
(r
>
0.9,
p
<
0.05).
Globally,
97
annotated,
including
20
found
irrespective
of
developmental
stage
genotype/GS.
The
profile
clearly
differs
between
stages:
acids
most
abundant
class
early
stages
(50%),
flavonoid
accumulation
becomes
predominant
late
ones
(3/5
total
ion
abundance).
Dimeric
trimeric
tannins
identified
even
10DAF
grains.
Chemometry
revealed
great
variability
genotypes
(27%)
important
biomarkers
GS
differentiation
(e.g.,
ferulic
acid).
This
work
can
input
open
databases
paves
way
understand
biosynthetic
pathways
future
selection.
Language: Английский