Salinity‐Induced Photorespiration in Populus Vascular Tissues Facilitate Nitrogen Reallocation DOI
María del Mar Rubio Wilhelmi,

Nuchada Maneejantra,

Vimal Kumar Balasubramanian

et al.

Plant Cell & Environment, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

ABSTRACT Adaptation to abiotic stress is critical for the survival of perennial tree species. Salinity affects plant growth and productivity by interfering with major biosynthetic processes. Detrimental effects salinity may vary between different tissues cell types. However, spatial molecular mechanisms controlling responses are not yet thoroughly understood in trees. We used laser capture microdissection clones Populus tremula x alba isolate palisade vascular cells intermediary leaf from plants exposed 150 mM NaCl 10 days, followed a recovery period. Cell‐specific changes proteins metabolites were determined. induced vascular‐specific accumulation associated photorespiration, serine, 3‐phosphoglycerate NH 4 + suggesting N metabolism. Accumulation GLUTAMINE SYNTHETASE 2 protein, increased GS1.1 gene expression, indicated that produced photorespiration was assimilated glutamine, main amino acid translocated Further analysis total soluble stems roots showed bark storage treatments. Collectively, our results suggest salt‐induced mediates N‐reallocation , an essential process adaptation trees adverse conditions.

Language: Английский

Harnessing metabolomics for enhanced crop drought tolerance DOI Creative Commons
Ali Raza, Muhammad Anas, Savita Bhardwaj

et al.

The Crop Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

3
Mass spectrometry imaging in plants, microbes, and food: a review DOI Creative Commons

Mudita Vats,

Berta Cillero‐Pastor, Eva Cuypers

et al.

The Analyst, Journal Year: 2024, Volume and Issue: 149(18), P. 4553 - 4582

Published: Jan. 1, 2024

Plant health, which affects the nutritional quality and safety of derivative food products, is influenced by symbiotic interactions with microorganisms. These influence local molecular profile at tissue level. Therefore, studying distribution molecules within plants, microbes, plant-based crucial to assess plant ensure agricultural products that become part our supply, plan management practices. Within this framework, samples can be visualized mass spectrometry imaging (MSI). This review describes key MSI methodologies, highlighting role they play in unraveling localization metabolites, lipids, proteins, pigments, elemental components across products. Furthermore, investigations involve multimodal approaches combining other techniques are described. The advantages limitations different their applicability diverse agro-food studies described enable informed choices for tailored analyses. For example, some technologies meticulous sample preparation while others compromise spatial resolution gain throughput. Key parameters such as sensitivity, ionization bias fragmentation, reference database compound class specificity discussed review. With ongoing refinements instrumentation, data analysis, integration complementary techniques, deepens insight into biology ecosystem. turn empowers quest sustainable productive

Language: Английский

Citations

4
Spatial omics for accelerating plant research and crop improvement DOI
Rutwik Barmukh, Vanika Garg, Hao Liu

et al.

Trends in biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Salinity‐Induced Photorespiration in Populus Vascular Tissues Facilitate Nitrogen Reallocation DOI
María del Mar Rubio Wilhelmi,

Nuchada Maneejantra,

Vimal Kumar Balasubramanian

et al.

Plant Cell & Environment, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

ABSTRACT Adaptation to abiotic stress is critical for the survival of perennial tree species. Salinity affects plant growth and productivity by interfering with major biosynthetic processes. Detrimental effects salinity may vary between different tissues cell types. However, spatial molecular mechanisms controlling responses are not yet thoroughly understood in trees. We used laser capture microdissection clones Populus tremula x alba isolate palisade vascular cells intermediary leaf from plants exposed 150 mM NaCl 10 days, followed a recovery period. Cell‐specific changes proteins metabolites were determined. induced vascular‐specific accumulation associated photorespiration, serine, 3‐phosphoglycerate NH 4 + suggesting N metabolism. Accumulation GLUTAMINE SYNTHETASE 2 protein, increased GS1.1 gene expression, indicated that produced photorespiration was assimilated glutamine, main amino acid translocated Further analysis total soluble stems roots showed bark storage treatments. Collectively, our results suggest salt‐induced mediates N‐reallocation , an essential process adaptation trees adverse conditions.

Language: Английский

Citations

0