Unravelling the secondary metabolome and biocontrol potential of the recently described species Bacillus nakamurai

Microbiological Research, Journal Year: 2024, Volume and Issue: 288, P. 127841 - 127841

Published: Aug. 2, 2024

In the prospect of novel potential biocontrol agents, a new strain BDI-IS1 belonging to recently described Bacillus nakamurai was selected for its strong in vitro antimicrobial activities against range bacterial and fungal phytopathogens. Genome mining coupled with metabolomics revealed that produces multiple non-ribosomal secondary metabolites including surfactin, iturin A, bacillaene, bacillibactin bacilysin, together some ribosomally-synthesized post-translationally modified peptides (RiPPs) such as plantazolicin, potentially amylocyclicin, bacinapeptin LCI. Reverse genetics further showed specific involvement these compounds antagonistic activity strain. Comparative genomics between five already sequenced B. strains products constitute core metabolome species while RiPPs are more strain-specific. Although lacks key bioactive found velezensis, greenhouse experiments show is able protect tomato maize plants early blight northern leaf caused by Alternaria solani Exserohilum turcicum, respectively, at levels similar or better than velezensis QST713. The reduction foliar diseases, following root application suspension demonstrates can act direct antibiosis inducing plant defence mechanisms. These findings indicate be considered good candidate diseases prevailing tropical regions, encourage research into spectrum activity, requirements conditions needed ensure efficacy.

Language: Английский

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Increasing Application of Multifunctional Bacillus for Biocontrol of Pests and Diseases and Plant Growth Promotion: Lessons from Brazil

Agronomy, Journal Year: 2024, Volume and Issue: 14(8), P. 1654 - 1654

Published: July 27, 2024

The microbial genus Bacillus inhabits a diverse range of environments and is widespread across all global biomes, with significant presence in soil habitats. In agriculture, strains play multifaceted roles, serving as biocontrol agents against pests diseases, promoting plant growth by facilitating nutrient availability enhancing stress tolerance. Through mechanisms such phosphate solubilization, ACC-deaminase activity, synthesis phytohormones siderophores, spp. contribute to health crop productivity, new approach regenerative agriculture. ability solubilize makes essential nutrients more accessible plants, while activity helps plants withstand environmental stresses. Additionally, the can stimulate development, siderophores may facilitate uptake iron plants. As agricultural industry embraces Bacillus-based formulations for pest management enhancement, future research holds promising prospects optimizing their applications harnessing full potential agroecosystems. Continued exploration diversity interactions microbiota will further advance sustainable practices. This review contributes understanding how revolutionize agriculture health, increasing providing effective biological solutions diseases. successful application technologies millions hectares Brazilian demonstrates synergy between need practices use bio-inputs.

Language: Английский

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In vitro and In silico investigation deciphering novel antifungal activity of endophyte Bacillus velezensis CBMB205 against Fusarium oxysporum

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 3, 2025

Endophytes from medicinal plants are potential biocontrol agents against Fusarium oxysporum f. sp. cubense (Foc), which is the causative fungus of banana wilt disease. In present study, endophytic bacterium was isolated Globba racemosa and their antagonistic activities Foc were studied, probable molecular mechanism antagonism predicted by docking studies. The 16SrRNA sequencing confirmed isolate to be Bacillus velezensis CBMB205 (EG2). isolates distortion fungal hyphae illustrated in SEM. metabolites identified FTIR, suggesting presence C-H, CH3 O-H groups. Two major such as β-amyrin dihydroxy octadecenoic acid (DA) LC-MS analysis. Molecular studies suggested that these showed binding with chitin synthase 1 1,3-glucan pathogenic fungi. energy (BE) interaction between synthase-1 (CS-1), (1,3-GS) estimated -10.17 kcal/mol − 9.5 kcal/mol, respectively. BE CS-1 1,3-GS determined -2.43 3.4 current study demonstrated EG2 towards provided a silico also provides insight into developing metabolite-based antifungal for various agricultural applications.

Language: Английский

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Formation of a Novel Antagonistic Bacterial Combination to Enhance Biocontrol for Cucumber Fusarium Wilt

Microorganisms, Journal Year: 2025, Volume and Issue: 13(1), P. 133 - 133

Published: Jan. 10, 2025

Paenibacillus polymyxa strain PJH16, isolated and tested by our team, suppresses cucumber Fusarium wilt as an efficient biocontrol agent. For further investigation, the has been combined with two other Bacillus strains (Bacillus velezensis VJH504 subtilis JNF2) to enhance ability, which formed high-efficiency microbial agents in current study. The methodological target taken is based on achieving optimal growth conditions of agents; hence, medium composition culture were optimized through a single-factor test, orthogonal test response surface methodology. Following this, effectiveness combination was assessed pot experiments, provided theoretical foundation for synthesis flora significantly control wilt. results showed excellent compatibility, proving suitable proliferation VJH504, JNF2 together, specifically, when inoculation amounts adjusted 4% each. Using analysis, optimum composite identified 3% glucose carbon source, 2% yeast extract powder preferred nitrogen 1% dipotassium hydrogen phosphate most inorganic salt. Furthermore, optical density (OD600) solution reached its highest level at 3.16 under following conditions: volume 200 µL, 171 rpm speed, 21.6 h time, 30 °C cultural temperature, initial pH 7.0. experiment demonstrated that mixed bacterial achieved relative efficacy 93.4% against wilt, superior single- or pesticide treatment, also promoted growth. In summary, synthesized three displayed high concentration, optimization conditions, exerted remarkable growth-promoting effects This finding holds great significance future developments agents.

Language: Английский

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Bacillus Strains Isolated from Agroforestry Systems in the Amazon Promote Açaí Palm Seedling Growth When Inoculated as a Consortium

Agronomy, Journal Year: 2025, Volume and Issue: 15(3), P. 569 - 569

Published: Feb. 25, 2025

The hypothesis that cultivating açaí in agroforestry systems (AFS) can recruit beneficial microorganisms to its rhizosphere was tested this study. For purpose, rhizospheric soil samples were collected from an AFS area. colony-forming unit (CFU) counts of the 3.5 × 106 CFU/g, with no statistically significant differences between plants (p < 0.05). Regarding PGPR characteristics, 44 isolated strains, 18% produced siderophores, 9% mineralized organic phosphate, 15% solubilized inorganic and 7% indole-3-acetic acid (IAA) antimicrobial substances. Strains AP4-03, AP1-33, AP2-36 affiliated genus Bacillus sp. IAA at 1.45, 1.35, 2.02 µg/mL, respectively. Furthermore, these strains able inhibit growth fungus Pestalotiopsis by 69%, 67%, 71%, antifungal activity bacterial extracts, inhibition zones 23 mm (AP-03), 20 (AP-33), 18 (AP-36), 96% 92% 50 mg/mL (AP4-03 AP1-33) 100% 41 (AP2-36), observed. Considering seedling germination, palms inoculated strain AP1-33 differed controls terms root length hypocotyl length. treatments or all consortium when only compared control. Thus, analyzed showed potential improve initial development plants.

Language: Английский

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Modulation of the Sporulation Dynamics in the Plant-Probiotic Bacillus velezensis 83 via Carbon and Quorum-Sensing Metabolites

Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 26, 2025

Abstract Spore-forming Bacilli, such as the plant-associated Bacillus velezensis strains, are widely used probiotics, known for their safety and substantial health benefits both animal plant species. Through differentiation pathways mediated by quorum-sensing metabolites (QSMs), these bacteria develop multiple isogenic subpopulations with distinct phenotypes ecological functions, including motile cells, matrix-producing / cannibalistic competent spores, others. However, heterogeneity in populations is a significant limitation development of spore-based nutrients supplied during fermentation consumed through non-target pathways. One generation overflow (OMs), acetoin 2,3-butanediol. This study elucidates, using 2 3 full factorial experimental design, individual effects OMs, QSMs, interactions on sporulation dynamics subpopulation distribution B. 83. The results showed that OMs play relevant role external reserves carbon energy vitro nutrient scenarios, significantly affecting dynamics. improve efficiency reduce cell autolysis, but they also decrease cellular synchronization extend period spore formation. Although QSMs increase synchronization, desynchronization caused cannot be mitigated even addition autoinducer QSM pro-sporulation molecules, competence stimulating factor “CSF” cyclic lipopeptides. Indeed, interaction effect between displays antagonism efficiency. Modulating levels potential strategy regulating within cultures .

Language: Английский

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Discovery of antimicrobial peptides from Bacillus genomes against phytopathogens with deep learning models

Chemical and Biological Technologies in Agriculture, Journal Year: 2025, Volume and Issue: 12(1)

Published: March 9, 2025

Language: Английский

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Bacillus Genotypes Exhibit Antagonistic Effects on Lettuce-Based Enterobacter Pathotypes

Current Microbiology, Journal Year: 2025, Volume and Issue: 82(4)

Published: March 9, 2025

Language: Английский

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Biochemistry and differential mechanistic activity of antimicrobial lipopeptides from plant pathogen antagonists from the genus Bacillus

Canadian Journal of Plant Pathology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 13

Published: Nov. 29, 2024

Bacteria of the Bacillus genus produce various antimicrobial compounds that are effective against several plant pathogens. These strains proven pathogen antagonists, which cyclic lipopeptides (CLP) as a key group compounds. Fengycins, iturins and surfactins three main families CLP, share similar structure an oligopeptide ring attached to lipid tail. The proposed direct mechanisms activity for CLP their effects on membranes bacterial fungal typically result in changes cell membrane structure, leading pore formation, increased permeability and/or other effects. exact interaction with biomembranes resulting outcomes dependent family present. differential pathogenic fungi, oomycetes bacteria indicates specific determinants required activity. Strains varying amounts well metabolites, can influence spectrum activity, synergistic, additive, or negative manner. Further understanding overall variability these antimicrobials is essential evaluate short- long-term efficacy, therefore sustainability spp. biocontrol agents.

Language: Английский

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Isolation and Characterization of Bacillus Strains from Egyptian Mangroves: Exploring Their Endophytic Potential in Maize for Biological Control of Spodoptera frugiperda

Biology, Journal Year: 2024, Volume and Issue: 13(12), P. 1057 - 1057

Published: Dec. 17, 2024

The widespread use of pesticides to manage Spodoptera frugiperda has led significant challenges. This insect developed resistance 47 active insecticide ingredients. Therefore, endophytic entomopathogenic bacteria have been explored as an alternative pest management strategy, offering the potential reduce reliance on chemical pesticides. current study aims evaluate colonization indigenous marine Bacillus strains endophytes in maize plants and assess their insecticidal activity against S. frugiperda. Four inoculation methods—foliar application, seed treatment, soil drenching, a combination all three—were used establish plants. Our results showed that promising native exhibited both antibacterial effects neonates under laboratory conditions. Foliar application sp. Esh39 caused highest mortality rate (65%), followed by tequilensis R39 (60%). However, this method did not significantly enhance plant height or chlorophyll content. these warrants further investigation improve biological control via mediation. findings provide valuable insights into bacterial diversity functionality mangrove ecosystems pave way for innovative, sustainable strategies.

Language: Английский

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