Comparative gametogenesis and genomic signatures associated with pollen sterility in the seedless mutant of grapevine

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 3, 2025

Seedless grapes are in high demand for fresh and dry fruit consumption. Seedlessness (Vitis vinifera L.) is triggered by two different mechanisms: stenospermocarpy parthenocarpy. However, the key regulators of seed development their targets not well characterized. The present study used seeded grape hybrid ARI 516 its seedless mutant to understand molecular mechanisms controlling phenotype grapes. Gametogenesis studies demonstrated that exhibits pollen sterility due abnormal morphology, significantly low viability, a complete lack germination ability. macrogametophyte was smaller than 516. Transcriptomic comparisons were performed during three developmental stages, including pre-flowering stage E-L 15, anthesis 23, berry formation 31, altered processes Genes downregulated enriched male gametophyte development-related pathways, which may cause sterility. RNAseq results validated qRT-PCR. Genome sequence data also identify induced mutations mutant, revealed homozygous 25 heterozygous InDels genes related development. genome sequencing collectively indicate parthenocarpic aberrant physiological involved formation, maturation showed downregulation transcription factors target cell division, gibberellin biosynthesis signalling, wall development, organization, germination. This represents comprehensive attempt putative candidate associated with using genomic approaches.

Language: Английский

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Roles of sugar metabolism and transport in flower development

Current Opinion in Plant Biology, Journal Year: 2025, Volume and Issue: 85, P. 102722 - 102722

Published: April 6, 2025

Language: Английский

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Genetic effect of the Ph1 locus on transcriptome atlas of anther development‐related genes, meiotic chromosome behavior and agronomic traits in bread wheat

The Plant Journal, Journal Year: 2025, Volume and Issue: 122(3)

Published: May 1, 2025

SUMMARY Proper spatiotemporal expression of meiosis‐related genes (MRGs) and other male‐microsporogenesis/microgametogenesis‐related (MMRGs) is crucial for normal anther development, yet their patterns remain largely unknown in wheat. The Ph1 locus wheat known to contain the gene that plays a dual role promoting pairing between homologous chromosomes but repressing homoeologous chromosomes, its genetic function still unclear. Here, we investigated these issues by conducting comprehensive transcriptome analysis during development Chinese Spring (CS) ph1b deletion mutant under greenhouse field conditions. Our results revealed MRGs MMRGs are predominantly expressed pre‐meiosis stages, with also being highly meiotic‐II. Gene co‐expression showed C2H2 B3 transcriptional factors (TFs) associated MRGs, MYB regulators interacted mainly microgametogenesis. Deletion within failed induce compensatory activation counterparts, while outside environmental‐specific responses, especially meiotic‐II mature pollen stages. Notably, early disjunction bivalent primary factor leading defective meiocytes metaphase I. Furthermore, exhibited substantially delayed heading date, potentially contributing environment‐stable environment‐specific alterations fertility grain‐related traits. study highlights significant impact on previously unheeded effect meiotic chromosome agronomic traits, suggesting potential manipulations improvement.

Language: Английский

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Deciphering the Vulnerability of Pollen to Heat Stress for Securing Crop Yields in a Warming Climate

Plant Cell & Environment, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 25, 2024

ABSTRACT Climate change is leading to more frequent and severe extreme temperature events, negatively impacting agricultural productivity threatening global food security. Plant reproduction, the process fundamental crop yield, highly susceptible heatwaves, which disrupt pollen development ultimately affect seed‐set yields. Recent research has increasingly focused on understanding how grains from various crops react heat stress at molecular cellular levels. This surge in interest over last decade been driven by advances genomic technologies, such as single‐cell RNA sequencing, holds significant potential for revealing underlying regulatory reprogramming triggered throughout stages of development. review focuses affects gene networks, including response, unfolded protein autophagy, discusses impact these changes It highlights selection a key strategy improving tolerance leveraging genetic variability among grains. Additionally, genome‐wide association studies population screenings have shed light underpinnings traits major that respond high temperatures during male reproductive stages. Gene‐editing tools like CRISPR/Cas systems could facilitate precise modifications boost resilience. The information covered this valuable selecting employing approaches develop heat‐tolerant genotypes.

Language: Английский

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Callose Deficiency Modulates Plasmodesmata frequency and the intercellular space In Rice Anthers

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 11, 2024

Abstract Fertilization relies on pollen mother cells able to transit from mitosis-to-meiosis supply gametes. This process involves remarkable changes at the molecular, cellular and physiological levels including (but not limited to) remodelling of cell wall. During meiosis onset, cellulose content walls gradually declines with concurrent deposition polysaccharide callose in anther locules. We aim understand biological significance cellulose-to-callose turnover using electron microscopic analyses rice flowers. Our observations indicate that wild type anthers, transition coincides a gradual reduction number cytoplasmic connections called plasmodesmata. A mutant Oryza sativa synthase GSL5, impaired accumulation premeiotic meiotic displayed plasmodesmata frequency among tapetal suggesting role for maintenance. In addition, significant increase cell-cell distance between shaping was observed mutant. The results suggest during mitosis-meiosis is necessary maintain cell-to-cell optimal intercellular spacing locular explaining regulatory influence metabolism flowering plants. Highlights Cellulose-to-callose switch onset correlates plasmodesmata, meiocytes surrounding somatic cells. GSL5 (glucan like 5) affects but no other wall component (e.g., cellulose) except dyad stage. Impaired synthesis proposed alter frequency, extracellular transition. Increase negatively both wildtype

Language: Английский

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Callose Deficiency Modulates Plasmodesmata Frequency and Extracellular Distance in Rice Pollen Mother and Tapetal cells

Annals of Botany, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 14, 2024

Fertilization relies on pollen mother cells able to transit from mitosis meiosis supply gametes. This process involves remarkable changes at the molecular, cellular and physiological levels including (but not limited to) remodelling of cell wall. During onset, cellulose content walls gradually declines with concurrent deposition polysaccharide callose in anther locules. We aim understand biological significance cellulose-to-callose turnover using electron microscopic analyses rice flowers. Our observations indicate that wild type anthers, mitosis-to-meiosis transition coincides a gradual reduction number cytoplasmic connections called plasmodesmata. A mutant Oryza sativa synthase GSL5 (Osgsl5-3), impaired accumulation premeiotic meiotic displayed greater plasmodesmata frequency among tapetal suggesting role for maintenance. In addition, significant increase extracellular distance between shaping was observed Osgsl5-3 mutant. The results suggest callose-to-cellulose during mitosis-meiosis is necessary maintain cell-to-cell optimal central locular cells. Findings this study contribute our understanding regulatory influence metabolism initiation flowering plants.

Language: Английский

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In-Depth Understanding of Cytoplasmic Male Sterility by Metabolomics in Spring Stem Mustard (Brassica juncea var. tumida Tsen et Lee)

Horticulturae, Journal Year: 2024, Volume and Issue: 10(9), P. 896 - 896

Published: Aug. 24, 2024

Male sterility (MS) caused by aborted pollen is a vital germplasm resource. In this study, metabolomic, transcriptomic, and proteomic analyses were performed to investigate the molecular regulatory mechanism of cytoplasmic male (CMS) in flower buds sterile line 09-05A its maintainer line, 09-05B, Brassica juncea. Our metabolomic analysis revealed that 41 metabolites involved development fertility formation differentially accumulated between 09-05B at bi-nucleate stage B. Integrated omics indicated expression key genes proteins amino acid phenylpropanoid metabolic pathways was remarkably downregulated 09-05A. Furthermore, abnormal transcription factor (TF) related tapetum wall may affect CMS line. The results level critical metabolism; synthesis; TF genes, such as shikimate kinase, phenylalanine ammonia-lyase, MYB103, have led impaired development, thereby causing abortion This study provides new insights into mechanisms underlying juncea lays foundation for detailed studies on identity characteristics MS-related genes.

Language: Английский

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Floral Developmental Morphology and Biochemical Characteristics of Male Sterile Mutants of Lagerstroemia indica

Plants, Journal Year: 2024, Volume and Issue: 13(21), P. 3043 - 3043

Published: Oct. 30, 2024

Male sterility is a common phenomenon in higher plants and often plays an important role the selection of superior offspring. 'Xiang Yun' mutant

Language: Английский

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The establishment of the anther somatic niche with single cell sequencing

Developmental Biology, Journal Year: 2024, Volume and Issue: 518, P. 37 - 47

Published: Nov. 14, 2024

Language: Английский

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Transcription factor-dependent regulatory networks of sexual reproduction in Fusarium graminearum

mBio, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 26, 2024

ABSTRACT Transcription factors (TFs) involved in sexual reproduction filamentous fungi have been characterized. However, we little understanding of how these TFs synergize within regulatory networks resulting development. We investigated 13 Fusarium graminearum , whose knockouts exhibited abortive or arrested phenotypes during development to elucidate the transcriptional cascade underlying fruiting bodies. A Bayesian network was inferred based on transcriptomic data from key stages evaluated silico knockout impacts developmental among and guided transcriptomics experiments properly assess roles genes with same phenotypes. Additional transcriptome were collected for TF by stage at which their appeared cognate prediction. Global revealed that mating-type locus ( MAT genes) trigger a involving affected early Notably, PNA1 mutants produced exceptionally small protoperithecia, shown be an upstream activator several essential ascospore production. In addition, SUB1 excessive numbers wherein pheromone-related dysregulated expression. conclude play central suppressive initiating reproduction, respectively. This comprehensive investigation contributes our framework governing multicellular body plan F. . IMPORTANCE Understanding regulation is crucial elucidation complex reproductive biology performed gene transcription (TFs), demonstrating affecting distinct Using across development, robustness interactions using systems approach. discovered initiates cascade, identified as Conversely, found role, exhibiting protoperithecia due abnormally high expression genes. These findings highlight regulating other activity related contributing deeper mechanisms multiple regulate

Language: Английский

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