GmARF15 Enhances the Resistance of Soybean to Phytophthora sojae by Promoting GmPT10d Expression in Response to Salicylic Acid Signalling DOI Open Access
Yunlong Huo, Haiyuan Chen, Zhuo Zhang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 191 - 191

Published: Dec. 29, 2024

Phytophthora root and stem rot caused by sojae (P. sojae) is a globally prevalent oomycete disease. The use of resistant cultivars an effective environmentally friendly strategy to manage this It important understand the molecular mechanisms underlying response Glycine max (soybean) P. infection. In study, we demonstrated that isoflavonoid-specific prenyltransferase gene (GmPT10d, Glyma.10G070300) was significantly upregulated in soybean cultivar Williams 82 with high resistance Transgenic seedlings overexpressing GmPT10d exhibited enhanced sojae, those subjected RNA interference showed increased susceptibility pathogen. Yeast-one-hybrid electrophoretic mobility shift assays revealed GmARF15 could directly bind promoter GmPT10d. Further analysis function transgenic also sojae. Transactivation assay, luciferase qPCR promote expression indicated elevated salicylic acid levels were associated Taken together, these findings reveal regulatory mechanism which enhances potentially promoting through signaling pathway.

Language: Английский

CRISPR/Cas9-based mutant library screening: the discovery of novel genes regulating immune responses in cotton and rice DOI Creative Commons
Sang Ryeol Park, Seungmin Son

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 14, 2024

The environmental conditions play a crucial role in determining crop yield, which is essential for ensuring food and nutritional security. However, rapid climate change exacerbating global stress, leading to severe biotic pressures on crops. Therefore, enhancing resilience pathogens has become one of the most pressing challenges humanity. Large-scale mutant library screening efficient strategy identifying numerous genes associated with specific traits. revolutionary CRISPR/Cas9 system ushered new era construction library. its application plants been relatively scarce compared mammals, largely due accessibility. Fortunately, several research groups have recently developed CRISPR/Cas9-based libraries, successfully variety involved immunity. In this review, we present an overview discussion studies that generated significant results through use identify novel resistance stresses within field plant research.

Language: Английский

Citations

0

GmARF15 Enhances the Resistance of Soybean to Phytophthora sojae by Promoting GmPT10d Expression in Response to Salicylic Acid Signalling DOI Open Access
Yunlong Huo, Haiyuan Chen, Zhuo Zhang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 191 - 191

Published: Dec. 29, 2024

Phytophthora root and stem rot caused by sojae (P. sojae) is a globally prevalent oomycete disease. The use of resistant cultivars an effective environmentally friendly strategy to manage this It important understand the molecular mechanisms underlying response Glycine max (soybean) P. infection. In study, we demonstrated that isoflavonoid-specific prenyltransferase gene (GmPT10d, Glyma.10G070300) was significantly upregulated in soybean cultivar Williams 82 with high resistance Transgenic seedlings overexpressing GmPT10d exhibited enhanced sojae, those subjected RNA interference showed increased susceptibility pathogen. Yeast-one-hybrid electrophoretic mobility shift assays revealed GmARF15 could directly bind promoter GmPT10d. Further analysis function transgenic also sojae. Transactivation assay, luciferase qPCR promote expression indicated elevated salicylic acid levels were associated Taken together, these findings reveal regulatory mechanism which enhances potentially promoting through signaling pathway.

Language: Английский

Citations

0