Genome-wide identification and analysis of the TBL genes reveals NtTBL31 increases drought resistance of tobacco (Nicotiana tabacum)

Chemical and Biological Technologies in Agriculture, Journal Year: 2025, Volume and Issue: 12(1)

Published: March 22, 2025

Language: Английский

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Biocontrol efficacy of Bacillus velezensis YZU 173039 against Corynespora leaf spot on tobacco

Biocontrol Science and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 14

Published: March 24, 2025

Nicotiana tabacum possesses significant economic value and has been used medically recreationally due to its mildly irritating properties. In recent years, Corynespora cassiicola emerged as a major cause of yield losses in Chinese tobacco crops, yet effective control measures remain undeveloped. This present study characterised the morphological characteristics C. isolated from tobacco. For biocontrol purposes, strain (YZU 173039) exhibiting strong inhibitory effects on mycelium growth was identified, demonstrating an inhibition rate 66.0% vitro. identified Bacillus velezensis based features sequence analyses 16S rRNA gyrB gene. Not only did fermentation broth inhibit mycelial cassiicola, but volatile organic compounds (VOCs) also reduced melanin content downregulated expression genes associated with synthesis. Three scytalone dehydratase (SCD) family were predicted be potentially involved synthesis pathogenicity. Furthermore, found activate defence mechanisms tobacco, evidenced by changes plant defence-related enzymes (Phenylalanine Ammonia-lyase (PAL), peroxidase (POD), polyphenol oxidase (PPO)) levels three (NtAOS, NtMYC2a, NtJAZ1). Results greenhouse experiments indicated that could significantly reduce disease severity caused cassiicola. As promising agent, B. YZU 173039 potential occurrence spread leaf spot crops.

Language: Английский

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Isolation, Identification, and Biocontrol Mechanisms of Endophytic Burkholderia arboris DHR18 from Rubber Tree against Red Root Rot Disease

Microorganisms, Journal Year: 2024, Volume and Issue: 12(9), P. 1793 - 1793

Published: Aug. 29, 2024

Red root rot disease is a devastating fungal of rubber trees caused by Ganoderma pseudoferreum (Wakef). Biocontrols using beneficial microorganisms are safe and sustainable. We isolated DHR18 endophytic bacterium from healthy tree to obtain new efficient antagonistic for red affecting evaluated the mechanism action involved double culture assay, genome annotation analysis, ethyl acetate extraction method. The results revealed that strain inhibits G. growth has broad-spectrum antifungal activity secreting cell wall hydrolases (proteases chitinases), indole-3-acetic acid, siderophores. Furthermore, it fixes nitrogen in biofilm formation phosphate solubilisation, improving resistance growth. showed substances secreted mainly lipopeptides. Simultaneously, enhanced increasing activities defence enzymes superoxide dismutase, phenylalanine ammonia lyase, peroxidase, catalase, polyphenol oxidase. indicate B. arboris biocontrol potential could be used as candidate control trees.

Language: Английский

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Synergistic co-evolution of rhizosphere bacteria in response to acidification amelioration strategies: impacts on the alleviation of tobacco wilt and underlying mechanisms

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 1, 2024

Soil acidification represents a severe threat to tobacco cultivation regions in South China, exacerbating bacterial wilt caused by Ralstonia solanacearum . The comprehension of the underlying mechanisms that facilitate restoration rhizosphere microbial communities “healthy soils” is imperative for ecologically managing wilt. This study focuses on acidified soils have been subjected continuous 20 years. experimental treatments included lime (L), biochar (B), and combination (L+B), addition control group (CK). Utilizing biology niche theory, we assessed disease suppression effects, changes soil properties, co-evolution community. Each treatment significantly reduced 16.67% 20.14% compared (CK) ( p < 0.05) increased yield 7.86% 27.46% 0.05). (B) proved be most effective, followed lime-biochar (L+B). key factors controlling were identified through random forest regression analysis as an increase pH exchangeable bases, along with decrease acidity. However, alone led bulk density available nutrients, whereas both improved these parameters No significant correlation was found between abundance incidence. Nonetheless, all expanded ecological breadth average variation degree (AVD), enhanced positive interactions cohesion within community, intensified negative involving suggests optimizing community niches enhancing pathogen antagonism are mitigating soils. It recommends using mixtures amendments due their potential economic benefits. offers valuable insights strategies presents novel perspective research Solanaceous crops.

Language: Английский

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Cladophialophora guangxiense sp. nov., a New Species of Dark Septate Endophyte, Mitigates Tomato Bacterial Wilt and Growth Promotion Activities

Agronomy, Journal Year: 2024, Volume and Issue: 14(12), P. 2771 - 2771

Published: Nov. 22, 2024

Bacterial wilt of tomatoes, caused by Ralstonia solanacearum, is a significant soilborne disease that often causes reductions in the yield tomatoes. Dark septate endophytic fungi (DSE) represent potential biocontrol agents against plant pathogens can also enhance growth. To collect DSE with for biocontrol, fungus Cladophialophora guangxiense HX2 was isolated from rhizosphere soil sugarcane Hengzhou Guangxi Province, China, and novel species identified based on morphological properties DNA sequence analysis. C. demonstrated controlling effect 76.7% tomato bacterial promoted 0.5-fold increase seedling height. It colonized roots, enhancing activity antioxidant defensive enzyme systems. Transcriptomic qPCR approaches were used to study induction response strain infection comparing gene expression profiles. Gene ontology (GO) Kyoto Encyclopedia Genome (KEGG) pathway enrichment revealed tomatoes produce salicylic acid metabolism, ethylene-activated signaling, photosynthesis, phenylpropanoid biosynthesis infection. The IAA4 (3.5-fold change), ERF1 Hqt (1.5-fold change) substantially enhanced Hsc 70 (0.5-fold significantly reduced treatment group. This provides theoretical foundation further investigation into as biological agent prevention control wilt.

Language: Английский

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Biocontrol Potential of Endophytic Bacillus velezensis LSR7 Against Rubber Red Root Rot Disease

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(12), P. 849 - 849

Published: Dec. 9, 2024

To obtain an effective bacterial biocontrol strain against the fungal pathogen

Language: Английский

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