EIL (ethylene‐insensitive 3‐like) transcription factors in apple affect both ethylene‐ and cold response‐dependent fruit ripening

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(5)

Published: March 1, 2025

EIN3/EIL (ethylene-insensitive 3/EIN3-like) transcription factors are positive downstream transcriptional regulators of ethylene signalling. In apple (Malus × domestica), a small family MdEIL genes was identified, with four expressed in fruit. Transgenic lines were generated to manipulate MdEIL1 expression, and fruits sampled at harvest maturity after cold treatment. Their fruit ripening behaviour compared control contrasted ACC OXIDASE 1 antisense line (ACO1as) which produced no associated ethylene. Two transgenic showed strong co-suppression MdEIL1-4 expression as well reduced production, softening aroma while one overexpressing enhanced ripening. Key involved biosynthesis ethylene-dependent cell wall modification (MdXTH1, MdβGAL) (MdAFS1, MdoOMT1) downregulated the co-suppressed lines. Co-suppressed softening/volatile production treatment contrast ACO1as line, response-dependent (MdCBF2, dehydrins MdDHN2, -14, -16 MdNAC29a) remained cold-repressed. The action MdEILs shown using dual-luciferase reporter assays occur through direct activation MdAFS1, MdXTH1 MdβGAL promoters. Exogenous unable further stimulate promoter activation, but could. Promoter deletion analysis identified potential EIL binding sites MdAFS1 promoters electrophoretic mobility shift that MdEIL1-3 could all bind 32 bp fragment promoter. Together these results indicate contribute suite attributes via an manner, also response cold.

Language: Английский

---

Innovative composite edible coatings of chia seed gum, gum Arabic incorporated with Nano-emulsion of Oliveria decumbens essential oil for prolonging navel orange fruit shelf life

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142270 - 142270

Published: March 1, 2025

Language: Английский

---

Transcriptomic Analysis Reveals Dynamic Changes in Glutathione and Ascorbic Acid Content in Mango Pulp across Growth and Development Stages

Horticulturae, Journal Year: 2024, Volume and Issue: 10(7), P. 694 - 694

Published: July 1, 2024

Mango (Mangifera indica) is a highly valuable horticultural crop known for its quality and productivity. This study investigates the dynamic changes in physicochemical properties glutathione ascorbic acid metabolic pathways mango pulp across various growth development stages over two consecutive years (2021–2022 2022–2023) by transcriptomic analysis. Overall, results demonstrate that during different ripening periods, shows increased levels of total soluble solids, relative conductivity, glutathione, enzymes, while titratable acidity, malondialdehyde, reactive oxygen species, contents decreased. Moreover, analysis identified key differentially expressed genes from validated them with qRT-PCR. In comparisons, 1776, 2513, 828 DEGs were 30 vs. 60, 90, 60 90 days after flowering, respectively. Among them, seven primarily enriched relevant pathways, which included ascorbate peroxidase, oxidase, gamma-glutamyl transferase, transferases, glucose-6-phosphate dehydrogenase. The upregulation these indicates AsA respond well to scavenging species maintain normal functioning plants. research sheds light on molecular mechanisms pulp, providing insights into regulation antioxidant fruit development.

Language: Английский

---

Rubus idaeus RiACS1 Gene Is Involved in Ethylene Synthesis and Accelerates Fruit Ripening in Solanum lycopersicum

Agronomy, Journal Year: 2025, Volume and Issue: 15(1), P. 164 - 164

Published: Jan. 10, 2025

Raspberry is a berry whose fruit not tolerant to storage; breeding varieties with extended storage time and high comprehensive quality are significant for raspberries in cold regions. 1-Aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS) limiting enzyme the ethylene synthesis process, which plays essential roles ripening softening plants. In this study, RiACS1 gene raspberry (Rubus idaeus L.) variety ‘Polka’ was cloned. The overexpression vector constructed transformed into tomato plants using Agrobacterium tumefaciens infection method verify its function their reproductive development. gene, total length of 1476 bp, encoded protein 491 amino acids. subcellular localization analysis tobacco transient expression system revealed that RiACS1-GFP fusion mainly located nucleus. Compared control, flowering color turning transgenic strains were advanced, hardness reduced. Overexpression increased activity ACC synthase, release rate, respiration rate during transchromic phase. It changed substance content, content vitamin C anthocyanin decreased chlorophyll titrable at maturity stage. addition, relative levels synthesis-related genes such as SlACS4, SlACO3, SlACO1 while it SlACS2 These results suggested could promote early This study provided basis further modifying molecular biology techniques.

Language: Английский

---

Calcium carbide (CaC₂) ripening in fruits: Health risks, non‐destructive detection, quality control, and regulatory frameworks

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2025, Volume and Issue: 24(2)

Published: Feb. 19, 2025

Abstract The use of calcium carbide (CaC 2 ) for the artificial ripening fruits is a common practice in some regions, despite being banned many countries due to health concerns. CaC reacts with moisture produce acetylene gas, which accelerates process. However, presence gas and its byproducts ‐ripened can pose risks human health. Therefore, there need non‐destructive techniques detect ‐induced ensure safety quality fruits. This review explores scope application detection Various spectroscopic methods, including near‐infrared spectroscopy, Raman hyperspectral imaging, have been investigated their potential identify ripened using . Additionally, chemical tests sensor‐based approaches explored residues examines principles behind these discusses advantages, limitations, practical applications. Non‐destructive are promising detecting Spectroscopic methods allow rapid identification characteristic signatures fruits, whereas residues. These tools fruit authenticity, protecting consumer Further research needed improve sensitivity reliability wider food industry.

Language: Английский

---

ACS4 exerts a pivotal role in ethylene biosynthesis during the ripening of tomato fruits in comparison to ACS2

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(5)

Published: March 1, 2025

In the climacteric fruit tomato (Solanum lycopersicum), 1-aminocyclopropane-1-carboxylic acid (ACC) synthase 2 (ACS2) and ACS4 are jointly recognized as key enzymes in orchestrating System-2 ethylene biosynthesis during ripening. However, precise roles individual contributions of ACS2 within this process remain elusive. Here, we generate acs2, acs4 single knockout, acs2/4 double knockout mutants through CRISPR/Cas9 system. Our results reveal that leads to a modest decrease production, with minimal effects on contrast, unveils severe ripening defect akin observed mutant, which stems from profound disruption autocatalytic biosynthesis, ultimately resulting inadequate production vital for supporting Transcriptome analysis, conjunction exogenous treatment, conclusively demonstrates pronounced dose-dependent correlation between ethylene, wherein varying doses distinctly regulate expression substantial number ripening-related genes, eventually controlling both quality formation. These findings clarify pivotal role compared deepen our understanding fine-tuned regulation

Language: Английский

---

Revolutionizing Food Packaging with Biobased Polymers, Active and Intelligent Materials for Enhanced Food Safety and Sustainability: Review

Food and Bioprocess Technology, Journal Year: 2025, Volume and Issue: unknown

Published: May 8, 2025

Language: Английский

---

Ethylene Synergism Control Using Integrated Cold Plasma Technologies to Enhance Fruit and Vegetable Quality

Food Engineering Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 16, 2024

Language: Английский

---