Exogenous regulation of macronutrients promotes the accumulation of alkaloid yield in anisodus tanguticus (Maxim.) pascher DOI Creative Commons

Na Liu,

Chen Chen, Bo Wang

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: June 26, 2024

Abstract Background Anisodus tanguticus (Maxim.) Pascher ( A. ) is a valuable botanical for extracting tropane alkaloids, which are widely used in the pharmaceutical industry. Implementing appropriate cultivation methods can improve both quality and yield of . A two-year field experiment was conducted from 2021 to 2023 using single-factor randomized complete block design replicated three times. The study examined effects different nutrient levels (nitrogen: 0, 75, 150, 225, 300, 375 kg/ha; phosphorus: 600, 750, 900, 1050, 1200 potassium: 112.5, 187.5, 225 kg/ha) on growth, primary alkaloid contents, at growth stages (S-Greening, S-Growing, S-Wilting; T-Greening, T-Growing, T-Wilting) roots aboveground portions. Results Our results demonstrate that significantly affect accumulation High nitrogen (375 notably increased root biomass, while phosphorus had minimal effect, especially biomass. For content (scopolamine, anisodamine, anisodine, atropine), moderate level (225 most effective, followed by low potassium (75 kg/ha), with showing limited impact. Increased led decrease scopolamine content. During T-Growing period, addition yielded highest per unit area (205.79 kg/ha). In T-Wilting (750 resulted 146.91 kg/ha 142.18 kg/ha, respectively. This indicates has substantial effect accumulation, phosphorus. Douglas production function analysis suggests focusing biomass atropine maximize cultivation. Conclusions findings show optimum harvesting period optimal 75 600 or less.

Language: Английский

Identification and Molecular Mechanism of COX‐2 Inhibitors From Anisodus tanguticus: Ligand Fishing, In Vitro Validation, Molecular Docking, Molecular Dynamics, and ADMET Analysis DOI

Su Rong,

Yue Wang,

Nixia Tan

et al.

Biomedical Chromatography, Journal Year: 2025, Volume and Issue: 39(6)

Published: April 29, 2025

ABSTRACT Anisodus tanguticus (Maxim.) Pascher has demonstrated remarkable inhibitory effects on cyclooxygenase‐2 (COX‐2); however, the effective substances and molecular mechanism remain ambiguous. In this study, surface plasmon resonance (SPR) ligand fishing technology coupled with UPLC‐Q‐TOF‐MS analysis were applied to identify two COX‐2 binders, atropine fabiatrin, from A. extracts. vitro assays verified their potent effects, IC 50 values of 16.63 10.66 mM, respectively. To elucidate underlying we conducted docking dynamics simulations. Interaction revealed that both fabiatrin exhibit strong binding affinity structural stability COX‐2. Subsequent ADMET (absorption, distribution, metabolism, excretion, toxicity) predictions indicated had favorable pharmacokinetic properties low toxicity, suggesting potential as anti‐inflammatory agents. Notably, is first study demonstrate effect Overall, integrated approach developed here provides an efficient reliable strategy for identifying bioactive components complex traditional Chinese medicine (TCM) systems, which may offer a new perspective scientific basis research development naturally targeted drugs.

Language: Английский

Citations

0
Exogenous regulation of macronutrients promotes the accumulation of alkaloid yield in anisodus tanguticus (Maxim.) pascher DOI Creative Commons

Na Liu,

Chen Chen, Bo Wang

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: June 26, 2024

Abstract Background Anisodus tanguticus (Maxim.) Pascher ( A. ) is a valuable botanical for extracting tropane alkaloids, which are widely used in the pharmaceutical industry. Implementing appropriate cultivation methods can improve both quality and yield of . A two-year field experiment was conducted from 2021 to 2023 using single-factor randomized complete block design replicated three times. The study examined effects different nutrient levels (nitrogen: 0, 75, 150, 225, 300, 375 kg/ha; phosphorus: 600, 750, 900, 1050, 1200 potassium: 112.5, 187.5, 225 kg/ha) on growth, primary alkaloid contents, at growth stages (S-Greening, S-Growing, S-Wilting; T-Greening, T-Growing, T-Wilting) roots aboveground portions. Results Our results demonstrate that significantly affect accumulation High nitrogen (375 notably increased root biomass, while phosphorus had minimal effect, especially biomass. For content (scopolamine, anisodamine, anisodine, atropine), moderate level (225 most effective, followed by low potassium (75 kg/ha), with showing limited impact. Increased led decrease scopolamine content. During T-Growing period, addition yielded highest per unit area (205.79 kg/ha). In T-Wilting (750 resulted 146.91 kg/ha 142.18 kg/ha, respectively. This indicates has substantial effect accumulation, phosphorus. Douglas production function analysis suggests focusing biomass atropine maximize cultivation. Conclusions findings show optimum harvesting period optimal 75 600 or less.

Language: Английский

Citations

1