A Unique Expression Profile Responding to Powdery Mildew in Wild Emmer Wheat D430 DOI Open Access

Yintao Dai,

Ningning Yu,

Hongxing Xu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 242 - 242

Published: Dec. 30, 2024

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a disease that seriously harms wheat production and occurs in all wheat-producing areas around the world. Exploring Pm gene(s) developing resistant cultivars are preferred to control disease. Wild emmer (Triticum dicoccoides, 2n = 4x 28, AABB) has accumulated abundant gene resources for resistance powdery mildew during long process of natural evolution. In current study, WEW accession D430 was highly at whole-growth stage. Genetic analysis showed conferred single dominant locus or cross susceptible durum 647, tentatively named PmD430. Combining BSR-Seq analysis, molecular mapping, sequence alignment, PmD430 finally mapped Pm4 locus, identical Pm4b. Subsequently, 1871 DEGs between bulks were annotated analyzed Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment. Eight disease-related genes evaluated qRT-PCR exhibited unique expression pattern when invaded Bgt isolate E09 was, therefore, presented as latent targets regulating D430.

Language: Английский

A Unique Expression Profile Responding to Powdery Mildew in Wild Emmer Wheat D430 DOI Open Access

Yintao Dai,

Ningning Yu,

Hongxing Xu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 242 - 242

Published: Dec. 30, 2024

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a disease that seriously harms wheat production and occurs in all wheat-producing areas around the world. Exploring Pm gene(s) developing resistant cultivars are preferred to control disease. Wild emmer (Triticum dicoccoides, 2n = 4x 28, AABB) has accumulated abundant gene resources for resistance powdery mildew during long process of natural evolution. In current study, WEW accession D430 was highly at whole-growth stage. Genetic analysis showed conferred single dominant locus or cross susceptible durum 647, tentatively named PmD430. Combining BSR-Seq analysis, molecular mapping, sequence alignment, PmD430 finally mapped Pm4 locus, identical Pm4b. Subsequently, 1871 DEGs between bulks were annotated analyzed Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment. Eight disease-related genes evaluated qRT-PCR exhibited unique expression pattern when invaded Bgt isolate E09 was, therefore, presented as latent targets regulating D430.

Language: Английский

Citations

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