CRISPR/Cas9‐Mediated Genome Editing for Trait Improvement and Stress Tolerance in Leguminosae (Legume Family)

Plant Breeding, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 31, 2024

ABSTRACT Legumes represent a significant source of protein, roughage, minerals and carbohydrates in global diets. They are cultivated primarily for human consumption, forage as green manure. However, the growth productivity legumes often hindered by range biological environmental factors. The most prevalent diseases afflicting include downy mildew, fusarium root rot, southern blight common leaf spot. Additionally, stresses such salinity drought represents challenges. Over time, development cultivars with enhanced tolerance has emerged sustainable approach to combat impact these stresses. Although environmentally friendly, traditional breeding methods entail lengthy screening cross‐breeding protocols, which constrain their efficacy addressing climate challenges ensuring food security. Furthermore, approaches rely on natural availability genetic variation, may not always be exist specific traits, particularly context rapidly changing conditions. method is sufficiently precise, can result unintended introduction undesirable traits. intricacy presents an additional challenge making it difficult isolate enhance desired These impede capacity expeditiously develop that withstand emerging stresses, change. Genome editing powerful tool overcome limitations methods. By enabling precise targeted manipulation, genome traits or introduce new ones, thereby facilitating climate‐resilient agronomic varieties. In particular, clustered regularly interspaced short palindromic repeat/CRISPR associated protein (CRISPR/Cas9)‐mediated demonstrated considerable potential enhancing legume adaptability diverse stress conditions increasing crop yield. This study highlights advancements editing, focus CRISPR/Cas9 technology, means improve crops' resilience response pressures.

Language: Английский

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Developing microbial seed coating for enhancing seed vigour and prolonging storability in chickpea

South African Journal of Botany, Journal Year: 2024, Volume and Issue: 172, P. 289 - 301

Published: July 26, 2024

Language: Английский

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Demystifying the nutritional and anti-nutritional genetic divergence of Pakistani chickpea (Cicer arietinum L.) genetic resource via multivariate approaches

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: Oct. 4, 2024

Chickpeas are a highly versatile functional food legume that possesses the capacity to boost human health and has potential alleviate malnutrition-related deficiencies. To investigate whole seed-based nutritional anti-nutritional composition, set of 90 chickpea genotypes (66 desi 24 kabuli) was collected from different research organizations in Pakistan. Significant variation (Tukey HSD test, p < 0.05) perceived among for traits under investigation. The genotypes, with maximum total soluble proteins (TSPs) (34.92%), crude (CPs) (30.13%), reducing sugars (17.33 mg/g s. wt.), i.e., Punjab-2000 (desi); free amino acids (TFAs) (3.34 g/100 g DW), Wild Hybrid-15 (desi), albumins (227.67 Sheenghar-2000 globulins (720 ICCV-96030 salt-soluble (200 ILWC-247 (TSSs) (102.63 CM1051/11 non-reducing (95.28 NIAB-CH2016 starch content (83.69%), CH55/09 (kabuli); least value factors glutelin (3.33 Hybrid-9 hordein (1.38 Noor-2013 tannins (5,425 uM/g Hybrid-1 phytic acid (PA) (0.18 Bhakhar-2011 could be promising formulate health-promoting plant-based products. Data were also analyzed principal component analysis (PCA), correlation, agglomerative hierarchical clustering. PC-1 revealed highest contribution (20.83%) toward cumulative variability, positive factor loading delivered by TSSs (0.85) followed (0.729). Genotypes grouped into three distinct clusters based on high average values Cluster I encompassed mean CP content, albumins, hordein, glutelin; II TSPs, TSSs, sugars, globulins, starch, TFAs; III tannins, PA. Identified kabuli exhibiting superior seed quality minimal can used breeding programs aimed at improving future lines.

Language: Английский

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Traditional sweet flatbread of western India—Puran Poli

Discover Food, Journal Year: 2024, Volume and Issue: 4(1)

Published: Dec. 19, 2024

Language: Английский

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Unveilling heat-resilient chickpea (Cicer arietinum L.) lines under two mega chickpea growing regions in India using GGE biplot analysis

Indian Journal of Genetics and Plant Breeding (The), Journal Year: 2024, Volume and Issue: 84(04), P. 723 - 726

Published: Dec. 26, 2024

In the face of escalating uncertainties due to global climate change-induced heat stress, ensuring stability chickpea yields iscrucial for food security. To select stable and stress-tolerant genotypes, 25 advanced breeding lines, includingthree checks, were evaluated various phenological, yield, yield-related traits under diverse ecological field conditions. Undernormal sown conditions, IPC2021-71 (G13), ICC92944 (G21), IPC2019-170 (G14) showed greater desirability yield.Among these, outperformed in Kanpur, Punjab, Bhopal based on “which won where” criterion. Punjab Bhopalemerged as most informative locations GGE biplot “discrimination-and-representativeness” analysis. Under heatstress analysis revealed that IPC2021-165 (G6), IPC2015-52 (G9), IPC2011-61 (G10), (G21) stableperformers. Additionally, criterion highlighted G6 best performer New Delhi identified representative stress.

Language: Английский

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