Transcriptomic changes in donor soybean, dodder bridge, and the connected recipient soybean induced by cadmium addition

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: April 17, 2025

Cuscuta spp. (dodders) are parasitic plants that belong to the Convolvulaceae family. In nature, dodder often forms a bridge-like connection between two or more host like, which is known as bridge. Cadmium (Cd2+) an important heavy metal ion affects plant growth. However, it remains unclear whether Cd2+ treatment can directly indirectly induce transcriptomic changes in through this study, pot experiment was conducted investigate effects of on donor and neighboring recipient connected by Transcriptome analysis revealed significantly affected expression genes involved 'Plant-pathogen interaction', 'phenylpropanoid biosynthesis', 'isoflavonoid biosynthesis' pathways both at 2, 12, 24, 48 h. induced bridge, included processes related oxidation-reduction ('oxidation-reduction process', 'oxidoreductase activity', 'regulation transcription') Ca2+ signaling ('Plant-pathogen 'MAPK pathway', 'AMPK 'mTOR pathway'). Additionally, mRNA transfer observed from soybean dodder. mRNA, ROS might play crucial roles signal transduction process stress. could These results contribute better understanding how bridges respond environmental stresses.

Language: Английский

Metabolomic and Proteomic Analyses to Reveal the Role of Plant-Derived Smoke Solution on Wheat under Salt Stress

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8216 - 8216

Published: July 27, 2024

Salt stress is a serious problem, because it reduces the plant growth and seed yield of wheat. To investigate salt-tolerant mechanism wheat caused by plant-derived smoke (PDS) solution, metabolomic proteomic techniques were used. PDS which repairs inhibition under salt stress, contains metabolites related to flavonoid biosynthesis. Wheat was treated with solution proteins analyzed using gel-free/label-free technique. Oppositely changed associated protein metabolism signal transduction in biological processes, as well mitochondrion, endoplasmic reticulum/Golgi, plasma membrane cellular components compared control. Using immuno-blot analysis, results confirmed that ascorbate peroxidase increased decreased additional solution; however, H+-ATPase displayed opposite effects. Ubiquitin nevertheless, genomic DNA did not change. As part mitochondrion-related events, contents ATP recovered solution. These suggest enhances suppressed through regulation energy ubiquitin-proteasome system metabolism.

Language: Английский

Integrated Metabolomics and Transcriptomics Provide Key Molecular Insights into Floral Stage-Driven Flavonoid Pathway in Safflower

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 11903 - 11903

Published: Nov. 6, 2024

Safflower (

Language: Английский