Unraveling the Molecular Mechanisms of Blueberry Root Drought Tolerance Through Yeast Functional Screening and Metabolomic Profiling DOI Creative Commons
Xinyu Fan,

Beijia Lin,

Yahong Yin

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(24), P. 3528 - 3528

Published: Dec. 17, 2024

Blueberry plants are among the most important fruit-bearing shrubs, but they have shallow, hairless roots that not conducive to water and nutrient uptake, especially under drought conditions. Therefore, mechanism underlying blueberry root tolerance should be clarified. Hence, we established a yeast expression library comprising genes associated with responses stress. High-throughput sequencing technology enabled identification of 1475 potentially related tolerance. A subsequent KEGG enrichment analysis revealed 77 key six pathways: carbon energy metabolism, biosynthesis secondary metabolites, nucleotide amino acid genetic information processing, signal transduction, material transport catabolism. Metabolomic profiling drought-tolerant strains conditions detected 1749 differentially abundant metabolites (DAMs), including several up-regulated (organic acids, acids derivatives, alkaloids, phenylpropanoids). An integrative indicated encoding enzymes, GALM, PK, PGLS, PIP5K, modulate metabolism-related D-glucose 6-phosphate β-D-fructose 6-phosphate. Additionally, FDPS CCR were implicated in terpenoid phenylalanine biosynthesis, which affected metabolite contents (e.g., farnesylcysteine tyrosine). Furthermore, for GST GLT1, along eight DAMs, L-γ-glutamylcysteine L-ornithine, contributed while NDPK APRT linked purine thereby affecting certain inosine 3′,5′-cyclic GMP). Overall, functional screening system used this study effectively identified influencing tolerance, offering new insights into molecular mechanisms.

Language: Английский

Unraveling the Molecular Mechanisms of Blueberry Root Drought Tolerance Through Yeast Functional Screening and Metabolomic Profiling DOI Creative Commons
Xinyu Fan,

Beijia Lin,

Yahong Yin

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(24), P. 3528 - 3528

Published: Dec. 17, 2024

Blueberry plants are among the most important fruit-bearing shrubs, but they have shallow, hairless roots that not conducive to water and nutrient uptake, especially under drought conditions. Therefore, mechanism underlying blueberry root tolerance should be clarified. Hence, we established a yeast expression library comprising genes associated with responses stress. High-throughput sequencing technology enabled identification of 1475 potentially related tolerance. A subsequent KEGG enrichment analysis revealed 77 key six pathways: carbon energy metabolism, biosynthesis secondary metabolites, nucleotide amino acid genetic information processing, signal transduction, material transport catabolism. Metabolomic profiling drought-tolerant strains conditions detected 1749 differentially abundant metabolites (DAMs), including several up-regulated (organic acids, acids derivatives, alkaloids, phenylpropanoids). An integrative indicated encoding enzymes, GALM, PK, PGLS, PIP5K, modulate metabolism-related D-glucose 6-phosphate β-D-fructose 6-phosphate. Additionally, FDPS CCR were implicated in terpenoid phenylalanine biosynthesis, which affected metabolite contents (e.g., farnesylcysteine tyrosine). Furthermore, for GST GLT1, along eight DAMs, L-γ-glutamylcysteine L-ornithine, contributed while NDPK APRT linked purine thereby affecting certain inosine 3′,5′-cyclic GMP). Overall, functional screening system used this study effectively identified influencing tolerance, offering new insights into molecular mechanisms.

Language: Английский

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