Urinary biochemical ecology reveals microbiome-metabolite interactions and metabolic markers of recurrent urinary tract infection DOI
Michael L. Neugent, Neha V. Hulyalkar, Debasish Ghosh

et al.

Published: Oct. 24, 2024

Abstract Recurrent urinary tract infections (rUTIs) are a major clinical challenge in postmenopausal women and their increasing prevalence underscores the need to define interactions between host microbiome that may underlie rUTI susceptibility. A body of work has identified taxonomic profile female associate with aging, menopause, urinay disease. However, how this microbial community engages niche, including local biochemical environment urogenital tract, health disease is yet be fully defined. This study directly assesses differences urine, or ecology, associated recurrent infection (UTI) defines microbe-metabolite association network microbiome. By integrating metagenomic metabolomic data collected from controlled cohort rUTI, we find distinct metabolites, such as methionine sulfoxide (Met-SO) trimethylamine oxide (TMAO), diversity. We observe associations beta diversity unique metabolic networks uropathogenic Escherichia coli uroprotective Lactobacillus species, highlighting potential metabolite-driven ecological shifts influence UTI identify lipid signature active can accurately distinguish (AUC = 0.987) cases controls. Finally, using time-to-relapse deoxycholic acid (DCA) new prognostic indicator for recurrence. Together these findings suggest systemic processes susceptibility, opening avenues therapeutic intervention development more accurate diagnostic improve patient outcomes.

Language: Английский

In silico-based investigation of the molecular mechanism of Artocarpus communis seed hexane fraction against metabolic syndrome DOI
Amel Elbasyouni, Dhamodharan Prabhu,

Emmanuel Oluwatofunmi Akindoyin

et al.

Journal of Molecular Modeling, Journal Year: 2025, Volume and Issue: 31(2)

Published: Jan. 23, 2025

Language: Английский

Citations

0
Alterations in Methionine Cycle and Wnt/MAPK Signaling Associated with HMBi-Induced Cashmere Growth in Goats DOI Open Access

Minjie Xi,

Jiali Jiang, Wei Wang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1663 - 1663

Published: Feb. 15, 2025

Methionine (Met) was the first limiting amino acid identified in cashmere goats, and 2-hydroxy-4-(methylthio) butanoic isopropyl ester (HMBi) can effectively provide Met encourage growth goats. However, existing studies have primarily centered on trait of not delved into underlying molecular physiological mechanisms by which HMBi promotes In present study, we combined metabolomic transcriptomic approaches to reveal effects supplementation its impact gene expressions metabolic profiles within skin tissue Liaoning A total 14 female goats were randomly allocated control (CON) groups. The CON group received a basal diet, fed diet plus 1.27% HMBi. Our results show that significantly increased (p < 0.05) length decreased diameter metabolomics (variable importance projection >1 p concentrations Met, 2-Hydroxy-4-methylthiobutanoic (HMB), proline betaine, 10-hydroxydecanoic For HMB degradation cycle-related genes, compared with diets, diets elevated LDHD, MAT1A, AHCY 86.33%, 154.54%, 147.89% tissue, respectively. Regarding genes related cell proliferation differentiation, CCND1, CDK4, IVL, BMP4 113.31%, 107.93%, 291.33%, 186.21%, transcriptome evaluation differential expression mainly enriched Wnt MAPK signaling pathways. summary, these findings indicate cycle, Wnt, play important roles process HMBi, promoting

Language: Английский

Citations

0
Multiomics Analyses Demonstrate the Attenuation of Metabolic Cardiac Disorders Associated With Type 2 Diabetes by Stachydrine in Relation With the Transition of Gastrointestinal Microbiota DOI
Chaoxing Yang,

Huaping Zhong

Chemical Biology & Drug Design, Journal Year: 2025, Volume and Issue: 105(2)

Published: Feb. 1, 2025

Stachydrine (STA) has therapeutic effects on heart disorders. The current study assessed its Type 2 diabetes (T2D) induced cardiac disorders by focusing the heart-gut axis. Mice were subjected to high-fat diet (HFD) and streptozocin (STZ) induce such as inflammation structural deteriorations, which handled with STA. Changes regarding composition metabolism of gastrointestinal (GI) microbiota then determined using a multiomics strategy, including amplicon sequencing metabolomics. data showed that STA improved function, reduced intestinal permeability, suppressed in mice dose-dependent manner. However, compound had little influence overall alpha diversity gut microbiota, while it did beta diversity. analyses based strategy demonstrated certain GI microbial groups, Paramuribaculum, Allobaculum, Bifidobacterium, Adlercreutzia, responded administration, contributed alternatives metabolites gut. Correlation Duncaniella Ruminococcus negatively impacted health, Muribaculum, Prevotella positively influenced permeability health. Collectively, attenuated T2D-induced improving structure function suppressing inflammation, during homeostasis T2D changed an alternative state was different from healthy mice.

Language: Английский

Citations

0
Dynamic changes in volatile and non-volatile components of Jinggang honey pomelo (Citrus maxima (L.) Osbeck) during growth revealed through an untargeted metabolomics approach DOI
Yiyuan Duan, Zhongwei Liu, Qingqing Zhang

et al.

Food Chemistry, Journal Year: 2025, Volume and Issue: 478, P. 143654 - 143654

Published: March 1, 2025

Language: Английский

Citations

0
Unlocking hidden biomarkers: Exploring miswak (Salvadora persica L.) potential in tongue squamous cell carcinoma through UPLC-MS/MS and multivariate analysis DOI
Alaa A. El‐Banna, Reham S. Ibrahim,

Dina S. Ghallab

et al.

South African Journal of Botany, Journal Year: 2025, Volume and Issue: 180, P. 497 - 511

Published: March 30, 2025

Language: Английский

Citations

0
Phytoconstituents and Bioactivities of Cassia fistula Leaf and Stem Bark: Antioxidant Capacity and α-Glucosidase Inhibition via Molecular Docking and In Vitro Approaches DOI

Francis Xavier,

Muhammad Hanafi, Yesi Desmiaty

et al.

Biocatalysis and Agricultural Biotechnology, Journal Year: 2025, Volume and Issue: unknown, P. 103589 - 103589

Published: April 1, 2025

Language: Английский

Citations

0
Network Pharmacology Combined with Experimental Validation to Investigate the Mechanism of the Anti-Hyperuricemia Action of Portulaca oleracea Extract DOI Open Access

Yiming Zhang,

Shengying Zhu,

Yueming Gu

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(20), P. 3549 - 3549

Published: Oct. 19, 2024

Hyperuricemia (HUA) is a common metabolic disease caused by purine disorders in the body.

Language: Английский

Citations

1
Urinary biochemical ecology reveals microbiome-metabolite interactions and metabolic markers of recurrent urinary tract infection DOI
Michael L. Neugent, Neha V. Hulyalkar, Debasish Ghosh

et al.

Published: Oct. 24, 2024

Abstract Recurrent urinary tract infections (rUTIs) are a major clinical challenge in postmenopausal women and their increasing prevalence underscores the need to define interactions between host microbiome that may underlie rUTI susceptibility. A body of work has identified taxonomic profile female associate with aging, menopause, urinay disease. However, how this microbial community engages niche, including local biochemical environment urogenital tract, health disease is yet be fully defined. This study directly assesses differences urine, or ecology, associated recurrent infection (UTI) defines microbe-metabolite association network microbiome. By integrating metagenomic metabolomic data collected from controlled cohort rUTI, we find distinct metabolites, such as methionine sulfoxide (Met-SO) trimethylamine oxide (TMAO), diversity. We observe associations beta diversity unique metabolic networks uropathogenic Escherichia coli uroprotective Lactobacillus species, highlighting potential metabolite-driven ecological shifts influence UTI identify lipid signature active can accurately distinguish (AUC = 0.987) cases controls. Finally, using time-to-relapse deoxycholic acid (DCA) new prognostic indicator for recurrence. Together these findings suggest systemic processes susceptibility, opening avenues therapeutic intervention development more accurate diagnostic improve patient outcomes.

Language: Английский

Citations

0