Thidiazuron Enhances Strawberry Shoot Multiplication by Regulating Hormone Signal Transduction Pathways DOI Open Access
Fang Wang, Yali Li,

Yadan Pang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4060 - 4060

Published: April 25, 2025

Tissue culture-based rapid propagation is critical for genetic improvement and virus-free production of strawberries (Fragaria × ananassa). This study evaluated the optimal concentration thidiazuron (TDZ) shoot multiplication explored underlying molecular mechanisms. Strawberry explants were treated with TDZ at concentrations 0, 0.025, 0.05, 0.1, 0.4 mg·L−1 in vitro, growth, physiological changes, transcriptomic profiles analyzed after four weeks. The results identified 0.05 as most effective proliferation, yielding a significant increase leaf number. However, application inhibited plant height reduced chlorophyll, carotenoid, soluble sugar contents. Physiological analyses revealed that decreased endogenous cytokinin levels while elevating auxin concentrations. Transcriptomic analysis showed suppressed biosynthesis up-regulated oxidase expression, thereby modulating hormone homeostasis. Additionally, enhanced signaling pathway, which crucial cell division initiation, influenced auxin, gibberellin, brassinosteroid pathways to regulate differentiation. These findings suggest promotes strawberry primarily through signal transduction, providing insights optimizing tissue culture protocols.

Language: Английский

Enhancing Nutritional and Functional Properties of Broccoli Leaves Through Selenium Biofortification: Potential for Sustainable Agriculture and Bioactive Compound Valorization DOI Creative Commons

Melina Soledad Buttarelli,

Gabriel Céccoli, Betiana Soledad Trod

et al.

Agronomy, Journal Year: 2025, Volume and Issue: 15(2), P. 389 - 389

Published: Jan. 31, 2025

Selenium (Se) biofortification is a promising agronomic strategy to enhance the dietary intake of this essential micronutrient while simultaneously adding value agricultural by-products like Brassica oleracea L. var. italica leaves. This study evaluated effects foliar Se on fresh market broccoli cultivar (‘Belstar’) using selenite and selenate (1 2 mM). Growth performance, biochemical properties, nutraceutical quality, phytohormone profiles leaves were analyzed, highlighting their potential as functional by-products. Multivariate analysis revealed that mM application was most effective treatment, significantly improving several parameters. with increased nutrient content, including Se, Ca, S, Fe, Mn, Mg, Mo. It also enhanced soluble protein content (+2.2-fold), phenolic compounds (+1.5-fold), total antioxidant capacity (+1.4-fold) compared control plants. In sense, quality markedly improved, supporting use source bioactive ingredients. Additionally, assess practical applications, water-extracted Se-enriched demonstrated antifungal activity against plant pathogen Fusarium solani, attributed Se-induced alterations in profiles. These findings suggest Se-biofortified can serve sustainable nutrients for food industry. Furthermore, properties position them eco-friendly biopesticides combat pathogenic fungi, thereby promoting agriculture.

Language: Английский

Citations

1

Thidiazuron Enhances Strawberry Shoot Multiplication by Regulating Hormone Signal Transduction Pathways DOI Open Access
Fang Wang, Yali Li,

Yadan Pang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4060 - 4060

Published: April 25, 2025

Tissue culture-based rapid propagation is critical for genetic improvement and virus-free production of strawberries (Fragaria × ananassa). This study evaluated the optimal concentration thidiazuron (TDZ) shoot multiplication explored underlying molecular mechanisms. Strawberry explants were treated with TDZ at concentrations 0, 0.025, 0.05, 0.1, 0.4 mg·L−1 in vitro, growth, physiological changes, transcriptomic profiles analyzed after four weeks. The results identified 0.05 as most effective proliferation, yielding a significant increase leaf number. However, application inhibited plant height reduced chlorophyll, carotenoid, soluble sugar contents. Physiological analyses revealed that decreased endogenous cytokinin levels while elevating auxin concentrations. Transcriptomic analysis showed suppressed biosynthesis up-regulated oxidase expression, thereby modulating hormone homeostasis. Additionally, enhanced signaling pathway, which crucial cell division initiation, influenced auxin, gibberellin, brassinosteroid pathways to regulate differentiation. These findings suggest promotes strawberry primarily through signal transduction, providing insights optimizing tissue culture protocols.

Language: Английский

Citations

0