Propolis extract nanoparticles alleviate diabetes-induced reproductive dysfunction in male rats: antidiabetic, antioxidant, and steroidogenesis modulatory role DOI Creative Commons

Abram B. Emil,

Neven H. Hassan, Sally Ibrahim

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 23, 2024

Abstract Diabetes can affect male fertility via oxidative stress and endocrine system disruption. Nanomedicine based on natural products is employed to address diabetes complications. The current study aims investigate the potential beneficial effect of propolis extract nanoparticles against diabetes-induced testicular damage in rats. Sixty rats were randomly allocated six groups ( n = 10). first group served as a control group. second third received (Pr) (PrNPs). fourth diabetic that streptozotocin (STZ) (55 mg kg/bwt) single-dose i/p. fifth sixth are treated with Pr PrNPs. Both PrNPs at dose (100 mg/kg bwt) orally. After 60 days, animals euthanized, then pancreatic tissues collected for redox status evaluation, gene expression analysis, histopathological examination. Also, hormonal analysis (Insulin, total testosterone, luteinizing hormone (LH) ) along semen quality evaluation done. Results showed induction led deterioration showing reduction reduced glutathione (GSH) well elevation malondialdehyde (MDA), nitric oxide (NO) levels. relative transcript levels cytochrome P450 family 11 subfamily A member 1 (CYP11A1) , 3β-Hydroxysteroid dehydrogenase (HSD-3β) nuclear factor (erythroid-derived 2)-like 2 (NFE2L2) significantly down-regulated, While advanced glycation end-product receptor (AGER) was upregulated. Furthermore, disturbances observed. Upon treatment PrNPs, marked upregulation CYP11A1 HSD-3β NFE2L2 downregulation AGER, Hormones improved. In addition, enhanced. confirmed investigations. outperformed terms steroidogenesis pathway improvement, antioxidant defense mechanism augmentation, prospective antidiabetic activity.

Language: Английский

Propolis extract nanoparticles alleviate diabetes-induced reproductive dysfunction in male rats: antidiabetic, antioxidant, and steroidogenesis modulatory role DOI Creative Commons

Abram B. Emil,

Neven H. Hassan, Sally Ibrahim

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 23, 2024

Abstract Diabetes can affect male fertility via oxidative stress and endocrine system disruption. Nanomedicine based on natural products is employed to address diabetes complications. The current study aims investigate the potential beneficial effect of propolis extract nanoparticles against diabetes-induced testicular damage in rats. Sixty rats were randomly allocated six groups ( n = 10). first group served as a control group. second third received (Pr) (PrNPs). fourth diabetic that streptozotocin (STZ) (55 mg kg/bwt) single-dose i/p. fifth sixth are treated with Pr PrNPs. Both PrNPs at dose (100 mg/kg bwt) orally. After 60 days, animals euthanized, then pancreatic tissues collected for redox status evaluation, gene expression analysis, histopathological examination. Also, hormonal analysis (Insulin, total testosterone, luteinizing hormone (LH) ) along semen quality evaluation done. Results showed induction led deterioration showing reduction reduced glutathione (GSH) well elevation malondialdehyde (MDA), nitric oxide (NO) levels. relative transcript levels cytochrome P450 family 11 subfamily A member 1 (CYP11A1) , 3β-Hydroxysteroid dehydrogenase (HSD-3β) nuclear factor (erythroid-derived 2)-like 2 (NFE2L2) significantly down-regulated, While advanced glycation end-product receptor (AGER) was upregulated. Furthermore, disturbances observed. Upon treatment PrNPs, marked upregulation CYP11A1 HSD-3β NFE2L2 downregulation AGER, Hormones improved. In addition, enhanced. confirmed investigations. outperformed terms steroidogenesis pathway improvement, antioxidant defense mechanism augmentation, prospective antidiabetic activity.

Language: Английский

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