Comparative analysis of chloroplast genomes on Meliaceae species: insights into the evolution and species identification DOI Creative Commons
Zixuan Nie, Jikai Ma, Chengkun Wang

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 11, 2025

Introduction Meliaceae, a significant group in Sapindales, possesses material and medicinal value due to its applications timber bioactive compounds. However, the high morphological diversity of Meliaceae species lack comparative studies chloroplast (cp) genomes have led great challenges classification identification species. Methods In this study, we sequenced complete cp three Toona ( fargesii , ciliata sinensis ), conducted analyses these along with 29 previously published Meliaceae. Additionally, performed phylogenetic estimated divergence times Results The 32 ranged from 158,558 bp 160,978 length. Specifically, varied 159,242 159,617 These contained total 135 unique genes, comprising 90 protein-coding, 8 rRNA, 37 tRNA genes. Divergence time estimation revealed that family diverged into two subfamilies (Cedreloideae Melioideae) approximately 72.92 Ma (95% HPD: 60.62-87.01 Ma) Late Cretaceous. diversification Cedreloideae (47.86 Ma, 95% 47.42-48.22 occurred later than Melioideae (66.60 55.41-79.73 Ma). Furthermore, genomic analysis identified 52 116 simple sequence repeats (SSRs) twelve highly variable regions (HVRs) found among Discussion indicates most relatively recent origin, rapid occurring during Oligocene or Early Miocene epochs. Comparative exhibits relative conservation terms genome size, inverted-repeat (IR) boundary, structure, HVRs codon patterns. Although differences exist between subfamilies, overall similarity remains notably high. ycf 1, trn K- rps 16, ndh F- rpl exhibited highest nucleotide polymorphism within while 22 gene displayed genetic both subfamilies. As candidate molecular markers, may effectively distinguish findings not only provide insights evolution but also establish scientific foundation for future systematics

Language: Английский

Comparative analysis of chloroplast genomes on Meliaceae species: insights into the evolution and species identification DOI Creative Commons
Zixuan Nie, Jikai Ma, Chengkun Wang

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 11, 2025

Introduction Meliaceae, a significant group in Sapindales, possesses material and medicinal value due to its applications timber bioactive compounds. However, the high morphological diversity of Meliaceae species lack comparative studies chloroplast (cp) genomes have led great challenges classification identification species. Methods In this study, we sequenced complete cp three Toona ( fargesii , ciliata sinensis ), conducted analyses these along with 29 previously published Meliaceae. Additionally, performed phylogenetic estimated divergence times Results The 32 ranged from 158,558 bp 160,978 length. Specifically, varied 159,242 159,617 These contained total 135 unique genes, comprising 90 protein-coding, 8 rRNA, 37 tRNA genes. Divergence time estimation revealed that family diverged into two subfamilies (Cedreloideae Melioideae) approximately 72.92 Ma (95% HPD: 60.62-87.01 Ma) Late Cretaceous. diversification Cedreloideae (47.86 Ma, 95% 47.42-48.22 occurred later than Melioideae (66.60 55.41-79.73 Ma). Furthermore, genomic analysis identified 52 116 simple sequence repeats (SSRs) twelve highly variable regions (HVRs) found among Discussion indicates most relatively recent origin, rapid occurring during Oligocene or Early Miocene epochs. Comparative exhibits relative conservation terms genome size, inverted-repeat (IR) boundary, structure, HVRs codon patterns. Although differences exist between subfamilies, overall similarity remains notably high. ycf 1, trn K- rps 16, ndh F- rpl exhibited highest nucleotide polymorphism within while 22 gene displayed genetic both subfamilies. As candidate molecular markers, may effectively distinguish findings not only provide insights evolution but also establish scientific foundation for future systematics

Language: Английский

Citations

0