Development of an Assessment Method for Host Resistance of Alfalfa to Root Rot Caused by Rhizoctonia solani DOI

Muhammad Abdullah Akber,

S.S. Chu,

Xiangling Fang

et al.

Grass and Forage Science, Journal Year: 2025, Volume and Issue: 80(2)

Published: May 29, 2025

ABSTRACT Alfalfa ( Medicago sativa ) is the most important forage legume crop in China. Root rot disease caused by Rhizoctonia solani associated with severe yield and economic losses alfalfa. Using alfalfa varieties resistance considered effective sustainable way to manage However, there still a lack of methods evaluate host reactions R. . Ensuring consistent assessment through visual estimation often challenging when studying soil‐borne plant pathogen infections. This study firstly developed an method based on treatment inoculum density at 0.1%, 0.25%, 0.5% (w/w), inoculation millet seed‐based mycelial suspension inoculums, seedling ages two eight weeks. Nine were then used confirm method. Results showed that millet‐seed‐based method, combined density, resulted highest severity 78% roots 70% shoots, biomass reduction 86% 74% shoots. Seedlings inoculated weeks post‐germination exhibited higher susceptibility than those post‐germination. The selected age effectively differentiated varieties, Gannong 9 WL366 exhibiting strong , while Gongnong 1 Common susceptible. Besides, both harvesting time points (four after inoculation) levels among varieties. includes preparing inoculum, infecting two‐week‐old seedlings four or assessing growth parameters. provides for evaluating under controlled environmental conditions aim screen breed management root rot.

Language: Английский

Development of an Assessment Method for Host Resistance of Alfalfa to Root Rot Caused by Rhizoctonia solani DOI

Muhammad Abdullah Akber,

S.S. Chu,

Xiangling Fang

et al.

Grass and Forage Science, Journal Year: 2025, Volume and Issue: 80(2)

Published: May 29, 2025

ABSTRACT Alfalfa ( Medicago sativa ) is the most important forage legume crop in China. Root rot disease caused by Rhizoctonia solani associated with severe yield and economic losses alfalfa. Using alfalfa varieties resistance considered effective sustainable way to manage However, there still a lack of methods evaluate host reactions R. . Ensuring consistent assessment through visual estimation often challenging when studying soil‐borne plant pathogen infections. This study firstly developed an method based on treatment inoculum density at 0.1%, 0.25%, 0.5% (w/w), inoculation millet seed‐based mycelial suspension inoculums, seedling ages two eight weeks. Nine were then used confirm method. Results showed that millet‐seed‐based method, combined density, resulted highest severity 78% roots 70% shoots, biomass reduction 86% 74% shoots. Seedlings inoculated weeks post‐germination exhibited higher susceptibility than those post‐germination. The selected age effectively differentiated varieties, Gannong 9 WL366 exhibiting strong , while Gongnong 1 Common susceptible. Besides, both harvesting time points (four after inoculation) levels among varieties. includes preparing inoculum, infecting two‐week‐old seedlings four or assessing growth parameters. provides for evaluating under controlled environmental conditions aim screen breed management root rot.

Language: Английский

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