De novo assembly and comparative analysis of the first complete mitogenome in Distylium (Distylium racemosum) DOI Creative Commons

Yaling Wang,

Zhongxiao Zhang,

Xinru Chen

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: May 15, 2025

The genus Distylium (Hamamelidaceae) is highly valued for its applications in ornamental horticulture, industry, and construction. Although plastid genomes (plastomes) of multiple species have been characterized, no mitochondrial (mitogenomes) reported this genus. In study, we assembled annotated the complete mitogenome racemosum using HiFi sequencing data. comprises a longer circular chromosome shorter linear (904,264 bp total length), revealing structurally complex conformation. We 67 genes, including 43 protein-coding genes (PCGs), 21 tRNA three rRNA genes. Analyses identified exceptionally high repetitive sequence content, with 304 simple repeats, 1,508 dispersed 50 tandem representing highest repeat content among Saxifragales mitogenomes to date. Additionally, 49 DNA sequences were detected, only one plastid-derived gene ( trnC-GCA ) transferred mitogenome. predicted 697 RNA editing sites across 42 PCGs, further underscoring genome’s dynamic evolution. Phylogenetic reconstruction based on plastomes from 18 indicated D. occupied basal position within Saxifragales, which consistent APG IV classification system. This study provides first comprehensive mitogenomic resource genus, offering valuable insights molecular classification, identification, germplasm conservation plants.

Language: Английский

De novo assembly and comparative analysis of the first complete mitogenome in Distylium (Distylium racemosum) DOI Creative Commons

Yaling Wang,

Zhongxiao Zhang,

Xinru Chen

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: May 15, 2025

The genus Distylium (Hamamelidaceae) is highly valued for its applications in ornamental horticulture, industry, and construction. Although plastid genomes (plastomes) of multiple species have been characterized, no mitochondrial (mitogenomes) reported this genus. In study, we assembled annotated the complete mitogenome racemosum using HiFi sequencing data. comprises a longer circular chromosome shorter linear (904,264 bp total length), revealing structurally complex conformation. We 67 genes, including 43 protein-coding genes (PCGs), 21 tRNA three rRNA genes. Analyses identified exceptionally high repetitive sequence content, with 304 simple repeats, 1,508 dispersed 50 tandem representing highest repeat content among Saxifragales mitogenomes to date. Additionally, 49 DNA sequences were detected, only one plastid-derived gene ( trnC-GCA ) transferred mitogenome. predicted 697 RNA editing sites across 42 PCGs, further underscoring genome’s dynamic evolution. Phylogenetic reconstruction based on plastomes from 18 indicated D. occupied basal position within Saxifragales, which consistent APG IV classification system. This study provides first comprehensive mitogenomic resource genus, offering valuable insights molecular classification, identification, germplasm conservation plants.

Language: Английский

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