Rodent models of post-traumatic stress disorder: behavioral assessment

Translational Psychiatry, Journal Year: 2020, Volume and Issue: 10(1)

Published: May 6, 2020

Although the etiology and expression of psychiatric disorders are complex, mammals show biologically preserved behavioral neurobiological responses to valent stimuli which underlie use rodent models post-traumatic stress disorder (PTSD). PTSD is a complex phenotype that difficult model in rodents because it diagnosed by patient interview influenced both environmental genetic factors. However, given results from traumatic experiences, can simulate induction development. By manipulating type, intensity, duration, frequency, preclinical reflect core phenotypes, measured through various assays. Paradigms precipitate applying physical, social, psychological stressors individually or combination. This review discusses methods used trigger evaluate PTSD-like phenotypes. It highlights studies employing each evaluates their translational efficacies against DSM-5, validity criteria, criteria proposed Yehuda Antelman's commentary 1993. intended aid paradigm selection informing readers about models, benefits clinical community, challenges associated with opportunities for future work. To inform relevance human psychopathology, we propose incorporate test batteries, individual differences, sex strain stock early life effects, biomarkers, stringent success drug development, Research Domain Criteria, technological advances, cross-species comparisons. We conclude that, despite challenges, animal will be pivotal advances understanding neurobiology stress.

Language: Английский

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Ginsenoside Rg1 Prevents PTSD-Like Behaviors in Mice Through Promoting Synaptic Proteins, Reducing Kir4.1 and TNF-α in the Hippocampus

Molecular Neurobiology, Journal Year: 2020, Volume and Issue: 58(4), P. 1550 - 1563

Published: Nov. 19, 2020

Language: Английский

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The advent of fear conditioning as an animal model of post-traumatic stress disorder: Learning from the past to shape the future of PTSD research

Neuron, Journal Year: 2021, Volume and Issue: 109(15), P. 2380 - 2397

Published: June 18, 2021

Language: Английский

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Mesenchymal Stem Cell-Derived Exosomes and Intervertebral Disc Regeneration: Review

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(13), P. 7306 - 7306

Published: June 30, 2022

Intervertebral disc degeneration (IVDD) is a common cause of lower back pain (LBP), which burdens individuals and society as whole. IVDD occurs result aging, mechanical trauma, lifestyle factors, certain genetic abnormalities, leads to loss nucleus pulposus, alteration in the composition extracellular matrix, excessive oxidative stress, inflammation intervertebral disc. Pharmacological surgical interventions are considered boon for treatment IVDD, but effectiveness those strategies limited. Mesenchymal stem cells (MSCs) have recently emerged possible promising regenerative therapy due their paracrine effect, restoration degenerated cells, capacity differentiation into cells. Recent investigations shown that pleiotropic effect MSCs not related mediated by secretion soluble factors. Early studies demonstrated MSC-derived exosomes therapeutic potential treating promoting cell proliferation, tissue regeneration, modulation inflammatory response, reduced apoptosis. This paper highlights current state field with further future developments, applications, challenges.

Language: Английский

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Chronic stress and endothelial dysfunction: mechanisms, experimental challenges, and the way ahead

AJP Heart and Circulatory Physiology, Journal Year: 2020, Volume and Issue: 319(2), P. H488 - H506

Published: July 3, 2020

Although chronic stress is an important risk factor for cardiovascular diseases (CVD) onset, the underlying mechanisms driving such pathophysiological complications remain relatively unknown. Here, dysregulation of innate response systems and effects downstream mediators are strongly implicated, with vascular endothelium emerging as a primary target excessive glucocorticoid catecholamine action. Therefore, this review article explores development stress-related endothelial dysfunction by focusing on following: 1) assessing phenomenon complexities surrounding notion, 2) discussing mechanistic links between dysfunction, 3) evaluating utility various preclinical models currently employed to study onset stress-mediated dysfunction. The data reveal that play role in our efforts gain increased understanding It provides good foundation going forward, we propose further should be made more clearly define concept standardize protocols animal specific guidelines better indicate mental simulated.

Language: Английский

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A novel arousal-based individual screening reveals susceptibility and resilience to PTSD-like phenotypes in mice

Neurobiology of Stress, Journal Year: 2020, Volume and Issue: 14, P. 100286 - 100286

Published: Dec. 24, 2020

Translational animal models for studying post-traumatic stress disorder (PTSD) are valuable elucidating the poorly understood neurobiology of this neuropsychiatric disorder. These should encompass crucial features, including persistence PTSD-like phenotypes triggered after exposure to a single traumatic event, trauma susceptibility/resilience and predictive validity. Here we propose novel arousal-based individual screening (AIS) model that recapitulates all these features. The AIS was designed by coupling traumatization (24 h restraint) C57BL/6 J mice with screening. This consists z-normalization post-trauma changes in startle reactivity, which is measure arousal depending on neural circuits conserved across mammals. Through model, identified susceptible showing long-lasting hyperarousal (up 56 days post-trauma), resilient normal arousal. Susceptible further showed persistent exaggerated fear reactivity avoidance trauma-related cue 75 increased avoidance-like behavior social/cognitive impairment. Conversely, adopted active coping strategies, behaving like control mice. We uncovered transcriptional signatures driven PTSD-related genes as well dysfunction hypothalamic–pituitary–adrenal axis, corroborated segregation susceptible/resilient subpopulations obtained through correlated susceptibility/resilience. Impaired hippocampal synaptic plasticity also observed Finally, chronic treatment paroxetine ameliorated findings indicate might be new translational study features PTSD. It shed light unclear PTSD identify pharmacological targets difficult-to-treat

Language: Английский

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Phosphodiesterase inhibitors in psychiatric disorders

Psychopharmacology, Journal Year: 2023, Volume and Issue: 240(6), P. 1201 - 1219

Published: April 15, 2023

Language: Английский

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Geraniol attenuates behavioral and neurochemical impairments by inhibitions of HPA-axis and oxido-inflammatory perturbations in mice exposed to post-traumatic stress disorder

Journal of Psychiatric Research, Journal Year: 2023, Volume and Issue: 168, P. 165 - 175

Published: Oct. 27, 2023

Language: Английский

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Post-traumatic Stress Disorder: Focus on Neuroinflammation

Molecular Neurobiology, Journal Year: 2023, Volume and Issue: 60(7), P. 3963 - 3978

Published: April 1, 2023

Language: Английский

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Microbial composition, functionality, and stress resilience or susceptibility: unraveling sex-specific patterns

Biology of Sex Differences, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 26, 2024

Abstract Background Following exposure to traumatic stress, women are twice as likely men develop mood disorders. Yet, individual responses such stress vary, with some people developing stress-induced psychopathologies while others exhibit resilience. The factors influencing sex-related disparities in affective disorders well variations resilience remain unclear; however, emerging evidence suggests differences the gut microbiota play a role. In this study, using single prolonged (SPS) model of post-traumatic disorder, we investigated pre- and post-existing microbial composition, functionality, metabolites that affect susceptibility or each sex. Methods Male female Sprague–Dawley rats were randomly assigned control SPS groups. Two weeks following SPS, animals exposed battery behavioral tests decapitated day later. Based on their anxiety index, they further categorized SPS-resilient (SPS-R) SPS-susceptible (SPS-S). On dissection, cecum, selected brain tissues isolated. Stool samples collected before after whereas urine taken 30 min into SPS. Results Before exposure, sympathoadrenal axis exhibited alterations within male subgroups only. Expression tight junction protein claudin-5 was lower SPS-S males, but higher SPS-R females Across alpha diversity remained consistently males compared females. Beta revealed distinct separations between susceptible groups separation becoming evident resilient At genus level, Lactobacillus, Lachnospiraceae_Incertae_Sedis, Barnesiella sex-specific alterations, displaying opposing abundances Additionally, changes observed predictive functionality targeted functional modules both Alterations short-chain fatty acids (SCFAs), also observed, major minor SCFAs being branched-chain Conclusion This study highlights post-trauma metabolites, associated rats. findings underscore importance therapeutic strategies effectively address stress-related Highlights induces divergent social rodents. displayed less anxiety-like behavior initiated more interactions towards juvenile rat than males. Sex-specific pre-existing SPS-induced composition elevated cecal acetate levels, heightened SCFAs.

Language: Английский

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