Lineage-specific neutralising antibodies after SARS-CoV-2 mild disease. Immune boosting effect of vaccination DOI Creative Commons
Carlos Daviña‐Núñez, Sonia Pérez‐Castro, Jorge Julio Cabrera

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

Abstract We followed a group of 105 non-vaccinated individuals after Alpha or Delta SARS-CoV-2 mild disease, measuring the viral shedding (qRT-PCR, dPCR and subgenomic RNA-E) humoral response (commercial immunoassay pseudovirus live virus neutralisation) up to six months. Sixty nine patients received vaccination boost during follow-up period (n=95). Subgenomic RNA-E showed shorter until negativity (mean 2.2 weeks) compared gRNA 5.2 weeks). A high correlation between qRT-PCR was found for load estimation, even when no nucleic acid extraction used in (R 2 = 0.87). Post-convalescent sera strongest neutralisation against variant natural exposure, while capacity Omicron variants significantly lower other variants. Additionally, results suggested that commercial immunoassays may not accurately predict protection different than exposure. An immune boosting effect evident. Variant-specific neutralising antibodies were detected one month infection. Although short lived, maximum igG observed hybrid immunisation (natural infection + vaccination). This study also points potential improvements clinical management cases. Firstly, is potentially more accurate biomarker infectivity current qRT-PCRs using genomic RNA as target. Secondly, accuracy high-throughput must be validated order estimate specific organise campaigns. Our findings could play role implementation vaccine programs. Author Summary Years related infections challenged healthcare systems whole world, optimal strategy deal with diagnosis, quarantines patterns still matter debate. The interplay immunity circulating complex, qPCR diagnosis extend quarantines, detection does necessarily mean remains infectious. studied infected pre-Omicron how their variant-specific reacted past present SARS-CoV-2. In both Delta, neutralise own better came before after. Perhaps most importantly, lowest dynamics patients, testing PCR techniques targets. detectable nasopharyngeal samples time it has been good marker infectivity. Using sgRNA instead target reduce hospital bed occupation quarantine time. Overall, we hope these help guide pandemic diagnostic control future.

Language: Английский

Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis DOI Creative Commons
Laura Sancha Domínguez,

Ana Cotos Suárez,

María Sánchez Ledesma

et al.

Diagnostics, Journal Year: 2024, Volume and Issue: 14(9), P. 931 - 931

Published: April 29, 2024

Infectious diseases account for about 3 million deaths per year. The advent of molecular techniques has led to an enormous improvement in their diagnosis, both terms sensitivity and specificity the speed with which a clinically useful result can be obtained. Digital PCR, or 3rd generation is based on series technical modifications that more sensitive techniques, resistant action inhibitors capable direct quantification without need standard curves. This review presents main applications have been developed diagnosis viral, bacterial, parasitic infections potential prospects clinical use this technology.

Language: Английский

Citations

7
Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis DOI Open Access
Laura Sancha Domínguez,

Ana Cotos Suárez,

María Sánchez Ledesma

et al.

Published: March 20, 2024

A Infectious diseases account for about 3 million deaths per year. The advent of molecular techniques has led to an enormous improvement in their diagnosis, both terms sensitivity and specificity the speed with which a clinically useful result can be obtained. Digital PCR, or 3rd generation is based on series technical modifications that more sensitive techniques, resistant action inhibitors capable direct quantification, without need standard curves.This review presents main applications have been developed diagnosis viral, bacterial parasitic infections, potential prospects clinical use this technology.

Language: Английский

Citations

4
Subgenomic RNA and Limited Cross-Reactive Neutralising Antibodies Point to Potential Improvements in SARS-CoV-2 Clinical Handling DOI Open Access
Carlos Daviña‐Núñez, Sonia Pérez‐Castro, Jorge Julio Cabrera

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 2948 - 2948

Published: March 24, 2025

The current clinical management of SARS-CoV-2 disease control and immunity may be not optimal anymore. Reverse transcription polymerase chain reaction (RT-PCR) genomic viral RNA is broadly used for diagnosis, even though the virus still detectable when it already non-infectious. Regarding serology, commercial assays mostly rely on ancestral spike detection despite significant changes in genetic sequence circulating variants. We followed a group 105 non-vaccinated individuals, measuring their shedding until negativity antibody response up to six months. mean period negative RT-PCR result was 2.2 weeks using subgenomic RNA-E as target, 5.2 target. Our neutralising results suggest that, challenged against variant different from first exposure, immunoassays are suboptimal at predicting capacity sera. Additionally, anti-Alpha anti-Delta antibodies showed very low cross-reactivity between This study provides insights into immune pre-Omicron variants like Alpha Delta, which have been understudied published literature. These conclusions point potential improvements cases order organise vaccination campaigns select monoclonal treatments.

Language: Английский

Citations

0
The potential application of digital PCR in Detecting Different SARS-CoV-2 Viral Loads DOI
Chunyan Chang, Lingling Li,

Yating Guo

et al.

Journal of Virological Methods, Journal Year: 2025, Volume and Issue: unknown, P. 115151 - 115151

Published: April 1, 2025

Language: Английский

Citations

0
Viruses in Wastewater—A Concern for Public Health and the Environment DOI Creative Commons
Coralia Bleoţu, Lilia Matei, Laura Denisa Dragu

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(7), P. 1430 - 1430

Published: July 14, 2024

Wastewater monitoring provides essential information about water quality and the degree of contamination. Monitoring these waters helps identify manage risks to public health, prevent spread disease, protect environment. Standardizing appropriate most accurate methods for isolation identification viruses in wastewater is necessary. This review aims present major classes wastewater, as well concentration, isolation, assess health implement corrective measures control viral infections. Last but not least, we propose evaluate current strategies treatment new alternative disinfection.

Language: Английский

Citations

2
Lineage-specific neutralising antibodies after SARS-CoV-2 mild disease. Immune boosting effect of vaccination DOI Creative Commons
Carlos Daviña‐Núñez, Sonia Pérez‐Castro, Jorge Julio Cabrera

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

Abstract We followed a group of 105 non-vaccinated individuals after Alpha or Delta SARS-CoV-2 mild disease, measuring the viral shedding (qRT-PCR, dPCR and subgenomic RNA-E) humoral response (commercial immunoassay pseudovirus live virus neutralisation) up to six months. Sixty nine patients received vaccination boost during follow-up period (n=95). Subgenomic RNA-E showed shorter until negativity (mean 2.2 weeks) compared gRNA 5.2 weeks). A high correlation between qRT-PCR was found for load estimation, even when no nucleic acid extraction used in (R 2 = 0.87). Post-convalescent sera strongest neutralisation against variant natural exposure, while capacity Omicron variants significantly lower other variants. Additionally, results suggested that commercial immunoassays may not accurately predict protection different than exposure. An immune boosting effect evident. Variant-specific neutralising antibodies were detected one month infection. Although short lived, maximum igG observed hybrid immunisation (natural infection + vaccination). This study also points potential improvements clinical management cases. Firstly, is potentially more accurate biomarker infectivity current qRT-PCRs using genomic RNA as target. Secondly, accuracy high-throughput must be validated order estimate specific organise campaigns. Our findings could play role implementation vaccine programs. Author Summary Years related infections challenged healthcare systems whole world, optimal strategy deal with diagnosis, quarantines patterns still matter debate. The interplay immunity circulating complex, qPCR diagnosis extend quarantines, detection does necessarily mean remains infectious. studied infected pre-Omicron how their variant-specific reacted past present SARS-CoV-2. In both Delta, neutralise own better came before after. Perhaps most importantly, lowest dynamics patients, testing PCR techniques targets. detectable nasopharyngeal samples time it has been good marker infectivity. Using sgRNA instead target reduce hospital bed occupation quarantine time. Overall, we hope these help guide pandemic diagnostic control future.

Language: Английский

Citations

0