Public health concerns of multifaceted exposures to metal and metalloid mixtures: a systematic review

Environmental Monitoring and Assessment, Journal Year: 2025, Volume and Issue: 197(5)

Published: April 2, 2025

Language: Английский

Investigating Bergamot Essential Oil (BEO) Properties: Cytoprotection in Neuronal Cells Exposed to Heavy Metals and Antibacterial Activities

Antioxidants, Journal Year: 2025, Volume and Issue: 14(4), P. 400 - 400

Published: March 27, 2025

Bergamot [Citrus × limon (L.) Osbeck, syn. C. bergamia (Risso) Risso & Poit.] is primarily cultivated in the Calabria region of Italy and exploited food perfumery industry. The epicarp its fruit a rich source essential oil (BEO) containing mainly monoterpenes, which are known for their diverse biological activities, including antimicrobial, anti-inflammatory, antiproliferative, neuromodulatory effects. Emerging evidence suggests that oxidative stress plays central role pathogenesis neurodegenerative diseases, particularly Alzheimer’s disease (AD), where it contributes to neuronal dysfunction cell death. Moreover, heavy metal exposure has been identified as key environmental factor exacerbating neurodegeneration AD. This study aimed explore whether BEO could mitigate (Cd2+, Hg2+, Pb2+)-induced neurotoxicity SH-SY5Y cells, model system brain cells. MTT calcein-AM assays were performed examine viability cells after each itself, or combination with BEO, whereas LDH assay was carried out determine effects towards necrotic death induced by metals. Furthermore, DCFH-DA protect from metal-induced stress. also investigated antibacterial properties on different Gram-positive Gram-negative bacterial strains belonging ATCC collection. These results suggest may help counteract damage, Cd2+ toxicity, potentially reducing one risk factors associated Additionally, antimicrobial reinforce relevance preventing infections contribute neuroinflammation

Language: Английский

Comparative Mutational Analysis and the Glycosylation Patterns of a Peruvian Isolated Avian Influenza A Virus H5N1: Exploring Possible Viral Spillover Events Within One Health Approach

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(4), P. 392 - 392

Published: April 21, 2025

(1) Background: The ongoing panzootic of highly pathogenic avian influenza virus (HPAIV) subtype H5N1, clade 2.3.4.4b, has decimated wild/domestic birds and mammals’ populations worldwide with reports sporadic cases in humans. (2) Methods: This study aimed to compare the mutational profile H5N1 Influenza isolated from a Peruvian natural reserve, recent data other related international studies made human different species domestic wild mammals. Briefly, near complete protein sequences coming Calidris alba were analyzed at multisegmented level, together 55 samples collected between 2022 2024 countries. Moreover, glycosylation patterns also predicted silico. (3) Results: A total 603 amino acid changes found among viruses analyzed, underscoring detection critical mutations HA:11I, HA:211I, HA:336T, HA:492D, HA:527I, NA:10T, NA:269L, NA:405T, NP:377N, PA:57R, PA:68S, PA:322V/L, PA:432I, PB2:539V, PB1:207R, PB1:375N, PB1:264D, PB1:429R, PA-X:250Q, PB1-F2:65R, PB1-F2:42Y, as well PA:13V, PA-X:13V, PA20T, PA-X:20T, PA:36T PA-X:36T, PA:45S, PA-X:45S, PA:57Q, PA-X:57Q, PA:61I, PA-X:61I, PA-X:68S, PA:70V, PA-X:70V, PA:75Q, PA-X:75Q, PA:85T, PA-X:85T, PA:86I, PA-X:86I, PA:100I, PA-X:100I, PA:142E, PA-X:142E, PA:160E, PA-X:160E, PA:211I, PA-X:211Y, others, considered importance under One Health perspective. Similarly, N-linked sites (NLGs) both HA NA proteins found, highlighting loss/acquisition or some NLGs, such 209NNTN, 100 NPTT, 302NSSM (HA) 70NNTN, 68NISS, 50NGSV (NA). (4) Conclusions: provides our understanding about evolution current HPAIV circulating globally. These findings outline surveillance updating profiles these evolved viruses.

Language: Английский