Mycobacterium servetii sp. nov., a novel rapid-growing nontuberculous mycobacterial species recovered from a human patient in Zaragoza, Spain

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Journal Year: 2025, Volume and Issue: 75(3)

Published: March 19, 2025

In this study, we describe a novel rapid-growing Mycobacterium species isolated from clinical specimen obtained the lower respiratory tract of patient with ciliary dysfunction, bronchiectasis and exacerbated symptoms. A comprehensive phenotypic characterization was conducted, including establishment MALDI-TOF MS profile. Additionally, whole-genome sequencing performed to assess overall genomic relatedness indices conduct phylogenetic comparative analyses. These findings allowed us characterize previously unrecognized species, for which propose name servetii (=DSM 118141; =CECT 31091).

Language: Английский

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Clinical correlation study of non-tuberculous mycobacterial isolates from bronchoalveolar lavage fluid

AMB Express, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 5, 2025

Non-tuberculous mycobacterial (NTM) infections have emerged as a significant public health concern, posing threat to human health. This study aims identify various NTM strains from bronchoalveolar lavage fluid, assess their drug resistance profiles, and investigate the risk factors associated with disease. Gene chip technology was used strains. The broth microdilution method assessed sensitivity of isolated pathogenic bacteria, determining minimum inhibitory concentrations (MICs). Logistic regression analysis identified potential for Results showed slow-growing fluid be predominantly Mycobacterium avium intracellulare, accounting 32.05% 29.49% isolates, respectively. rapidly growing were mainly chelonae abscessus, each constituting 25.64% isolates. found sensitive clarithromycin, while linezolid demonstrated high antibacterial efficacy against intracellulare. In susceptibility testing amikacin exhibited highest sensitivity, followed by clarithromycin. For patients NTM-positive cultures, lung disease included age (45-60 years, > 60 years), smoking history exceeding 10 chronic obstructive pulmonary (COPD), bronchiectasis, immunocompromised status, presence thin-walled cavities. Collectively, this elucidates distribution strains, key disease, highlighting need proactive screening, early intervention, targeted preventive strategies improve diagnosis optimize treatment outcomes.

Language: Английский

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Evaluation of the Mycobacterium RealTime Kit Vircell (MRTVircell) Assay for Detecting Mycobacterium Species in Clinical Specimens

Pathogens, Journal Year: 2025, Volume and Issue: 14(5), P. 429 - 429

Published: April 28, 2025

Rapid and accurate diagnosis of mycobacterial infections is crucial for guiding therapeutic decisions. This study presents the first evaluation a novel molecular assay, Mycobacterium RealTime PCR Kit Vircell (MRTVircell), real-time PCR-based test designed specific detection tuberculosis complex (MTBC), avium (MAC), abscessus (MABC), other nontuberculous mycobacteria (NTM) in both respiratory non-respiratory samples. The was conducted under routine workflow conditions using 721 clinical specimens, including 559 162 Among these, 5.69% were smear-positive, 6.38% culture-positive MTBC, 9.85% NTM. performance MRTVircell compared to culture results Anyplex MTB/NTM assay. two systems demonstrated 96.95% overall concordance rate NTM, negative specimens. Based on as reference method, sensitivity, specificity, positive predictive value, value MTBC 80.43%, 99.64%, 94.87%, 98.41%, respectively, while (Seegene), these values 76.09%, 94.59%, 98.06%, respectively. For NTM detection, 28.17%, 99.29%, 83.33% 91.63% 21.13%, 99.11%, 75%, 91.67% MTB/NTM, rapid reliable tool differentiation MAC, MABC,

Language: Английский

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Biochip-Based Identification of Mycobacterial Species in Russia

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 13200 - 13200

Published: Dec. 8, 2024

Infections caused by nontuberculous mycobacteria (NTM) are rising globally throughout the world. The number of species isolated from clinical samples is steadily growing, which demands implementation a robust diagnostic method with wide specificity. This study was carried out in 2022-2024 three antituberculosis centers biggest cities Russia: Moscow, Saint Petersburg, and Novosibirsk. We developed DNA hybridization assay 'Myco-biochip' that allows identification 79 mycobacterial analyzed 3119 2221 patients. Sixty-eight were identified clinics, including novel phylogenetically related to

Language: Английский

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Rapid detection and differentiation of less common non-tuberculous mycobacteria using an in-house line probe assay

Gene, Journal Year: 2024, Volume and Issue: unknown, P. 149163 - 149163

Published: Dec. 1, 2024

Language: Английский

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