Respiratory and Enteric Bacterial Pathogens in Municipal Wastewater: A Potential Risk of Infection to Workers

Water, Journal Year: 2025, Volume and Issue: 17(2), P. 268 - 268

Published: Jan. 18, 2025

Investigating human pathogens in wastewater is crucial for identifying and predicting potential occupational health risks faced by treatment plant (WWTP) workers. This study aimed to determine the occurrence levels of Legionella pneumophila, Mycobacterium spp., Arcobacter butzleri, Aeromonas hydrophila untreated municipal wastewater. Grab influent, activated sludge, secondary settling tank (SST) effluent samples were collected bi-weekly over 6 months from 5 WWTPs Tshwane, South Africa. A. detected using quantitative PCR (qPCR), while was both a culture method qPCR. The four identified most at varying levels. pneumophila had positivity rate 92%, ranging 2 5.4 log10 MPN/100 mL. Detection rates L. serogroup 1 97%, 75%, 69%, respectively, with up 5.3 gene copies (GC)/mL. Importantly, this demonstrates molecular typing wastewater, topic that has been rarely documented. spp. all (log10 GC/mL) influent (2.8–7.6), sludge (4.8–8.9), SST (3.8–8.9) samples. butzleri 96% 82% samples, GC 0.8 6.6, 1.5 6.5, 0.7 6.6 GC/mL similar hydrophila. These findings underscore presence respiratory enteric various points, suggesting exposure WWTP emphasises need microbiological risk assessments (RAs) or reviewing existing RAs implementing necessary control measures protect

Language: Английский

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A Perspective on Wastewater and Environmental Surveillance as a Public Health Tool for Low- and Middle-Income Countries

Microorganisms, Journal Year: 2025, Volume and Issue: 13(2), P. 238 - 238

Published: Jan. 22, 2025

Geographical variations in infectious diseases create differences public health priorities between high- and low-income countries. Low- middle-income countries (LMICs) face resource constraints that limit adherence to international monitoring standards for wastewater-based epidemiology (WBE). The development of low-cost WBE programs, such as those detect SARS-CoV-2, offers LMICs a promising tool pathogens local concern. In this work, we summarize important wastewater biomarkers their associated challenges, ranging from causing gastroenteritis putative markers plant linked food safety, well antimicrobial resistance. We raise awareness the great potential highlight critical markers, research needs, strategies necessary establish tailored surveillance programs.

Language: Английский

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Unsupervised detection of novel SARS-CoV-2 mutations and lineages in wastewater samples using long-read sequencing

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: Jan. 29, 2025

Abstract The COVID-19 pandemic has underscored the importance of virus surveillance in public health and wastewater-based epidemiology (WBE) emerged as a non-invasive, cost-effective method for monitoring SARS-CoV-2 its variants at community level. Unfortunately, current variant methods depend heavily on updated genomic databases with data derived from clinical samples, which can become less sensitive representative testing sequencing efforts decline. In this paper, we introduce HERCULES ( H igh-throughput E pidemiological R econstruction C lustering U ncovering L ineages nvironmental S ARS-CoV-2), an unsupervised that uses long-read single 1 Kb fragment Spike gene. identifies quantifies mutations lineages without requiring database-guided deconvolution, enhancing detection novel variants. We evaluated Norwegian wastewater samples collected July 2022 to October 2023 part national pilot WBE SARS-CoV-2. Strong correlations were observed between sample terms prevalence lineages. Furthermore, found trends identified one week earlier than data. Our results demonstrate HERCULES’ capability identify new before their providing early warnings potential outbreaks. methodology described paper is easily adaptable other pathogens, offering versatile tool environmental emerging pathogens.

Language: Английский

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The Crucial Role of Laboratory Medicine in Addressing Future Public Health Infectious Threats: Insights Gained from the COVID-19 Pandemic

Diagnostics, Journal Year: 2025, Volume and Issue: 15(3), P. 323 - 323

Published: Jan. 30, 2025

Laboratory testing has played a pivotal role throughout the coronavirus disease 2019 (COVID-19) pandemic, exemplifying importance of in vitro diagnostics addressing public health threats posed by outbreaks infectious diseases. This article aims to present key insights from our expertise, derived evidence gathered during COVID-19 inform strategies for managing future challenges. Current scientific underscores that patient sample not only allows diagnose an acute severe respiratory syndrome 2 (SARS-CoV-2) infection, but also supports outbreak prediction, improved control measures, anticipation pressure on healthcare system, mitigation adverse clinical outcomes, and early detection emerging SARS-CoV-2 variants. Additionally, wastewater monitoring emerged as powerful tool forecasting burden, including both prevalence severity. Collectively, these findings underscore value diagnostic surveillance guiding planning optimizing resource allocation offering valid framework be applied threats, especially any potential “Disease X” may emerge future.

Language: Английский

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Informationsschatz aus Umweltproben

BIOspektrum, Journal Year: 2025, Volume and Issue: 31(1), P. 59 - 62

Published: Feb. 1, 2025

Language: Английский

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A new approach for the detection of genetic alterations utilizing modified loop-mediated isothermal amplification reaction (LAMP)

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 8, 2025

The increasing use of genetic testing for personalised therapy, highlights the need rapid, reliable diagnostics. Current methods are hindered by complex workflows, requiring advanced equipment, skilled personnel, and invasive tissue sampling. Loop-mediated isothermal amplification (LAMP) has emerged as a more efficient alternative to traditional PCR. LAMP eliminates thermal cycling, allowing faster, cost-effective tests, is less sensitive inhibitors, enabling from minimally processed samples. Although newer longer assay development time than PCR, its potential in oncology, particularly detecting changes, promising. We have developed LAMP-based method variations, optimized point-of-care testing. This technique uses modified primers with alterations at 3' end either F2 or B2 primers, ensuring specificity altered sequences. only produces positive signal when variant present, distinguishing it wild-type DNA. Our findings demonstrate that this high sensitivity, even samples both mutated material. Paired portable device, diagnostic could revolutionize alteration detection, offering quicker results improving treatment outcomes, targeted therapies.

Language: Английский

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Sensors for detection and monitoring of contaminants in wastewater

Current Opinion in Environmental Science & Health, Journal Year: 2025, Volume and Issue: unknown, P. 100609 - 100609

Published: March 1, 2025

Language: Английский

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A Quadruplex Digital PCR Assay for the Simultaneous Detection of Four Intestinal Bacterial Pathogens and Its Application in Wastewater Samples

Deleted Journal, Journal Year: 2025, Volume and Issue: 7(12), P. 393 - 399

Published: Jan. 1, 2025

Language: Английский

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Nano-engineered magnesium-enriched sugarcane bagasse for dual decontamination of heavy metals and E. coli in sewage water

Nano-Structures & Nano-Objects, Journal Year: 2025, Volume and Issue: 42, P. 101475 - 101475

Published: March 30, 2025

Language: Английский

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Environmental surveillance for Salmonella Typhi to detect the typhoid burden in Yogyakarta, Indonesia

International Journal of Hygiene and Environmental Health, Journal Year: 2025, Volume and Issue: 266, P. 114572 - 114572

Published: April 1, 2025

Language: Английский

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